Adsorption of Human Reagins to Ethylchloroformate Polymerized Antigen E and Desorption with Thiocyanate Ions

Tannenbaum, Marion; Goodfriend, L.

doi:10.1159/000230568

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…Recently, M alley et al [10] employed human albumin to stabilize reaginic activity re covered from immunosorbent by elution with 6 M urea. On the other hand, even though stabilizing proteins were not employed in the experi ments reported by T annenbaum and G oodfriend, these investigators claimed a high recovery of reagins on their elution from the appropriate immunosorbent with buffers containing thiocyanate ions [14].…”

Section: Discussionmentioning

confidence: 99%

Isolation of Human Reagins Differing in Their Affinity for Ragweed Immunosorbent

Kisil¹,

Attallah²,

Sehon³

et al. 1974

Int Arch Allergy Immunol

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Reaginic antibodies to ragweed pollen which had been adsorbed to cellulose-allergen immunosorbents were eluted in the presence of homologous or heterologous serum protein(s) at a concentration of 3–7 mg/ml with gly-HCl at pH 2.5, or 6 m urea at pH 7.4. These extraneous serum proteins exerted a stabilizing effect on the eluted reagins. Two groups of reagins differing in their affinity for the allergen(s) were sequentially eluted from the immunosorbent with gly-HCl and Nal regardless of the order of addition of these eluting agents. Moreover, specific elution of some reagins was achieved with a low molecular weight, hapten-like preparation of ragweed pollen.

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Section: Discussionmentioning

confidence: 99%

Isolation of Human Reagins Differing in Their Affinity for Ragweed Immunosorbent

Kisil¹,

Attallah²,

Sehon³

et al. 1974

Int Arch Allergy Immunol

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Biospecific Affinity Chromatography and Related Methods

Porath¹,

Kristiansen²

1975

The Proteins

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Diagnosis of Multiple Inhalant Allergies in Children by Radioimmunoassay

Hoffman

Haddad

1974

Pediatrics

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A series of 302 patients reporting immediate hypersensitivity symptoms to inhalant allergens was evaluated by both puncture skin testing and the radioallergosorbent test (RAST) using 13 common inhalant allergens. Agreement between the two tests varied from 71% to 93% for the various allergens and averaged 82%. The best correlation, 93%, was obtained with mixed grass pollen allergens. If a purified allergen, rye group I, was used the correlation rose to 97.5%. The correlation was 88% for the mold Alternaria, and the negative-sera did not become positive when tested over a wide range of dilutions. Fifty-eight percent of the patients with house dust allergy reacted to house dust mite (Dermatophagoides farinae). Correlations of 71% to 93% were obtained with ten weed and tree pollens. The only patients in whom positive RASTs were found with negative skin tests to the same antigen had either severe atopic dermatitis or decreased skin response to exogenous histamine. Sera from a group of 20 patients with positive allergy history but negative skin tests also had negative RAST. The radioallergosorbent test appears to add little information to that obtained by a comprehensive allergy history and puncture skin tests in the great majority of children with inhalant allergy. The only exceptions were children with severe atopic dermatitis, suppressed skin reactivity or dermatographism.

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Adsorption of Human Reagins to Ethylchloroformate Polymerized Antigen E and Desorption with Thiocyanate Ions

Cited by 3 publications

References 7 publications

Isolation of Human Reagins Differing in Their Affinity for Ragweed Immunosorbent

Isolation of Human Reagins Differing in Their Affinity for Ragweed Immunosorbent

Biospecific Affinity Chromatography and Related Methods

Diagnosis of Multiple Inhalant Allergies in Children by Radioimmunoassay

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