Adult neurogenesis and specific replacement of interneuron subtypes in the mouse main olfactory bulb

Bagley, Joshua A.; LaRocca, Greg; Jiménez, Daniel; Urban, Nathaniel N.

doi:10.1186/1471-2202-8-92

Cited by 60 publications

(57 citation statements)

References 34 publications

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“…4, anti-DCX antibodies were visualized with Alexa Fluor 594, anti-GFP antibodies with Alexa Fluor 488 and antibodies against calbindin, calretinin, TH and NeuN were visualized with Alexa Fluor 680. This combination of antibodies was necessary, because many neurons in the glomerular layer do not express the usual marker of mature neurons NeuN 62 . After labelling, preparations were sequentially imaged at 800 nm excitation light using a 570LP dichroic mirror (thus separating fluorescence from Alexa Fluor 488 (blue in Supplementary Fig.…”

Section: Methodsmentioning

confidence: 99%

In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb

et al. 2015

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Section: Methodsmentioning

confidence: 99%

In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb

et al. 2015

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“…Batista-Brito et al (2008) suggested that neuronal composition in the GL dramatically changes during early postnatal life. Later on at young adulthood, Bagley et al (2007) showed that the various GL subpopulations are presented at similar proportions in the resident and the adult-born neurons, implying a stable neuronal composition in the GL. Whitman and Greer (2007) had similar results, with the exception of small over-representation of the DA neurons at this age.…”

Section: Turnover Of Dopaminergic Jgnsmentioning

confidence: 99%

Long-Term Imaging Reveals Dynamic Changes in the Neuronal Composition of the Glomerular Layer

Adam

Mizrahi²

2011

J. Neurosci.

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The mammalian olfactory bulb (OB) contains a rich and highly heterogeneous network of local interneurons (INs). These INs undergo continuous turnover in the adult OB in a process known as "adult neurogenesis." Although the overall magnitude of adult neurogenesis has been estimated, the detailed dynamics of the different subpopulations remains largely unknown. Here we present a novel preparation that enables long-term in vivo time-lapse imaging in the mouse OB through a chronic cranial window in a virtually unlimited number of sessions. Using this preparation, we followed the turnover of a specific neuronal population in the OB, the dopaminergic (DA) neurons, for as long as 9 months. By following the same population over long periods of time, we found clear addition and loss of DA neurons in the glomerular layer. Both cell addition and loss increased over time. The numbers of new DA cells were consistently and significantly higher than lost DA cells, suggesting a net increase in the size of this particular population with age. Over a 9 month period of adult life, the net addition of DA neurons reached ϳ13%. Our data argue that the fine composition of the bulbar IN network changes throughout adulthood rather than simply being replenished.

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“…1C). The neuron-specific nuclear protein NeuN is expressed by all mature granule cells in the GcL and by a subset of mature cells in the GL (Bagley et al, 2007), whereas DCX is expressed by all SVZ-derived neuroblasts and progressively downregulated as interneurons mature (Brown et al, 2003). The large majority of COUP-TFI + cells was co-labelled for NeuN in the GcL (85.7±3.5%; n=323 cells) and nearly 52% of COUP-TFI + cells did express NeuN in the GL (52.4±3.2%; n=330 cells).…”

Section: Coup-tfi Is Expressed In Mature Daergic Olfactory Interneuronsmentioning

confidence: 99%

COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons

et al. 2013

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COUP-TFI is an orphan nuclear receptor acting as a strong transcriptional regulator in different aspects of forebrain embryonic development. In this study, we investigated COUP-TFI expression and function in the mouse olfactory bulb (OB), a highly plastic telencephalic region in which continuous integration of newly generated inhibitory interneurons occurs throughout life. OB interneurons belong to different populations that originate from distinct progenitor lineages. Here, we show that COUP-TFI is highly expressed in tyrosine hydroxylase (TH)-positive dopaminergic interneurons in the adult OB glomerular layer (GL). We found that odour deprivation, which is known to downregulate TH expression in the OB, also downregulates COUP-TFI in dopaminergic cells, indicating a possible correlation between TH-and COUP-TFI-activitydependent action. Moreover, we demonstrate that conditional inactivation of COUP-TFI in the EMX1 lineage results in a significant reduction of both TH and ZIF268 expression in the GL. Finally, lentiviral vector-mediated COUP-TFI deletion in adult-generated interneurons confirmed that COUP-TFI acts cell-autonomously in the control of TH and ZIF268 expression. These data indicate that COUP-TFI regulates TH expression in OB cells through an activitydependent mechanism involving ZIF268 induction and strongly argue for a maintenance rather than establishment function of COUP-TFI in dopaminergic commitment. Our study reveals a previously unknown role for COUP-TFI in the adult brain as a key regulator in the control of sensory-dependent plasticity in olfactory dopaminergic neurons.

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Adult neurogenesis and specific replacement of interneuron subtypes in the mouse main olfactory bulb

Cited by 60 publications

References 34 publications

In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb

In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb

Long-Term Imaging Reveals Dynamic Changes in the Neuronal Composition of the Glomerular Layer

COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons

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