Adult hippocampal neurogenesis in neuropathic pain and alkyl glycerol ethers treatment

Tyrtyshnaia, Anna; Manzhulo, Igor; Sultanov, Ruslan; Ermolenko, Ekaterina V.

doi:10.1016/j.acthis.2017.10.007

Cited by 24 publications

(37 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We do not exclude the possibility that an increase in the length and complexity of dendrites in the DG granule neurons occurs as a compensatory reaction to a decrease in the number of newly formed DCX + neurons and a decrease in hippocampal neurogenesis, which we observed earlier in neuropathic pain animals. 29,35,43 An increase in the dendritic tree complexity with a decrease in hippocampal neurogenesis explains the absence of short-term memory impairment. In addition, it is known that pathologies accompanied by chronic stress, 20,40 including chronic neuropathic pain, are characterized by increased GABA release within the hippocampus.…”

Section: Discussionmentioning

confidence: 99%

“…Given the key role of the hippocampal formation in cognitive functions, learning and memory, studying morphological, functional and plastic changes can help in the further search for the effective therapeutic agents to eliminate neuropathic pain consequences. The studies using various neuropathic pain models demonstrate changes in the hippocampus including impaired LTP, 24 biochemical changes 31 and impaired hippocampus-dependent functions, including working, 43 shortterm 34 and recognition memory. 12 In this study, we focused on changes in neuronal plasticity, which are the anatomical substrate of the observed functional changes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

<p>Neuropathic Pain Causes Memory Deficits and Dendrite Tree Morphology Changes in Mouse Hippocampus</p>

Tyrtyshnaia¹,

Manzhulo²

2020

JPR

View full text Add to dashboard Cite

Introduction: Neuropathic pain manifests in a diverse combination of sensory symptoms and disorders of higher nervous activity, such as memory deficiency, anxiety, depression, anhedonia, etc. This suggests the participation of brain structures, including the hippocampus, in the pathogenesis of neuropathic pain. The elucidation of central sensitization mechanisms underlying neuropathic pain cognitive and affective symptoms may be useful in the development of new and effective treatments for these common disorders. The study aims to elucidate the effect of chronic neuropathic pain on cognitive function and underlying neuronal plasticity in the hippocampus. Methods: Chronic constriction injury of mouse right hind limb sciatic nerve was used as a model of neuropathic pain. The presence of neuropathic pain was confirmed by the thermal and mechanical allodynia. The morphology of the CA1 pyramidal neurons and the dentate gyrus (DG) granule neurons were studied using Golgi-Cox staining. The hippocampal proteins concentration was determined by immunohistochemistry and ELISA. Results: Behavioral testing revealed reduced locomotor activity as well as impaired working and long-term memory in mice with a ligated nerve. We revealed changes in the dendritic tree morphology in CA1 and the dentate gyrus hippocampal subregions. We found the atrophy of the CA1 pyramidal neurons and an increase in the dendritic tree complexity in DG. Moreover, changes in the density of dendritic spines were observed in these regions. In addition, we revealed increased expression of the Arc protein in DG granule neurons and decreased surface expression of AMPA receptors within the hippocampus. Decreased AMPA receptors expression underlies observed altered dendrite arborization and dendritic spines morphology. Discussion: We found that pain information entering the hippocampus causes neuronal plasticity changes. The changes in neurite arborization, dendritic length and dendritic spines morphology as well as protein expression are observed within the hippocampal regions involved in the processing of pain information. Moreover, changes in the dendrite morphology in hippocampal subregions are different due to the anatomical and functional heterogeneity of the hippocampus. Apparently, the detected morphological and biochemical changes can underlie the observed hippocampus-dependent behavioral and cognitive impairment in animals with neuropathic pain.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Neuropathic Pain Causes Memory Deficits and Dendrite Tree Morphology Changes in Mouse Hippocampus</p>

