Adsorption of enzymes to stimuli-responsive polymer brushes: Influence of brush conformation on adsorbed amount and biocatalytic activity

Koenig, Meike; Bittrich, Eva; König, Ulla; Rajeev, B.; Müller, Martin; Eichhorn, Klaus‐Jochen; Thomas, Sabu; Stamm, Manfred; Uhlmann, Petra

doi:10.1016/j.colsurfb.2016.07.015

Cited by 32 publications

(47 citation statements)

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“…The HNs with plentiful carboxyl groups were considered specifically advisable for this method, as the steric hindrance was no longer dominant in this process. Physical adsorption [44][45][46] could enable the 5-AF to enter the inside of the hairy nanospheres easily, and their positions in the polyelectrolyte could be rearranged. Therefore, a large amount of 5-AF could be embedded in the PAA structure by the electrostatic driving force.…”

Section: Effect Of Coupling Methodsmentioning

confidence: 99%

Hairy Fluorescent Nanospheres Based on Polyelectrolyte Brush for Highly Sensitive Determination of Cu(II)

Wang

Chen

et al. 2020

Polymers

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Currently, it is an ongoing challenge to develop fluorescent nanosphere detectors that are uniform, non-toxic, stable and bearing a large number of functional groups on the surface for further applications in a variety of fields. Here, we have synthesized hairy nanospheres (HNs) with different particle sizes and a content range of carboxyl groups from 4 mmol/g to 9 mmol/g. Based on this, hairy fluorescent nanospheres (HFNs) were prepared by the traditional coupling method (TCM) or adsorption-induced coupling method (ACM). By comparison, it was found that high brightness HFNs are fabricated based on HNs with poly (acrylic acid) brushes on the surface via ACM. The fluorescence intensity of hairy fluorescent nanospheres could be controlled by tuning the content of 5-aminofluorescein (5-AF) or the carboxyl groups of HNs easily. The carboxyl content of the HFNs could be as high as 8 mmol/g for further applications. The obtained HFNs are used for the detection of heavy metal ions in environmental pollution. Among various other metal ions, the response to Cu (II) is more obvious. We demonstrated that HFNs can serve as a selective probe and for the separation and determination of Cu(II) ions with a linear range of 0–0.5 μM and a low detection limit of 64 nM.

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Section: Effect Of Coupling Methodsmentioning

confidence: 99%

Hairy Fluorescent Nanospheres Based on Polyelectrolyte Brush for Highly Sensitive Determination of Cu(II)

Wang

Chen

et al. 2020

Polymers

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“…When the enzyme is adhered to hydrophobic interfaces, the "lid" changes to "open" conformation, enabling the access of the substrate to the active site and enhancing thereby the lipase activity dramatically, up to 50% of the activity of that shown by the native lipase (Palomo et al, 2002). There are many other examples of enzymes immobilized through adsorption on polymeric materials such as laccase (Labus et al, 2012), glucose oxidase (Koenig et al, 2016), carbonic anhydrase (Assarsson et al, 2014), and cellulase (Wang S. et al, 2013). Furthermore, there are interesting biopolymers for adsorbing enzymes, such as chitosan, calcium alginate, cellulose, agarose, or commercially available ion-exchange resins.…”

Section: Physical Adsorptionmentioning

confidence: 99%

Tunable Polymeric Scaffolds for Enzyme Immobilization

Rodriguez‐Abetxuko

Sánchez-deAlcázar

Muñumer

et al. 2020

Front. Bioeng. Biotechnol.

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The number of methodologies for the immobilization of enzymes using polymeric supports is continuously growing due to the developments in the fields of biotechnology, polymer chemistry, and nanotechnology in the last years. Despite being excellent catalysts, enzymes are very sensitive molecules and can undergo denaturation beyond their natural environment. For overcoming this issue, polymer chemistry offers a wealth of opportunities for the successful combination of enzymes with versatile natural or synthetic polymers. The fabrication of functional, stable, and robust biocatalytic hybrid materials (nanoparticles, capsules, hydrogels, or films) has been proven advantageous for several applications such as biomedicine, organic synthesis, biosensing, and bioremediation. In this review, supported with recent examples of enzyme-protein hybrids, we provide an overview of the methods used to combine both macromolecules, as well as the future directions and the main challenges that are currently being tackled in this field.

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“…This feature allows for the easy recovery and reuse of the polymer . Additionally, some polyelectrolyte brushes have been developed using poly(2‐vinylpyridine) and mixtures with methacrylic acid, which change their conformation in response to changes in the pH of the medium . Like thermoresponsive polymers, pH‐responsive polymers also have reversible phase transition properties causing solubility and insolubility as the response to the medium changes .…”

Section: Polymer Supports For Degradation Of Organic Pollutantsmentioning

confidence: 99%

“…110,111 Additionally, some polyelectrolyte brushes have been developed using poly(2-vinylpyridine) and mixtures with methacrylic acid, which change their conformation in response to changes in the pH of the medium. 112 Like thermoresponsive polymers, pH-responsive polymers also have reversible phase transition properties causing solubility and insolubility as the response to the medium changes. 109 In general, the immobilization on these polymers generates good properties in the immobilized enzymes, eliminates diffusional problems, allows their reuse after enzyme deactivation, and preserves a high activity in the enzyme.…”

Section: Crosslinkingmentioning

confidence: 99%

Polymer supports for the removal and degradation of hazardous organic pollutants: an overview

Urbano

Bustamante

Palacio

et al. 2020

Polymer International

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Over the last decades, the presence of highly organic pollutants has increased and become an environmental problem that affects all forms of life. To solve or reduce this problem, multiple strategies have been proposed for the elimination and degradation of organic compounds in aqueous media. This review aims to revise and critically discuss the most recent advances in polymer supports to be used for the adsorption and degradation of organic pollutants. However, the greatest challenge with respect to this issue is the industrial scale‐up of bioremediation processes that allow the removal and degradation of compounds in a continuous and large‐scale manner. © 2019 Society of Chemical Industry

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Adsorption of enzymes to stimuli-responsive polymer brushes: Influence of brush conformation on adsorbed amount and biocatalytic activity

Cited by 32 publications

References 50 publications

Hairy Fluorescent Nanospheres Based on Polyelectrolyte Brush for Highly Sensitive Determination of Cu(II)

Hairy Fluorescent Nanospheres Based on Polyelectrolyte Brush for Highly Sensitive Determination of Cu(II)

Tunable Polymeric Scaffolds for Enzyme Immobilization

Polymer supports for the removal and degradation of hazardous organic pollutants: an overview

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