Adult bone-marrow-derived mesenchymal stem cells contribute to wound healing of skin appendages

Abstract

Adult bone-marrow-derived mesenchymal stem cells (MSCs) are well-established as having the capacity to differentiate into cells with mesodermal, ectodermal, and endodermal characteristics and can leave their niche to home toward and engraft within foreign tissues. To investigate whether adult MSCs contribute to the repair of skin appendages after injury, BrdU-labeled MSCs were cocultured with heat-shocked confluent sweat gland cells (SGCs) in vitro and later intravenously injected into fullthickness skin wounds in rats. When adult MSCs were cocultured with heat-shocked SGCs, a subset of adult MSCs differentiated into SGCs, the percentage of differentiation being enhanced by epidermal growth factor and the injured microenviroment, but weakened by PD98059. The ERK (extracellular signal-regulated kinase) pathway, especially pERK, was involved in the phenotype conversion of human MSCs into human SGC. Labeled MSCs were noted in hair follicles, sebaceous glands, blood vessels, and dermis in full-thickness wounds, and the incorporated cells in hair follicles and sebaceous glands were also positive for pancytokeratin. After wound healing, some labeled MSCs returned to the bone marrow, whereas other were retained in the dermis. We conclude that adult MSCs have the capacity to dock at specific sites, to contribute to wound healing of skin appendages, and to home toward marrow, and that engraftment of bone-marrow-derived cells is a functional event.

Transplanted Adipose Derived Mesenchymal Stem Cells Attenuate The Acute Renal Injury Induced by Cisplatin in Rats

“…Positive nuclear reactions appeared brown in color. Negative control was performed using the 2 nd antibody only to check for any cross reaction [23] .…”

Role of Mesenchymal Stem Cells Versus their Conditioned Medium on Cisplatin-Induced Acute Kidney Injury in Albino Rat. A Histological and Immunohistochemical Study

“…Immunohistochemical characterization was performed by using streptavidin-biotin immunoperoxidase technique for CD44, CD 105, CD 45 and CD 34 [12] .…”

“…-Subgroup IIb: rats were sacrificed after three days from cisplatin injection (4 th day of the experiment) [12] .…”

Ameliorative Role of Bone Marrow Mesenchymal Stem Cells and Rutin Against Acetaminophen Induced Hepatotoxicity in Adult Male Rats.

“…After centrifugation, the cells were re-suspended and counted with trypan blue stain 0.4%(100μL of cell suspension with 100μL of stain) using Neubauer haemocytometer as described by Belsey et al,1980 MSCs were distinguished from other BM cells by their tendency to adhere to tissue culture plastic (Mok et al,2003), and by negative expression of CD34 and positive expression of CD44 (Fig. 1) in immune-staining, described by Li et al,2006. Briefly, the cultured cells were fixed in petri dishes on day 12 of culture by Acetone: Methanol (1:1) then covered by H2O2 (10%).…”