Adsorption of lysozyme to phospholipid and meibomian lipid monolayer films

Mudgil, Poonam; Torres, Margaux; Millar, Thomas J.

doi:10.1016/j.colsurfb.2006.01.017

Cited by 69 publications

(61 citation statements)

References 30 publications

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“…For the purpose we formed mixed pseudo-binary films of MGS and polar lipid, Egg Sphingomyelin (SM) or Dipalmitoylphosphatidylcholine (DPPC), at the air/water interface of Langmuir surface balance. Langmuir surface balance was already successfully employed in pioneer studies of the interactions between tear proteins and meibium [18][19][20][21][22][23]. SM and DPPC are chosen because they were used in previous studies as synthetic mimics of TF polar lipids [24,25].…”

Section: Introductionmentioning

confidence: 99%

Interactions of Meibomian gland secretion with polar lipids in Langmuir monolayers

Georgiev

Kutsarova

Jordanova

et al. 2010

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tThe surface interactions of Meibomian gland secretion (MGS) with polar lipid (PL), Egg Sphingomyelin (SM) or Dipalmitoylphosphatidylcholine (DPPC), are studied in mixed pseudo-binary films formed at the air/water interface of Langmuir surface balance. The behavior of the mixed films during slow quasi-equilibrium compression and during fast dynamic compression-decompression is registered by measurements of surface pressure and surface potential, and by monitoring film morphology with Brewster Angle Microscopy (BAM). Quasi-equilibrium compression isotherms are used to calculate the excess Gibbs and Helmholtz energy of mixing between MGS and PLs and thus to evaluate the interactions between the lipid compounds at the interface. The effects of PLs on the mixed film's elastic moduli of area compressibility, morphology and capability to attain high surface pressures are also examined.PLs interact with MGS with different strength and in different manner: MGS-SM interaction is weak and might lead to interfacial disaggregation of the thick meibium domains when SM is in excess, while MGS-DPPC interaction is strong and results in the formation of thick lipid aggregates. Both PLs increase the mixed films reciprocal compressibility and capability to achieve higher surface pressures. The results demonstrate that in vitro studies of the surface interactions between MGS and PLs might be beneficial in the selection of PLs for artificial tear formulations and for examination on molecular scale of the possible role of PLs at the ocular surface.

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Section: Introductionmentioning

confidence: 99%

Interactions of Meibomian gland secretion with polar lipids in Langmuir monolayers

Georgiev

Kutsarova

Jordanova

et al. 2010

Colloids and Surfaces B: Biointerfaces

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“…Combining (23) and (25) is not easily accomplished, particularly for general values of α; the ability to combine these easily and get analytical expressions for t was crucial for the success of the Newtonian case studied by Howell.…”

Section: Shock Formationmentioning

confidence: 98%

“…Other interfacial properties, such as the apparent surface tension of the tear-air interface, are definitely affected by polar lipids that are attracted to the lipid-aqueous interface [19,20], and those lipids can cause upward motion of the tear fluid after a blink [21,22]. However, the apparent surface tension of tears appears to be affected by more than the presence of polar lipids [23,24].…”

Section: Introductionmentioning

confidence: 99%

Thin film dynamics on a prolate spheroid with application to the cornea

et al. 2011

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The tear film on the front of the eye is critical to proper eyesight; in many mathematical models of the tear film, the tear film is assumed to be on a flat substrate. We re-examine this assumption by studying the effect of a substrate which is representative of the human cornea. We study the flow of a thin fluid film on a prolate spheroid which is a good approximation to the shape of the human cornea. Two lubrication models for the dynamics of the film are studied in prolate spheroidal coordinates which are appropriate for this situation. One is a self-consistent leading-order hyperbolic partial differential equation (PDE) valid for relatively large substrate curvature; the other retains the next higher-order terms resulting in a fourth-order parabolic PDE for the film dynamics. The former is studied for both Newtonian and Ellis (shear thinning) fluids; for typical tear film parameter values, the shear thinning is too small to be significant in this model. For larger shear thinning, we find a significant effect on finite-time singularities. The second model is studied for a Newtonian fluid and allows for a meniscus at one end of the domain. We do not find a strong effect on the thinning rate at the center of the cornea. We conclude that the corneal shape does not have a significant effect on the thinning rate of the tear film for typical conditions.

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“…The film formation at the water/air interface (Langmuir film technique) has been applied to study the behaviour of Meibomian lipids [19][20][21][22][23] and especially to study the interaction of them with lachrymal proteins [24][25][26][27]. The Langmuir technique has been applied previously by the author to study the behaviour of lipid and phospholipid films [28,29].…”

Section: Introductionmentioning

confidence: 99%

Langmuir films study on lipid-containing artificial tears

Torrent‐Burgués

2016

Colloids and Surfaces B: Biointerfaces

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Lipid-containing artificial tears are a type of artificial tears that use lipid components in order to restore the lipid layer of the tear film. One of these components is lecithin which can be applied in spray solutions containing lecithin liposomes. In this work the behavior of three of these commercial tears based on lecithin, Innoxa, Opticalm and Optrex, are studied using the Langmuir technique. The obtained isotherms are presented, discussed and compared. This technique seems useful in order to see the film behavior of the lipid components of these tears and determine some important parameters such as fluidity and extension at the air-water interface, and allows us to discern differences between these commercial tears. Innoxa and Optrex tears are more similar to each other than to Opticalm tears. Opticalm presents more fluidity, probably due to the presence of more insaturations in the fatty acid chains of the phospholipids of the lecithin used in fabrication.

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Adsorption of lysozyme to phospholipid and meibomian lipid monolayer films

Cited by 69 publications

References 30 publications

Interactions of Meibomian gland secretion with polar lipids in Langmuir monolayers

Interactions of Meibomian gland secretion with polar lipids in Langmuir monolayers

Thin film dynamics on a prolate spheroid with application to the cornea

Langmuir films study on lipid-containing artificial tears

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