Adsorption of Single-Stranded DNA Fragments onto Cationic Aminated Latex Particles

Abstract

In this study, the adsorption of single-stranded oligodeoxyribonucleotides (ODN) onto well-characterized polymer latex particles has been investigated. At first, the case of poly(thymidylic acid) (dT35) was thoroughly examined as a function of pH and ionic strength in the presence of cationic polystyrene colloids prepared by emulsifier-free copolymerization of styrene and vinylbenzylamine hydrochloride. Due to the polyelectrolyte character of the oligodeoxyribonucleotides (which are negatively charged) and the positive charges of the particles, strong adsorption was clearly noticed together with a high affinity; in addition, a decrease in the maximal adsorption was observed upon raising the pH. These adsorption phenomena corroborate that electrostatic forces play a major role in the adsorption process; however the contribution of hydrophobic forces was also evidenced using the various adsorption isotherms at various pHs and extrapolating these results to zero surface charge density (σ = 0) and to zero zeta-potential (ζ = 0) of the latex particles. Moreover, the effect of poly(thymidylic acid) chain length on the maximum adsorbed amount on the latex particles was also investigated, providing information on the state of conformation of the ODN at the particle surface; it was suggested that the ODN adsorbs in a flat conformation. Such a behavior can be extended regardless of ODN nature so long as the support was oppositely charged. Finally, an attempt was made to predict the adsorption behavior of single-stranded ODN using the general approach of Hesselink for polyelectrolytes.

Effect of Hydrophobicity and Electrostatics on Adsorption and Surface Diffusion of DNA Oligonucleotides at Liquid/Solid Interfaces

“…On the other hand, there was no such trend in face than the hydrophobic forces. Our results agree with recent work of other groups (43)(44)(45). One must recall in RCF versus the surface density for electrostatically driven adsorption of negatively charged oligonucleotide on posiany of these generalizations, however, that all of our work was carried out at one pH and ionic strength.…”

DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes

“…Hydrophobic interactions between aromatic polymer units and DNA bases were also recently recognized (17). The free energy of polyelectrolyte͞ DNA interactions is controlled by the structure of the participating species, in conjunction with solution variables such as pH, ionic strength, and temperature (18).…”

EWS Fli-1 Antisense Nanocapsules Inhibits Ewing Sarcoma-Related Tumor in Mice

“…In general, this type of surface association was found to be rapidly reversible in the presence of protein-rich biological medium (16). Moreover, when associated through electrostatic forces, ODN adopt conformation which make them unlikely to hybridize with their biological target (17).…”