Adsorption to Hydroxyapatite of Partially Deglycosylated Human Salivary Mucins in Competition with Phosvitin and Phytate

Amerongen, A. van Nieuw; Oderkerk, C.H.; Veerman, E.C.I.

doi:10.1515/bchm3.1991.372.2.585

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…On the other hand, ERICSON (30) and BRISCOE et al (31) presented evidence that removal of sialic acid from salivary glycoproteins did not change the affinity of the glycoproteins for the hydroxyapatite surface. Recently, we found that not only removal of sialic acid and fucose, but also of more internal sugars, affected the adsorption of human salivary mucins only a little or not at all (32). The aggregation and adherence of oral bacteria by pathological salivas will be studied further.…”

Section: •-mentioning

confidence: 93%

Changes in saliva of epileptic patients

Amerongen

Strooker

Oderkerk

et al. 1992

J Oral Pathology Medicine

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Unstimulated whole saliva samples of 27 indoor epileptic patients were studied on their protein composition using biochemical and immunochemical methods. A number of salivary proteins appeared at least partially to be hydrolyzed. In a number of saliva samples the concentration of carbohydrate-containing isoenzymes of amylase was reduced. In addition, the concentration of the 20 kD glycoprotein EP-GP was reduced by 60%. Sialic acid, the terminal sugar of the glycoproteins and mucins, was released for about 50% and in three salivas even nearly completely. Moreover, sialic acid- and fucose-containing epitopes could hardly be detected by monoclonal antibodies to human salivary mucins. As a consequence of this hydrolytic breakdown the saliva mediated aggregation of two S. sanguis strains had been reduced. In contrast, the aggregation of S. oralis had been maintained.

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Section: •-mentioning

confidence: 93%

Changes in saliva of epileptic patients

Amerongen

Strooker

Oderkerk

et al. 1992

J Oral Pathology Medicine

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“…Mucins have indeed been shown to be associated with calcification in different mineralizing systems: the buccal cavity, where salivary mucins strongly bind to teeth hydroxyapatite (79,80) and protect the teeth against demineralization (81); the gallbladder, where the mucin GBM promotes gallstone formation (82) but also delays the precipitation of calcium phosphate (83); and urine, where urinary mucins modulate the shape of calcium oxalates (84) and act as heterogeneous nucleants for calcium salts (85). In molluscs, mucins have been suspected of playing a role in calcification (86), but only little molecular evidence has been given (87).…”

Section: Discussionmentioning

confidence: 99%

Mucins and Molluscan Calcification

Marin¹,

Corstjens²,

Gaulejac³

et al. 2000

Journal of Biological Chemistry

183

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A cDNA expression library constructed from mantle tissue mRNA of the Mediterranean fan mussel Pinna nobilis was screened with antibodies raised against the acetic acid-soluble shell matrix of the same species. This resulted in the isolation of a 2138-base pair cDNA, containing 13 tandem repeats of 93 base pairs. The deduced protein has a molecular mass of 66.7 kDa and a isoelectric point of 4.8. This protein, which is enriched in serine and proline residues, was overexpressed, purified, and used for producing polyclonal antibodies. Immunological in situ and in vitro tests showed that the protein is localized in the nacreous aragonitic layer of P. nobilis, but not in the calcitic prisms. Because this protein of the nacre of P. nobilis exhibits some mucin-like characteristics, we propose the name mucoperlin. This is the first paper reporting the cloning of a molluscan mucin and the first molecular evidence for the involvement of a mucin in molluscan calcification. This finding corroborates our previous hypothesis that some of the proteinaceous constituents of the molluscan shell matrix would derive from mucins, common to many metazoan lineages of the late Precambrian (Marin, F., Smith, M., Isa, Y., Muyzer, G. and Westbroek, P. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 1554 -1559). The adaptation of an ancestral mucin to a new function, the regulation of the mineralization process, may be one of the molecular events, among others, that would explain the simultaneous emergence of organized calcification in many metazoan lineages during the Cambrian explosion.The skeletons produced by molluscs to protect and support their soft bodies are organo-mineral amalgamates (1, 2) exhibiting a high degree of order at the nanoscale (3). As a rule, the mineralized structure has mechanical properties far superior to each of its constituent components. A well known example is mother-of-pearl or nacre. Its micron-sized compact brick wall texture is more than 1000 times tougher than the chemically precipitated counterpart, aragonite (4).A complex biochemical machinery is required for the production of these highly ordered biominerals. Specialized microenvironments must be created to accommodate the growing minerals (1, 5). The constituent ions as well as macromolecules capable of directing and fine-tuning the crystallization must be supplied, while waste products of crystallization have to be removed and deleterious precipitations avoided. The actual biomineralization process follows as a remarkable case of selforganization, with the densely packed organic-inorganic composite emerging as the final product. Furthermore, the organisms must deploy upstream the calcification, signal transmitters (6, 7), and transcription factors (8, 9) able to switch their biomineralizing machineries on and off.Despite its complexity, it would appear as though the skeleton-forming machinery evolved with remarkable facility. The skeletons of many animal phyla were installed in less than 30 million years, since the beginning of the Cambrian, 544 million ...

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“…IP6 was reported to reduce the adsorption of native human whole salivary mucins to hydroxyapatite by 50% (Amerongen et al, 1988). However, deglycosylation of mucins increased their ability to compete with IP6 for hydroxyapatite surfaces (Amerongen et al, 1991).…”

Section: Oral Care Productsmentioning

confidence: 99%

Phytic Acid: Properties and Potential Applications in Dentistry

Nassar

Maki

et al. 2021

Front. Mater.

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Inositol hexaphosphate (IP6) is the most abundant inositol phosphate in nature and an essential molecule for different biological functions. IP6 has a unique structure granting it distinctive properties; a high negative charge density provides IP6 with an immense chelating ability and valuable antioxidant properties. IP6 is also simple and cost-effective to produce. These features have attracted researchers and entrepreneurs to further study IP6 for a wide variety of applications in areas such as pharmaceutical, food and chemical industries, medicine, pharmacy, nutrition, and dentistry. The interest in IP6 in the dental field unfolded many decades ago following identification of a cariostatic ability and a positive impact on reducing enamel dissolution. Subsequently, IP6’s anti-plaque, anti-calculus and cement-forming properties have been investigated. Despite encouraging findings, there was a phase of decreased attention to IP6 which slowed down research progress. However, the potential use of IP6 has recently been revisited through several publications that provided deeper understanding into its mechanisms of action in the aforementioned applications. Studies have also explored new applications in endodontics, adhesive, preventive and regenerative dentistry, and IP6’s role in improving the characteristics and performance of dental materials. Evidence of the merits of IP6 in dentistry is now substantial, and this narrative review presents and discusses the different applications proposed in the literature and gives insights of future use of IP6 in the fields of orthodontics, implant and pediatric dentistry.

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Adsorption to Hydroxyapatite of Partially Deglycosylated Human Salivary Mucins in Competition with Phosvitin and Phytate

Cited by 5 publications

References 14 publications

Changes in saliva of epileptic patients

Changes in saliva of epileptic patients

Mucins and Molluscan Calcification

Phytic Acid: Properties and Potential Applications in Dentistry

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