Adult functions for the <i>Drosophila</i> DHR78 nuclear receptor

Marxreiter, Stefanie; Thummel, Carl S.

doi:10.1002/dvdy.24608

Cited by 2 publications

(4 citation statements)

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“…We designated it BdHR78 (because it was most closely related to D. melanogaster HR78) and C. capitata HR78, both of which belong to the NR2D group. HR78 was found to be involved in regulating the expression of genes in the ecdysone and Notch signaling pathways [16]. The functions of BdHR78 have not been reported; however, our qRT-PCR analysis indicated that BdHNF4 and BdHR78 were expressed in the midgut of third instar larvae, which indicated that these two genes may have some similar functions ( Figure 5).…”

Section: Nr2 Subfamilymentioning

confidence: 71%

“…They play a key role in the physiological processes related mainly to embryo formation, neurodevelopment, metabolism, and detoxification. For example, the nuclear hormone receptor HR96 mediates resistance to foreign compounds [15] and HR78 plays a major role in promoting the expression of genes in the midgut, suggesting it may contribute to nutrient uptake [16].The oriental fruit fly (Bactrocera dorsalis) is a devastating and adaptable insect (Diptera: Tephritidae) with a wide range of food sources that include 450 different kinds of fruits and vegetables. This pest poses a serious threat to the fruit and vegetable industry and can cause very serious economic losses [17].…”

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Molecular Characterization and Expression Profiling of Nuclear Receptor Gene Families in Oriental Fruit Fly, Bactrocera Dorsalis (Hendel)

Yang

Chen

Song

et al. 2020

Insects

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The oriental fruit fly (Bactrocera dorsalis) is a pest that causes large economic losses in the fruit and vegetable industry, so its control is a major challenge. Nuclear receptors (NRs) are a superfamily of ligand-dependent transcription factors that directly combine with DNA to regulate the expression of downstream target genes. NRs are closely associated with multiple physiological processes such as metabolism, reproduction, and development. Through sequence searches and analysis, we identified 21 B. dorsalis NR genes, all of which contained at least one of the two characteristic binding domains. On the basis of the conserved sequences and phylogenetic relationships, we divided the 21 NR genes into seven subfamilies. All members of the NR0 subfamily and BdHR83, which belonged to the NR2E group, lacked ligand-binding domains. The BdDSF and BdHR51, which also belonged to the NR2Egroup, and BdE78 (which belonged to the NR1E group) all lacked DNA-binding domains. The BdDSF and BdHR83 sequences were incomplete, and were not successfully amplified. Development-and tissue-specific expression profiling demonstrated that the transcript levels of the 19 NR genes varied considerably among eggs, larva, pupae, and adults, as well as among larval and adult male and female tissues. Our results will contribute to a better understanding of NR evolution and expand our knowledge of B. dorsalis physiology.Insects 2020, 11, 126 2 of 15 in recognizing hormone response elements. The D domain functions as a hinge between the DBD and the ligand-binding domain (LBD). The LBD (also known as E domain) contains a hydrophobic ligand-binding pocket that is specific to each receptor and is the source of the sequence variability within the LBD. In addition, the LBD domain mediates dimerization and the ligand-dependent transcriptional activation function (AF-2) [4]. The C-terminal contains the F domain, which is not present in all NRs and is highly diverse.A large number of NR gene sets have been characterized from the whole-genome sequence of many animals. For example, 48 NRs were identified in the human genome [5], over 270 NRs have been found in Caenorhabditis elegans [6], and 49 and 47 were found in mouse and rat, respectively [7]. Surprisingly, only 21 NRs were found in Drosophila melanogaster [8], 19 in Bombyx mori [9], 22 in Apis mellifera [10], and 21 in Tribolium castaneum [11].In D. melanogaster, seven NR genes that are regulated directly by 20E have been identified, namely EcR, E75, E78, FTZ-F1, HR3, HR4, and HR39 [12]. Among them, E75 is the primary response gene of 20E. The others are secondary response genes that are maximally expressed after 20E-induced protein synthesis has begun [13]. The ligand-receptor complex of 20E-EcR-USP induces the expression of the 20E primary response gene. Then, the cascade of 20E secondary response genes, which is induced by the transcription factor encoded by the 20E primary response gene, amplifies the 20E signal by regulating the expression of the secondary response genes (e.g., HR3, HR4, and FTZ...

