Adult and Larval Tracheal Systems Exhibit Different Molecular Architectures in Drosophila

Bossen, Judith; Prange, R.; Kühle, Jan-Philip; Künzel, Sven; Niu, Xiao; Hammel, Jörg U.; Krieger, Laura; Knop, Mirjam; Ehrhardt, Birte; Uliczka, Karin; Krauss‐Etschmann, Susanne; Roeder, Thomas

doi:10.3390/ijms24065628

Cited by 4 publications

(2 citation statements)

References 69 publications

(79 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During pupal metamorphosis, airway progenitor cells, the tracheoblasts, give rise to the adult tracheal system by using parts of the larval system as scaffold 51 . Of note, the airway system of adult flies is much more complex than that of larvae 52 . Having shown that development is disturbed in gene-modified flies, we next monitored the lifespan of male and female adult flies until natural death occurred.…”

Section: Resultsmentioning

confidence: 99%

Airway specific deregulation of asthma-related serpins impairs tracheal architecture and oxygenation in D. melanogaster

Ehrhardt,

Angstmann,

Höschler

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Serine proteases are important regulators of airway epithelial homeostasis. Altered serum or cellular levels of two serpins, Scca1 and Spink5, have been described for airway diseases but their function beyond antiproteolytic activity is insufficiently understood. To close this gap, we generated fly lines with overexpression or knockdown for each gene in the airways. Overexpression of both fly homologues of Scca1 and Spink5 induced the growth of additional airway branches, with more variable results for the respective knockdowns. Dysregulation of Scca1 resulted in a general delay in fruit fly development, with increases in larval and pupal mortality following overexpression of this gene. In addition, the morphological changes in the airways were concomitant with lower tolerance to hypoxia. In conclusion, the observed structural changes of the airways evidently had a strong impact on the airway function in our model as they manifested in a lower physical fitness of the animals. We assume that this is due to insufficient tissue oxygenation. Future work will be directed at the identification of key molecular regulators following the airway-specific dysregulation of Scca1 and Spink5 expression.

show abstract

Section: Resultsmentioning

confidence: 99%

Airway specific deregulation of asthma-related serpins impairs tracheal architecture and oxygenation in D. melanogaster

Ehrhardt,

Angstmann,

Höschler

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…A role for ELKS in peptide release has been described for insulin secretion in pancreatic β-cells (Ohara-Imaizumi et al, 2019). Yet, peptidergic ecdysis-triggering hormone (ETH) release from the endocrine epitracheal cells also appeared to be BRPindependent (unpublished data), though BRP is expressed by those cells (Bossen et al, 2023).…”

Section: Discussionmentioning

confidence: 97%

The neuropeptide pigment‐dispersing factor signals independently of Bruchpilot‐labelled active zones in daily remodelled terminals of Drosophila clock neurons

Hofbauer,

Zandawala,

Reinhard

et al. 2024

Eur J of Neuroscience

View full text Add to dashboard Cite

The small ventrolateral neurons (sLNvs) are key components of the central clock in the Drosophila brain. They signal via the neuropeptide pigment‐dispersing factor (PDF) to align the molecular clockwork of different central clock neurons and to modulate downstream circuits. The dorsal terminals of the sLNvs undergo daily morphological changes that affect presynaptic sites organised by the active zone protein Bruchpilot (BRP), a homolog of mammalian ELKS proteins. However, the role of these presynaptic sites for PDF release is ill‐defined.Here, we combined expansion microscopy with labelling of active zones by endogenously tagged BRP to examine the spatial correlation between PDF‐containing dense‐core vesicles and BRP‐labelled active zones. We found that the number of BRP‐labelled puncta in the sLNv terminals was similar while their density differed between Zeitgeber time (ZT) 2 and 14. The relative distance between BRP‐ and PDF‐labelled puncta was increased in the morning, around the reported time of PDF release. Spontaneous dense‐core vesicle release profiles of sLNvs in a publicly available ssTEM dataset (FAFB) consistently lacked spatial correlation to BRP‐organised active zones. RNAi‐mediated downregulation of brp and other active zone proteins expressed by the sLNvs did not affect PDF‐dependent locomotor rhythmicity. In contrast, down‐regulation of genes encoding proteins of the canonical vesicle release machinery, the dense‐core vesicle‐related protein CADPS, as well as PDF impaired locomotor rhythmicity.Taken together, our study suggests that PDF release from the sLNvs is independent of BRP‐organised active zones, while BRP may be redistributed to active zones in a time‐dependent manner.

show abstract

PDF neuropeptide signals independently of Bruchpilot-labelled active zones in daily remodelled terminals ofDrosophilaclock neurons

Hofbauer

Zandawala

Reinhard

et al. 2023

Preprint

View full text Add to dashboard Cite

The small ventrolateral neurons (sLNvs) are key components of the central clock in the Drosophila brain. They signal via the neuropeptide Pigment-dispersing factor (PDF) to align the molecular clockwork of different central clock neurons and to modulate downstream circuits. The dorsal terminals of the sLNvs undergo daily morphological changes that have been shown to affect presynaptic sites organised by the active zone protein Bruchpilot (BRP), a homolog of mammalian ELKS proteins. Although the circadian plasticity of the sLNv terminals is well established, whether and how it is related to the rhythmic release of PDF remains ill-defined. Here, we combined expansion microscopy with labelling of active zones by endogenously tagged BRP to examine the spatial correlation between PDF-containing dense-core vesicles and BRP-labelled active zones. We found that the number of BRP-labelled punctae in the sLNv terminals remained stable while their density changed during circadian plasticity. The relative distance between BRP- and PDF-labelled punctae was increased in the morning, around the reported time of PDF release. Spontaneous dense-core vesicle release profiles of the sLNvs in a publicly available ssTEM dataset (FAFB) consistently lacked spatial correlation to BRP-organised active zones. RNAi-mediated downregulation of brp and other active zone proteins expressed by the sLNvs did not affect PDF-dependent locomotor rhythmicity. In contrast, down-regulation of genes of the canonical vesicle release machinery, the dense-core vesicle-related protein CADPS, as well as PDF impaired locomotor rhythmicity. Taken together, our study suggests that PDF release from the sLNvs is independent of BRP-organised active zones which seem not to be circadianly destroyed and re-established.

show abstract

Adult and Larval Tracheal Systems Exhibit Different Molecular Architectures in Drosophila

Cited by 4 publications

References 69 publications

Airway specific deregulation of asthma-related serpins impairs tracheal architecture and oxygenation in D. melanogaster

Airway specific deregulation of asthma-related serpins impairs tracheal architecture and oxygenation in D. melanogaster

The neuropeptide pigment‐dispersing factor signals independently of Bruchpilot‐labelled active zones in daily remodelled terminals of Drosophila clock neurons

PDF neuropeptide signals independently of Bruchpilot-labelled active zones in daily remodelled terminals ofDrosophilaclock neurons

Contact Info

Product

Resources

About