“…Rats injected with CFA exhibited pronounced thermal hyperalgesia that developed within 1 day and persisted through 14 days following injection. This observation is similar to that of previous reports on the temporal profile of thermal hyperalgesia in CFA-induced chronic pain model (Wang et al, 2009 ; Li et al, 2012 ). Three-way ANOVA revealed that PWLs were significantly modulated by “group” ( F (1,19) = 5.8, p = 0.03), “time” ( F (5,95) = 7.5, p < 0.0001), “stimulation site” ( F (1,19) = 43.8, p < 0.0001), interactions between two factors (“group” × “stimulation site”: F (1,19) = 41.0, p < 0.0001 ; “time” × “stimulation site”: F (5,95) = 9.5, p < 0.0001; “group” × “time”: marginal significance, F (5,95) = 2.2, p = 0.06), and the interaction between three factors ( F (5,95) = 7.6, p < 0.0001).…”