Adult human neural crest–derived cells for articular cartilage repair

Pelttari, Karoliina; Pippenger, Benjamin E.; Mumme, Marcus; Feliciano, Sandra; Scotti, Celeste; Mainil‐Varlet, Pierre; Procino, Alfredo; Rechenberg, Brigitte von; Schwamborn, Thomas; Jakob, Marcel; Cillo, Clemente; Barbero, Andrea; Martin, Ivan

doi:10.1126/scitranslmed.3009688

Cited by 98 publications

(82 citation statements)

References 63 publications

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“…Recently, a new promising cell niche for articular cartilage regeneration was investigated by Pelttari et al 115 The authors showed that adult human neuroectoderm-derived nasal chondrocytes (NCs) can be constitutively distinguished from mesoderm-derived articular chondrocytes (ACs) by lack of expression of specific HOX genes, including HOXC4 and HOXD8. In contrast to ACs, serially cloned NCs could be continuously reverted from differentiated to dedifferentiated states, conserving the ability to form cartilage tissue in vitro and in vivo.…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

Cartilage and Bone Regeneration—How Close Are We to Bedside?

Canadas¹,

Pina²,

Marques³

et al. 2016

Translating Regenerative Medicine to the Clinic

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Key ConceptsThis chapter begins by presenting bone and cartilage physiology and disorders concepts and currently applied treatments, including their limitations and the potential solutions proposed within tissue engineering and regenerative medicine. Next, biomimetic strategies for bone and cartilage tissue engineering, namely scaffolds, hydrogels, and fibers, are presented, as well as natural and synthetic biomaterials, such as polymers, bioactive inorganic materials and composites, employed for such processing methodologies. Lastly, a thorough review of ongoing clinical trials and commercial products that are already in the market for osteochondral tissue regeneration are also presented.

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Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

Cartilage and Bone Regeneration—How Close Are We to Bedside?

Canadas¹,

Pina²,

Marques³

et al. 2016

Translating Regenerative Medicine to the Clinic

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show abstract

“…Other cell sources that have been considered are fat stromal cells and umbilical cord cells although no clinical trial has been published so far. An interesting approach using nasal septal cartilage that is derived from the neural ridge has been demonstrated to have good cartilage regenerating capacity in vitro and there are ongoing clinical trials with promising results [43].…”

Section: The Treatment Of Osteoarthritis and Cartilage Defectsmentioning

confidence: 99%

From gristle to chondrocyte transplantation: treatment of cartilage injuries

Lindahl¹

2015

Phil. Trans. R. Soc. B

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This review addresses the progress in cartilage repair technology over the decades with an emphasis on cartilage regeneration with cell therapy. The most abundant cartilage is the hyaline cartilage that covers the surface of our joints and, due to avascularity, this tissue is unable to repair itself. The cartilage degeneration seen in osteoarthritis causes patient suffering and is a huge burden to society. The surgical approach to cartilage repair was nonexisting until the 1950s when new surgical techniques emerged. The use of cultured cells for cell therapy started as experimental studies in the 1970s that developed over the years to a clinical application in 1994 with the introduction of the autologous chondrocyte transplantation technique (ACT). The technology is now spread worldwide and has been further refined by combining arthroscopic techniques with cells cultured on matrix (MACI technology). The non-regenerating hypothesis of cartilage has been revisited and we are now able to demonstrate cell divisions and presence of stem-cell niches in the joint. Furthermore, cartilage derived from human embryonic stem cells and induced pluripotent stem cells could be the base for new broader cell treatments for cartilage injuries and the future technology base for prevention and cure of osteoarthritis.

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“…Nasal septal cartilage, previously labelled as the pacemaker for orofacial cartilage tissue regeneration (Pelttari et al, 2014) could potentially provide for an attractive non-disc source of cells as it contains a large population of differentiated chondrocytes (Kafienah et al, 2002). Nasal chondrocytes (NCs) (Bujia, 1995;Kafienah et al, 2002;Richmon et al, 2005) have been used to engineer cartilaginous constructs both in vitro and in vivo (Asawa et al, 2009;Candrian et al, 2008;Chia et al, 2005;Fulco et al, 2014;Hellingman et al, 2011;Malda et al, 2004;Richmon et al, 2005;Rotter et al, 2001Rotter et al, , 2002Scotti et al, 2012;Tsaryk et al, 2017;Twu et al, 2014;Watson and Reuther, 2014) and have recently been explored for nucleus pulposus tissue regeneration (Tsaryk et al, 2015;Tsaryk et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro extracellular matrix accumulation of nasal and articular chondrocytes for intervertebral disc repair

Vedicherla

Buckley

2017

Tissue and Cell

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Chondrocyte based regenerative therapies for intervertebral disc repair such as Autologous Disc Cell Transplantation (ADCT, CODON) and allogeneic juvenile chondrocyte implantation (NuQu®, ISTO Technologies) have demonstrated good outcomes in clinical trials. However concerns remain with the supply demand reconciliation and issues surrounding immunoreactivity which exist for allogeneic-type technologies. The use of stem cells is challenging due to high growth factor requirements, regulatory barriers and differentiation towards a stable phenotype. Therefore, there is a need to identify alternative non-disc cell sources for the development and clinical translation of next generation therapies for IVD regeneration. In this study, we compared Nasal Chondrocytes (NC) as a non-disc alternative chondrocyte source with Articular Chondrocytes (AC) in terms of cell yield, morphology, proliferation kinetics and ability to produce key extracellular matrix components under 5% and 20% oxygen conditions, with and without exogenous TGF-β supplementation.Results indicated that NC maintained proliferative capacity with high amounts of sGAG and lower collagen accumulation in the absence of TGF-β supplementation under 5% oxygen conditions. Importantly, osteogenesis and calcification was inhibited for NC when cultured in IVD-like microenvironmental conditions. The present study provides a rationale for the exploration of nasal chondrocytes as a promising, potent and clinically feasible autologous cell source for putative IVD repair strategies.

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Adult human neural crest–derived cells for articular cartilage repair

Cited by 98 publications

References 63 publications

Cartilage and Bone Regeneration—How Close Are We to Bedside?

Cartilage and Bone Regeneration—How Close Are We to Bedside?

From gristle to chondrocyte transplantation: treatment of cartilage injuries

In vitro extracellular matrix accumulation of nasal and articular chondrocytes for intervertebral disc repair

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