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Section: Nr2 Subfamilymentioning

confidence: 71%

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confidence: 99%

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confidence: 99%

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Molecular Characterization and Expression Profiling of Nuclear Receptor Gene Families in Oriental Fruit Fly, Bactrocera Dorsalis (Hendel)

Yang

Chen

Song

et al. 2020

Insects

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“…Testicular receptors 4 (TR4) mutants also display reproductive defects consistent with their abundant expression in the testes and ovary. These include defects in spermatogenesis and reduced male fertility, as well as reduced female fertility, smaller ovaries, and defects in follicle development [33] Other major upregulated gene categories correspond to defense response pathways suggesting that DHR78 mutants have an active stress response [34] The methanolic extract of CN have clearly shown an indirect dose dependent improvement in fertility (Fig. 4).…”

Section: Discussionmentioning

confidence: 99%

Effect of Methanolic Extract of Caryota no Seed on Geotactic Behaviour and Fecundity in Drosophila melanogaster

Maduagwuna

Omale

Etuh

et al. 2020

JOCAMR

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Aims: To investigate the effect of the methanolic extracts of Caryota no (CN) seeds negative geotaxis, fecundity and acetylcholinesterase (AChE) using Drosophila melanogaster (DM). Study Design: Experimental design. Place and Duration: Sample: African Centre of Excellence for Phytomedicine Research and Development, University of Jos, Jos Plateau State Nigeria between June 2018 and February 2019 Methodology: 50 flies were exposed in each vial to the following concentrations: 300 mg, 350 mg, 400 mg, 500 mg and 600 mg of methanolic extracts in 5 replicates for 7 days with daily recording of mortality. Total protein assays were carried out by Randox method from the supernatant from homogenized whole flies. In vivo antioxidant activity study was conducted by measuring level of acetylcholinesterase (AChE) enzyme activity from supernatants of whole fly homogenates using a spectrophotometer at specific wavelengths over a 2 minute duration. The values were derived as part of the total protein value. Negative geotaxis was done by the climbing assay and fecundity was examined by rate of emergence of larva after exposure of the flies to treatment. The statistical difference among test groups was presumed at P < .05. Results: The methanolic extract of CN caused nonsignificant (P = .33) decrease in total protein levels compared to the control. There were also nonsignificant decreases in AChE (P = .30) activity, negative geotactic (P = .80) behaviour and nonsignificant increase in fecundity (P = .17) in the methanolic extract-treated flies compared to the controls. Conclusion: It can therefore be concluded that the methanolic extract of Caryota no nonsignificantly improved fertitity and reduced negative geotaxis and AChE activity in DM.

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Adult functions for the Drosophila DHR78 nuclear receptor

Abstract: This study provides a new context for linking the molecular activity of the TR orphan nuclear receptors with their complex roles in adult physiology and lifespan. Developmental Dynamics 247:315-322, 2018. © 2017 Wiley Periodicals, Inc.

Cited by 2 publications

References 34 publications

Molecular Characterization and Expression Profiling of Nuclear Receptor Gene Families in Oriental Fruit Fly, Bactrocera Dorsalis (Hendel)

Molecular Characterization and Expression Profiling of Nuclear Receptor Gene Families in Oriental Fruit Fly, Bactrocera Dorsalis (Hendel)

Effect of Methanolic Extract of Caryota no Seed on Geotactic Behaviour and Fecundity in Drosophila melanogaster

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