Adsorption of oligopeptides on silver nanoparticles: surface‐enhanced Raman scattering and theoretical studies

Garrido, C.; Aliaga, Álvaro; Gómez-Jeria, Juan S.; Clavijo, R. E.; Campos‐Vallette, Marcelo; Sánchez-Cortés, Santiago

doi:10.1002/jrs.2583

Cited by 26 publications

(12 citation statements)

References 36 publications

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“…In fact, the bands at 848 and 1150 cm −1 , and the asymmetric one at 1577 cm −1 , could contain information on the presence tyrosine (Tyr) modes following Garrido et al . The weak band at 1451 cm −1 , the medium band at 1268 cm −1 and one band of the double band at 1150 cm −1 are ascribed to vibrational modes of the serine amino acid; the two first bands could also contain information of the CH 2 deformation of the alanine residue. Another CH 2 deformation of alanine is observed only in SERS at 670 cm −1 .…”

Section: Resultsmentioning

confidence: 99%

Raman and surface enhanced Raman scattering of a black dyed silk

Aguayo

Garrido

Clavijo

et al. 2013

J Raman Spectroscopy

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Artículo de publicación ISIThe Raman and surface enhanced Raman scattering (SERS) spectra of a black dyed silk sample (BDS) were registered. The spectral analysis was performed on the basis of Raman and SERS spectral data of isolated samples of Bombyx mori silk fibroin, its motif peptide component (GAGAGS) and the synthetic reactive black 5 dye (RB5). The macro FT-Raman spectrum of the silk sample is consistent with a silk II-Cp crystalline fraction of Bombyx mori silk fibroin; the SERS spectrum is highly consistent with conformational modifications of the fibroin due to the interactions with the Ag nanoparticles. The GAGAGS peptide sequence dominates the Raman spectrum of the silk. The SERS spectrum of the peptide suggests a random coil conformation imposed by the surface interaction; the serine residue in the new conformation is exposed to the surface. Quantum chemical calculations for a model of the GAGAGS–Ag surface predict a nearly extended conformation at the Ag surface. The Raman spectrum of the dye was analysed, and a complete band assignment was proposed; it was not possible to propose a preferential orientation or organization of the molecule on the metal surface. Quantum chemical calculations for a model of the dye interacting with a silver surface predict a rather coplanar orientation of the RB5 on the Ag metal surface. The Raman spectrum of the BDS sample is dominated by signals from the dye; the general spectral behaviour indicates that the dye mainly interacts with the silk through the sulphone (–SO2–) and sulphonate (–SO2–O–) groups. Besides the presence of dye signals, mainly ascribed to the sulphone and sulphonate bands, the SERS spectrum of the BDS sample also displays bands belonging to the amino acids alanine, glycine, serine and particularly tyrosine

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Section: Resultsmentioning

confidence: 99%

Raman and surface enhanced Raman scattering of a black dyed silk

Aguayo

Garrido

Clavijo

et al. 2013

J Raman Spectroscopy

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“…In previous works with peptides and proteins, we have evidenced the importance of the Lys and Arg residues on the interaction with metal surfaces . In particular, we have demonstrated the inductive effect of the guanidinium moiety on the orientation and interaction of complex biological systems with Ag nanoparticles (AgNps).…”

Section: Introductionmentioning

confidence: 86%

The effect of the pH on the interaction of L‐arginine with colloidal silver nanoparticles. A Raman and SERS study

Garrido

Aguayo

Clavijo

et al. 2013

J Raman Spectroscopy

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Artículo de publicación ISIRaman and surface enhanced Raman scattering (SERS) spectroscopies were used to study the pH effect (7 to 9) on the interaction of arginine (Arg) with colloidal Ag nanoparticles (AgNps). A new methodology was implemented in order to obtain reproducible SERS spectra in solution. The dependence of the Arg concentration on the stability of the AgNps is discussed. A pH increasing of the colloidal solution to the limits of the Arg pKa2 value induces a preferential and stable Arg–metal interaction. j potential measurements of the Arg–AgNps system at different pH conditions studied provide information about the Arg–AgNps interaction; the pH increasing favors the interaction. SERS spectra at pH 7 indicate that the molecule interacts with the Ag surface only through the guanidinium fragment. By increasing the pH to 9, the molecule adopts a new conformation on the surface; the metal–analyte interaction is verified through the guanidinium, carboxylate and the aliphatic moieties. In addition, theoretical calculations performed by using the extended Hückel method for a model of Arg interacting with an Ag surface support the observed SERS results

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“…of a range of naturally occurring carotenoids in over 50 specimens of plant tissue have been undertaken, and the characteristic bands of C=C and C–C stretching and C–CH bending have been recorded . The MRKDV peptide structurally associated with an immunomodulatory protein and model peptides ADEDRDA and LGRGISL with common amino acid residues were studied by Garrido and coworkers using SERS supported by quantum chemistry computations . Theoretical calculations performed by using extended Huckel theory and 6‐31G* methods for a model of arginine interacting with a silver cluster supported the observed experimental results.…”

Section: Biosciencesmentioning

confidence: 99%