Adult Identity Crisis inLeucothrips(Thysanoptera: Thripidae) Associated with the Tropical Ornamental PlantCodiaeum variegatum(Euphorbiaceae)

Abstract

BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

A Preliminary Review of the Flower Thrips Frankliniella Karny (Thysanoptera: Thripidae) with Taxonomic Updates and Description of Five New Species

“…Genetic analysis.—Whole genomic DNA was extracted from available intercepted, and origin-collected specimens, using the non-destructive EDNA HiSp-ExTM tissue kit (Fisher Biotec, Wembly, Australia) (Skarlinsky et al 2017) or an equivalent generic “HotSHOT” method (Truett et al 2000). Subsequent to extraction, the polymerase chain reaction (PCR) was used to amplify a fragment of the mitochondrial cytochrome c oxidase subunit 1 (COI) from each specimen, using a protocol prescribed by Skarlinsky et al (2017). In addition, two regions of the nuclear ribosomal cistron, the complete internal transcribed spacer 2 (ITS2), and the D2 region of the 28S large subunit (28SD2) were intermittently amplified, again following Skarlinsky et al (2017).…”

“…Subsequent to extraction, the polymerase chain reaction (PCR) was used to amplify a fragment of the mitochondrial cytochrome c oxidase subunit 1 (COI) from each specimen, using a protocol prescribed by Skarlinsky et al (2017). In addition, two regions of the nuclear ribosomal cistron, the complete internal transcribed spacer 2 (ITS2), and the D2 region of the 28S large subunit (28SD2) were intermittently amplified, again following Skarlinsky et al (2017). Both loci are highly conserved within species, but typically differ between species; the former being much more divergent (in both length and nucleotide composition) than the latter (Hillis and Dixon 1991).…”

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

“…Haplotypes of H. nigricornis (Bagnall), H. sylvanus Faure, and S. aurantii Faure have also been identified based on variation in COI sequences [ 50 , 110 ]. Leucothrips furcatus Hood has been separated into two clades that show differences in reproductive and feeding behavior [ 111 ]. The existence of such a large number of morphologically indistinguishable invasive species raises practical concerns in thrips diagnosis, monitoring, and management.…”