Adsorption of plasma proteins onto polymer latices

Suzawa, Toshiro; Shirahama, Hiroyuki

doi:10.1016/0001-8686(91)80021-b

Cited by 83 publications

(61 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Background due to non-specific protein binding can be a significant confounding factor in antibody-based detection. Acrylamide hydrogels can passivate the glass surface and provide lower non-specific protein adsorption than poly-L-lysine or aldehydic surfaces [36,47]. Immobilization of substrates for solid-phase assays can also present challenges.…”

Section: Discussionmentioning

confidence: 99%

A solid-phase Bcr-Abl kinase assay in 96-well hydrogel plates

Mand

Veach

et al. 2008

Analytical Biochemistry

View full text Add to dashboard Cite

Regulated phosphorylation by protein tyrosine kinases (PTKs) such as c-Abl, is critical to cellular homeostasis. In turn, once deregulated as in the Chronic Myeloid Leukemia (CML) fusion protein Bcr-Abl, PTKs can promote cancer onset and progression. The dramatic success of the Bcr-Abl inhibitor imatinib as therapy for CML has inspired interest in other PTKs as targets for cancer drug discovery. Here we report a novel PTK activity and inhibition screening method using hydrogelimmobilized peptide substrates. Using acrylate crosslinkers, we tether peptides via terminal cysteines to thiol-presenting hydrogels in 96-well plates. These surfaces display low background and high reproducibility, allowing semi-quantitative detection of peptide phosphorylation by recombinant cAbl or by Bcr-Abl activity in cell extracts using traditional anti-phosphotyrosine immunodetection and chemifluorescence. The capabilities of this assay were demonstrated by performing model screens for inhibition with several commercially available PTK inhibitors and a collection of pyridopyrimidine Src/Abl dual inhibitors. This assay provides a practical method to measure the activity of a single kinase present in a whole cell lysate with high sensitivity and specificity as a valuable means for efficient small molecule screening.

show abstract

Section: Discussionmentioning

confidence: 99%

A solid-phase Bcr-Abl kinase assay in 96-well hydrogel plates

Mand

Veach

et al. 2008

Analytical Biochemistry

View full text Add to dashboard Cite

show abstract

“…This is in line 1B adsorbed on silica at pH 5 are shown as function of the with suggestions made in the literature that IgG adsorption protein flux towards the surface. The protein flux has been preferentially takes place by its Fc part (40)(41)(42).…”

Section: Are Averages (N § 2) the Devia-mentioning

confidence: 99%

“…For IgG and F(ab ) 2 adsorption to the high charge density the two Fab parts repel each other this effect is enhanced owing to the increased distance bewhich results in a ''cigar''-shaped F(ab) 2 molecule. If some tween the two F(ab) parts (41,45). In addition, there is a of these F(ab) 2 molecules adsorb in an end-on orientation, third factor of kinetic nature.…”

Section: Are Averages (N § 2) the Devia-mentioning

confidence: 99%

Adsorption Dynamics of IgG and Its F(ab′)2and Fc Fragments Studied by Reflectometry

Buijs

Berg

Lichtenbelt

et al. 1996

Journal of Colloid and Interface Science

103

112

View full text Add to dashboard Cite

“…[23] By way of comparison, a maximum surface loading of 2.5 mg m -2 is calculated from the hydrodynamic dimensions of BSA. [26] In the final step of our process, the PLLA template is removed by solvent extraction, leaving the ultraporous polymer/protein layered structure. Partially transparent structures without deformation or cracking are obtained in the case of extraction of PLLA from sample b with methylene chloride.…”

mentioning

confidence: 99%

Ultraporous Nanosheath Materials by Layer‐by‐Layer Deposition onto Co‐continuous Polymer‐Blend Templates

2006

View full text Add to dashboard Cite

Porous polymer materials with interconnected porosity have recently received great attention due to their wide range of possible applications and the challenges they pose in preparation. Their potential applications range from use in the biomedical and pharmaceutical fields (tissue engineering and drug delivery) [1][2][3] to chromatographic materials, [4] to catalysis of chemical and biochemical reactions, [5] and to electronic devices.[6] For tissue-engineering applications, for instance, at least four different techniques for fabricating interconnected porous polymer substrates have been developed: fiber bonding of unwoven meshes, [7] solvent casting/particulate leaching, [8] gas foaming, [9] and phase separation.[10] However, every one of these approaches suffers from severe limitations including low levels of interconnectivity, low void volume, poor control of pore size and distribution, and difficulties in obtaining materials of reproducible porosities. Previously, we advanced an approach to preparing microporous materials based on the melt-blending of two immiscible polymers [11] and demonstrated that selective extraction of one of two components in a co-continuous mixture can result in a single-component structure of fully interconnected porosity with highly controlled porosity, morphology, and pore diameters ranging from 100 nm to hundreds of micrometers. [12,13] However, this method was found to be limited in the void volume that can be attained, and able to be used to prepare only relatively low-void-volume substrates (75 %). Layer-by-layer (LbL) deposition is a simple and effective approach to deposit ultrathin and uniform molecular layers onto surfaces in which polyelectrolytes are adsorbed on an oppositely charged surface, reversing the surface charge and leaving it primed for the next deposition cycle. [14][15][16] Originally exclusively investigated on macroscopically flat surfaces, this technique has been recently used to fabricate hollow spheres by assembling multilayered films onto colloidal particles and subsequently removing the core.[17] Hollow micro-and nanoparticles have also been prepared by removing the core of different crosslinked polymer brush surface-modified particles. [18,19] Macromolecular self-assembly techniques such as block-copolymer templates have already been used to prepare porous materials, [20,21] but to date no work has been reported on the use of LbL techniques to create 3D fully interconnected substrates.Here we report the use of LbL deposition onto a porous co-continuous blend template as a novel route towards ultrahigh-void-volume materials of high internal surface and closely controlled pore size. As an illustrative case, we selected bovine serum albumin (BSA) as a bioactive compound to be incorporated in the protocol. The present process is shown in Figure 1.First, co-continuous poly(L-lactic acid)/poly(e-caprolactone) (PLLA/PCL 50:50 wt.-%) blends are produced via melt-processing. In order to demonstrate the preparation of materials of different pore sizes, two pre...

show abstract

Adsorption of plasma proteins onto polymer latices

Cited by 83 publications

References 58 publications

A solid-phase Bcr-Abl kinase assay in 96-well hydrogel plates

A solid-phase Bcr-Abl kinase assay in 96-well hydrogel plates

Adsorption Dynamics of IgG and Its F(ab′)2and Fc Fragments Studied by Reflectometry

Ultraporous Nanosheath Materials by Layer‐by‐Layer Deposition onto Co‐continuous Polymer‐Blend Templates

Contact Info

Product

Resources

About