Adsorptive stripping voltammetry of biomacromolecules with transfer of the adsorbed layer

Paleček, Emil; Postbieglová, Irena

doi:10.1016/0022-0728(86)80108-5

Cited by 175 publications

(84 citation statements)

References 7 publications

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“…Application of the adsorptive transfer (medium exchange) stripping techniques [21,36,37,48,49], not included in this article, makes it possible to reduce the volume of the analyzed solution to several mL decreasing thus substantially the amount of the analyte necessary for the analysis. Further work will be necessary using model systems to obtain information about the changes in electrochemical responses resulting, e.g., from formation of a specific DNA-protein complex, about the stability of such a complex at the electrode surface and about the optimum experimental conditions for the given system, etc.…”

Section: Electrochemical Analysis In Protein-protein and Dna-protein mentioning

confidence: 99%

Constant Current Chronopotentiometric Stripping Analysis of Bioactive Peptides at Mercury and Carbon Electrodes

et al. 1998

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Constant current derivative chronopotentiometric stripping analysis (CPSA) was used to study bioactive peptides [Lys 8 ]-vasopressin and angiotensin II at carbon paste electrode (CPE) and hanging mercury drop electrode (HMDE). Both peptides contain a single tyrosine residue which is oxidized at CPE close to þ0.6 V (against Ag/AgCl/3 M KCl electrode) producing peak Y; this peak was by about 15 mV more positive in vasopressin than in angiotensin II. At HMDE vasopressin yielded peak S due to the reduction of the disulfidic bond in the peptide. No peak S was observed with angiotensin II as it does not contain any cystine/cysteine residues. Vasopressin produced different catalytic hydrogen reduction signals in media with cobalt ion and in media not containing any metal ion. The signal produced in the latter media (peak H), which appeared close to ¹1.7 V, was well separated from the background. No catalytic hydrogen reduction signal was obtained with angiotensin II. The above mentioned CPSA signals can be applied for the determination of the respective peptide at nanomolar concentrations. The least sensitive peak S appears which requires at least 100 nM vasopressin concentration at moderate accumulation time. By two orders of magnitude, higher sensitivities can be obtained with peaks Y and H. In measuring these peaks CPSA shows significant advantages over the voltammetric stripping techniques.

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Section: Electrochemical Analysis In Protein-protein and Dna-protein mentioning

confidence: 99%

Constant Current Chronopotentiometric Stripping Analysis of Bioactive Peptides at Mercury and Carbon Electrodes

et al. 1998

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“…The aim of the work is (a) to test oxidation signals of aminopurines in presence of Cu(II) ions on a pencil graphite electrode of new type (PeGE); (b) to apply elimination procedure for both signals (Ox com and Ox Pur ) to enhance sensitivity and verify a charge transfer process in adsorbed state; (c) to propose the scheme of processes which take place on electrode surface; and (d) to found to what degree the complexes can be used for transfer technique (AdTSV) [32,33].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Study of Aminopurines on Pencil Graphite Electrode in the Presence of Copper Ions

et al. 2010

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Electrochemical oxidations of aminopurines (adenine, 2-aminopurine, 2,6-diaminopurine) and their complexes with Cu(I) on a pencil graphite electrode were investigated by means of linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS). The anodic process of the Cu(I)-aminopurine complex, corresponding to the oxidation of Cu(I) to Cu(II), takes place in the potential range between 0.4 and 0.5 V (vs. Ag/AgCl/3 M KCl). At more positive potentials the aminopurines provide voltammetric peaks resulting from the oxidation of the purine ring. The stability of the accumulated complex layer was investigated by the adsorptive transfer stripping technique.

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“…The adsorptive transfer stripping voltammetric (AdTSV) technique was carried out as described previously [22,32]. DNA was adsorbed at the electrode surface from 7-mL aliquots containing 0.2 M NaCl for 60 s, if not stated otherwise, without stirring.…”

Section: Methodsmentioning

confidence: 99%

Two‐Surface Strategy in Electrochemical DNA Hybridization Assays: Detection of Osmium‐Labeled Target DNA at Carbon Electrodes

et al. 2003

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Target DNAs, including a 71-mer oligonucleotide, a PCR product and a plasmid DNA, all containing oligo(A) stretches, were hybridized at magnetic Dynabeads oligo(dT) 25 (DBT). The hybridization events were detected using a technique based on chemical modification of the target DNA with a complex of osmium tetroxide with 2,2'-bipyridine (Os, bipy) and voltammetric detection at carbon electrodes. DNA was modified with Os, bipy prior to capture at DBT, at the beads, or after release from the beads. In the latter case, DNA-Os, bipy was detected in the reaction mixture using adsorptive transfer stripping voltammetry involving extraction of unreacted Os, bipy from the electrode by organic solvents. Pre-labeling of the target plasmid DNA and the PCR product with Os, bipy significantly increased the yield of DNA captured at the beads. Tens of femtomoles of both short (the 71-mer oligonucleotide) and long (the 3-kilobase plasmid) target DNAs in a 20-microliter hybridization sample can be easily detected by means of these techniques. Various carbon electrode materials, including pyrolytic graphite (PGE), highly oriented pyrolytic graphite (HOPGE), carbon paste (CPE), glassy carbon and pencil graphite, were tested regarding their suitability for the detection of osmium-labeled DNA. Among them, PGE and HOPGE appeared usable in the measurements of both purified DNA-Os, bipy and its mixtures with unreacted Os, bipy while CPE was suitable for the detection purified osmium-labeled DNA.

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Adsorptive stripping voltammetry of biomacromolecules with transfer of the adsorbed layer

Cited by 175 publications

References 7 publications

Constant Current Chronopotentiometric Stripping Analysis of Bioactive Peptides at Mercury and Carbon Electrodes

Constant Current Chronopotentiometric Stripping Analysis of Bioactive Peptides at Mercury and Carbon Electrodes

Voltammetric Study of Aminopurines on Pencil Graphite Electrode in the Presence of Copper Ions

Two‐Surface Strategy in Electrochemical DNA Hybridization Assays: Detection of Osmium‐Labeled Target DNA at Carbon Electrodes

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