Adsorption of fibronectin and vitronectin onto Primaria™ and tissue culture polystyrene and relationship to the mechanism of initial attachment of human vein endothelial cells and BHK-21 fibroblasts

Steele, John G.

doi:10.1016/0142-9612(95)98901-p

Cited by 150 publications

(108 citation statements)

References 42 publications

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“…The ability of Vn to outcompete other serum components in the race for the surface is well documented, 11,[33][34][35] as is the influence of surface chemistry on this process. 20,28,36 For many cultured cell types, the adsorption of Vn from serum enables their initial attachment. 28,37 It is not surprising, then, that removal of this active molecule from serum largely abolished cell adhesion and spreading on both DMS and EDS regions.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, Fn is a far less competitive molecule, 33-37 so it does not adsorb from serum in sufficient amounts to promote cell adhesion, as observed on a variety of other substrates. 28,[36][37][38] Its removal from serum therefore had little effect on attachment. In common with other substrates, 37,38 EDS and DMS chemistries precoated with Fn supported cell attachment, implying that the molecule retained its adhesive activity, but was effectively absent from the surface in competitive situations.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Protein adsorption and cell attachment to patterned surfaces

McFarland

Thomas

DeFilippis

et al. 2000

J. Biomed. Mater. Res.

142

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To better understand the events involved in the generation of defined tissue architectures on biomaterials, we have examined the mechanism of attachment of human bone-derived cells (HBDC) to surfaces with patterned surface chemistry in vitro. Photolithography was used to generate alternating domains of N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane (EDS) and dimethyldichlorosilane (DMS). At 90 min after seeding, HBDC were localized preferentially to the EDS regions of the pattern. Using sera specifically depleted of adhesive glycoproteins, this spatial organization was found to be mediated by adsorption of vitronectin (Vn) from serum onto the EDS domains. In contrast, fibronectin (Fn) was unable to adsorb in the face of competition from other serum components. These results were confirmed by immunostaining, which also revealed that both Vn and Fn were able to adsorb to EDS and DMS regions when coated from pure solution, i.e., in the absence of competition. In this situation, each protein was able to mediate cell adhesion across a range of surface densities. Cell spreading was constrained on the EDS domains, as indicated by cell morphology and the lack of integrin receptor clustering and focal adhesion formation. This spatial constraint may have implications for the subsequent expression of differentiated function.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Protein adsorption and cell attachment to patterned surfaces

McFarland

Thomas

DeFilippis

et al. 2000

J. Biomed. Mater. Res.

142

View full text Add to dashboard Cite

show abstract

“…In other words, these three gels can be classified into neutral gels with lower protein adsorption, negatively charged hydrophilic gels with low protein adsorption, and negatively charged hydrophobic gels with high protein adsorption as the ability of substrates to adsorb such proteins from serum containing culture medium determines their ability to support cell adhesion and spreading. 53,54 Hence EBs behaviors may be differed from each other because of the variance of protein adsorptions onto these surfaces in this study.…”

Section: Protein-surface Interactionsmentioning

confidence: 99%

Dynamic Behavior and Spontaneous Differentiation of Mouse Embryoid Bodies on Hydrogel Substrates of Different Surface Charge and Chemical Structures

Liu

Chen

Yang

et al. 2011

Tissue Engineering Part A

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Differentiation of embryoid bodies (EBs) into particular cell lineages has been extensively studied. There is an increasing interest in the effect of soft hydrogel scaffolds on the behavior of EBs, such as the initial adhesion, dynamic morphology change, and differentiation. In this study, without adding any other bioactive factors in the serum-containing medium, dynamic behaviors of mouse EBs loaded on the surface of hydrogels with different surface charge and chemical structures are investigated. EBs adhered quickly to negatively charged poly(sodium p-styrene sulfonate) (PNaSS) hydrogels, which facilitates EBs spreading, migration, and differentiation into three germ layers with high efficiency of cardiomyocytes differentiation, similar to that on gelatin coated polystyrene (PS) culture plate. While on neutral poly(acrylamide) (PAAm) hydrogels, EBs maintained the initial spherical morphology with high expression of pluripotency-related markers in the short culture periods, and then showed the significantly greater levels of selected endoderm markers after long-time culture. EBs cultured on negatively charged poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS) gels demonstrated the analogous behaviors with that of neutral PAAm gels at early differentiation phase (day 4 + 1). Then, their adhesion, spreading and differentiation were quite similar to that on negatively charged PNaSS gels. The correlation between surface properties of hydrogels and EBs differentiation was discussed.

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“…The reaction of cells on implant surfaces is on one hand cell type dependent [19] and on the other hand largely determined by the protein layer covering the implant surface [20]. Latter protein layer is to some degree dependent from the surface characteristics [21] but mainly determined by the composition of the surrounding¯uid [22]. Latter layer also plays an important role in in vitro studies.…”

Section: Discussionmentioning

confidence: 99%