Tyrtyshnaia¹,

Manzhulo²

2020

JPR

View full text Add to dashboard Cite

show abstract

“…Experiments were performed as previously described (19) using 28 18-month old male C57BL/6 mice with an average weight of 45 g. The animals were housed 2-4 per cage with a 12-h light/dark cycle and had ad libitum access to food and water. In order to reduce the amount of stress place on the animals, the mice were handled for only 5 min once a day over a period of 5 consecutive days prior to the experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The liver from the squid Berryteuthis magister was obtained from Nakhodka Active Marine Fishery Base (Nakhodka, Russia) and stored at −200°C. The extraction of total lipids was performed as previously described (19) and as described in the study by Bligh and Dyer (1959); the saponification of lipids was carried out using a conventional technique (21). Following hydrolysis and acidification, the lipid mixture was dissolved in acetone at a ratio of 1:5 at room temperature and incubated for 24 h at −20°C, as previously described (22).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Neuroinflammation and adult hippocampal neurogenesis in neuropathic pain and alkyl�glycerol ethers treatment in aged mice

et al. 2019

View full text Add to dashboard Cite

Neuropathic pain is a condition characterized by unpleasant sensory and emotional experiences associated with a number of diseases or injuries affecting the sensory system through various mechanisms. In this study, we focused on the impact of chronic neuropathic pain on the microglial state and hippocampal neurogenesis in aged mice. In addition, we examined the effects of alkyl glycerol ethers (AGE) treatment on behavioral parameters, hippocampal neuronal and microglial plasticity in aged C57BL/6 mice with neuropathic pain. For the induction of neuropathic pain, we used the model of chronic constriction injury (CCI) of the sciatic nerve. We observed painful behavior in animals subjected to CCI, expressed as a decrease in locomotor activity and the development of cold allodynia. A violation of working and long-term memory was also observed. AGE administration reduced the severity of cold allodynia and prevented memory impairment. In addition to behavioral changes, neuropathic pain was accompanied by microglial activation, changes in the hippocampal production of pro- and anti-inflammatory cytokines, as well as a decrease in neurogenesis. The administration of AGE prevented the neuropathic pain-derived effects, including M1 microglial activation and neurogenesis disruption. However, in vitro experiments demonstrated the pro-inflammatory activation of microglial cells, emphasizing the complexity of the mechanisms underlying the pharmacological effects of AGE. On the whole, the findings of this study demonstrate that AGE treatment prevented behavioral effects of neuropathic pain in mice, and AGE may thus have potential for use in the prevention or treatment of neuropathic pain cognitive and emotional effects. However, as the mechanisms underlying this type of pain are complex, further studies are required to determine the detailed pharmacological effects of AGE.

show abstract

New Cytotoxic Monoalkyl Glycerol Ether from the Red Sea Soft Coral Nephthea mollis

Eissa,

Abdel‐Tawab,

Hamed

et al. 2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

A new monoalkyl glycerol ether, 3‐(n‐henicosyloxy) propane‐1,2‐diol (1), was isolated from the CH2Cl2/MeOH crude extract of the Red Sea soft coral Nephthea mollis. Additionally, three known related analogues were identified: chimyl alcohol (2), batyl alcohol (3), and 3‐(icosyloxy) propane‐1,2‐diol (4). The chemical structure of 3‐(n‐henicosyloxy) propane‐1,2‐diol was determined using advanced spectroscopic analyses, including 1D, 2D Nuclear Magnetic Resonance (NMR), Electron Ionization mass spectra (EI‐MS), and High‐Resolution Electron Spray Ionization mass spectra (HRESIMS) analyses. Furthermore, the identification of chimyl alcohol, batyl alcohol and 3‐(icosyloxy) propane‐1,2‐diol was achieved by studying their EI mass fragmentation analyses and comparing their mass data with those previously reported in the literature. The cytotoxic activity of the Nephthea mollis crude extract and 3‐(n‐henicosyloxy) propane‐1,2‐diol was evaluated against five human cancer cell lines: HepG2 (hepatocellular carcinoma), MCF‐7 (breast carcinoma), NCI‐1299 (lung carcinoma), HeLa (cervical cancer cell), and HT‐29 (colon adenocarcinoma). Moreover, 3‐(n‐henicosyloxy) propane‐1,2‐diol revealed moderate cytotoxicity against the HeLa cell line with an IC50 value of 24.1 μM, while showing inactivity against the remaining cell lines (IC50 >100 μM).

show abstract

Adult hippocampal neurogenesis in neuropathic pain and alkyl glycerol ethers treatment

Cited by 24 publications

References 61 publications

<p>Neuropathic Pain Causes Memory Deficits and Dendrite Tree Morphology Changes in Mouse Hippocampus</p>

<p>Neuropathic Pain Causes Memory Deficits and Dendrite Tree Morphology Changes in Mouse Hippocampus</p>

Neuroinflammation and adult hippocampal neurogenesis in neuropathic pain and alkyl�glycerol ethers treatment in aged mice

New Cytotoxic Monoalkyl Glycerol Ether from the Red Sea Soft Coral Nephthea mollis

Contact Info

Product

Resources

About