Adsorption of Olive Leaf (<i>Olea europaea</i> L.) Antioxidants on Silk Fibroin

Bayçın, Deniz; Altıok, Evren; Ülkü, Semra; Bayraktar, Oğuz

doi:10.1021/jf062829o

Cited by 80 publications

(43 citation statements)

References 32 publications

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“…In our previous study batch isotherms have shown that silk fibroin is efficient adsorbent for purification of polyphenols from olive leaf extract [34]. To explore the adsorption capacity during continuous operation, a laboratory mini column filled with silk fibroin was used.…”

Section: Adsorption and Desorption Of Olive Leaf Antioxidants By Usinmentioning

confidence: 99%

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Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin

Altıok¹,

Bayçın²,

Bayraktar³

et al. 2008

Separation and Purification Technology

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163

110

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General objective of the present work was to assess the isolation of polyphenols from olive leaves. The effects of extraction conditions on the total phenol content and antioxidant activity of olive leaf extract (OLE) were investigated. An extract with good antioxidant activity (7.52 mmol of Trolox equivalent antioxidant capacity (TEAC)/g olive leaf extract), and a high content of oleuropein (13.4%) and rutin (0.18%) could be obtained using 70% ethanol as extraction solvent. There was a good correlation between the antioxidant activity and the total phenol content. Furthermore, silk fibroin was used as a novel adsorbent to recover the polyphenols from the olive leaf extracts. The adsorbed amounts of rutin and oleuropein were 15 mg rutin/g silk fibroin and 96 mg oleuropein/g silk fibroin. Fraction consisting of mainly oleuropein and fraction rich in rutin, luteolin-7-glucoside, verbascoside, apigenin-7-glucoside were obtained by using silk fibroin filled column. Silk fibroin was found to be a promising adsorbent for the purification of oleuropein and rutin from olive leaf extracts.

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Section: Adsorption and Desorption Of Olive Leaf Antioxidants By Usinmentioning

confidence: 99%

“…Its hydrophobic character makes it favorable adsorbent used in adsorption studies [33]. Because of its promising health effects and bounding mechanisms, silk fibroin can be considered as a potent adsorbent for the isolation of antioxidants from the crude olive leaf extracts [34].…”

Section: Introductionmentioning

confidence: 99%

Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin

Altıok¹,

Bayçın²,

Bayraktar³

et al. 2008

Separation and Purification Technology

Self Cite

163

110

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“…Silkworm silk is an important natural fiber and has extensive application in the textile industry, due to its characteristic luster, moisture absorbance, and mechanical strength. The application of silkworm silk has also been extended from traditional textile industry to bionic material, biological medicine, cosmetics, food, and other fields …”

Section: Introductionmentioning

confidence: 99%

Low pressure‐induced secondary structure transitions of regenerated silk fibroin in its wet film studied by time‐resolved infrared spectroscopy

Liu

Zhou

et al. 2018

Proteins

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The secondary structure transitions of regenerated silk fibroin (RSF) under different external perturbations have been studied extensively, except for pressure. In this work, time-resolved infrared spectroscopy with the attenuated total reflectance (ATR) accessory was employed to follow the secondary structure transitions of RSF in its wet film under low pressure. It has been found that pressure alone is favorable only to the formation of β-sheet structure. Under constant pressure there is an optimum amount of D O in the wet film (D O : film = 2:1) so as to provide the optimal condition for the reorganization of the secondary structure and to have the largest formation of β-sheet structure. Under constant amount of D O and constant pressure, the secondary structure transitions of RSF in its wet film can be divided into three stages along with time. In the first stage, random coil, α-helix, and β-turn were quickly transformed into β-sheet. In the second stage, random coil and β-turn were relatively slowly transformed into β-sheet and α-helix, and the content of α-helix was recovered to the value prior to the application of pressure. In the third and final stage, no measurable changes can be found for each secondary structure. This study may be helpful to understand the secondary structure changes of silk fibroin in silkworm's glands under hydrostatic pressure.

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“…While some of the phenolic components of OLE degraded into polar compounds, some of them may have chosen to strongly bond to fibroin molecules. This is because hydrophobic phenolic components prefer to bond to protein molecules by hydrophobic interactions in an aqueous medium (Tang et al, ; Baycin et al, ). Flavones that have retention times close to oleuropein may be interacted with SF selectively.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Sheath/Core nanofibers containing olive leaf extract

Doğan

Başal

Bayraktar

et al. 2015

Microsc. Res. Tech.

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This study aimed at producing silk fibroin (SF)/hyaluronic acid (HA) and olive leaf extract (OLE) nanofibers with sheath/core morphology by coaxial electrospinning method, determining their antimicrobial properties, and examining release profiles of OLE from these coaxial nanofibers. Optimum electrospinning process and solution parameters were determined to obtain uniform and bead-free coaxial nanofibers. Scanning electron microscopy and transmission electron microscopy (TEM) were used to characterize the morphology of the nanofibers. The antimicrobial activities of nanofibers were tested according to AATCC test method 100. Total phenolic content and total antioxidant activity were tested using in vitro batch release system. The quality and quantity of released components of OLE were determined by high-performance liquid chromatography. The changes in nanofibers were examined by Fourier-transform infrared spectroscopy. Uniform and bead-free nanofibers were produced successfully. TEM images confirmed the coaxial structure. OLE-loaded nanofibers demonstrated almost perfect antibacterial activities against both of gram-negative and gram-positive bacteria. Antifungal activity against C. albicans was rather poor. After a release period of 1 month, it was observed that $70-95% of the OLE was released from nanofibers and it was still bioactive. Overall results indicate that the resultant shell/core nanofibers have a great potential to be used as biomaterials. Microsc.

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Adsorption of Olive Leaf (Olea europaea L.) Antioxidants on Silk Fibroin

Cited by 80 publications

References 32 publications

Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin

Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin

Low pressure‐induced secondary structure transitions of regenerated silk fibroin in its wet film studied by time‐resolved infrared spectroscopy

Bioactive Sheath/Core nanofibers containing olive leaf extract

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