Adsorption of Endotoxin on Polymyxin B Immobilized Affinity Matrices

Tang, Shouwan; Kong, Liang; Ou, Junjie; Zou, Hanfa

doi:10.1016/s1872-2040(06)60024-8

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…To date, many LPS affinity ligands have been reported, histamine, histidine (His) [9], polymyxin B [10], dimethylamine [11] and polyethyleneimine [12]. Among these ligands, His is most widely used due to its stability, innocuity and afford ability and has been immobilized on Sepharose-4B or nylon membrane for LPS removal.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and endotoxin removal properties of a novel affinity sorbent with poly(1-vinylimidazole) as the ligand

Zhang

Ping

et al. 2011

Process Biochemistry

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Section: Introductionmentioning

confidence: 99%

Synthesis and endotoxin removal properties of a novel affinity sorbent with poly(1-vinylimidazole) as the ligand

Zhang

Ping

et al. 2011

Process Biochemistry

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“…For wild‐type tobacco extracts, we used Polymyxin B agarose to remove the low levels of adventitious endotoxins that reflect the colonization of the plants by bacteria. The endotoxin level in wild‐type extracts (∼37 EU mg −1 HCP) was reduced to 2.0 EU mg −1 HCP after passing the extract through Polymyxin B agarose (Fig.…”

Section: Resultsmentioning

confidence: 99%

Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins

Arfi

Hellwig

Drossard

et al. 2016

Biotechnology Journal

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The production of biopharmaceutical proteins in plants requires efficient downstream processing steps that remove impurities such as host cell proteins (HCPs) and adventitious endotoxins produced by bacteria during transient expression. We therefore strived to develop effective routines for endotoxin removal from plant extracts and the subsequent use of the extracts to generate antibodies detecting a broad set of HCPs. At first, we depleted the superabundant protein ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) for which PEG precipitation achieved the best results, preventing a dominant immune reaction against this protein. We found that a mixture of sera from rabbits immunized with pre-depleted or post-depleted extracts detected more HCPs than the individual sera used alone. We also developed a powerful endotoxin removal procedure using Polymyxin B for extracts from wild type plants or a combination of fiber-flow filtration and EndoTrap Blue for tobacco plants infiltrated with Agrobacterium tumefaciens. The antibodies we generated will be useful for quality and performance assessment in future process development and the methods we present can easily be transferred to other expression systems rendering them useful in the field of plant molecular farming.

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“…Gels comprising either physical or chemical cross‐links can undergo controlled and reversible shape changes in response to an applied field [25,26] . The optimization of these smart properties has opened many doors for applications in shape‐memory materials, artificial organs in biotechnology, microfluidics, drug delivery, and lab‐on‐chip applications, [27–34] The most common stimuli‐responsive studies are based on temperature, pH, and electric field; all these studies contributed enormously to the field of advanced functional materials [35–38] …”

Section: Introductionmentioning

confidence: 99%

Peripherally Modified Poly(amido amine) Nanocomposite Hydrogel with Stimuli‐Responsive Self‐Healing, High Tensile Strength, and Selective Superadsorption Poperties

Bharathan Jeneena,

Vivek

2023

ChemistrySelect

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Smart materials with dual functionalities demonstrate considerable potential in various applications. Designing and synthesizing hydrogels with excellent stimuli‐responsive self‐healing and high mechanical strength is extremely challenging. The present study initiates the development of a new generation of smart materials with selective superadsorption capacity. Here, we introduce a novel anthracene‐modified poly (amido amine) (PAMAM)‐N‐isopropyl acrylamide hydrogel (PAMNG). The hydrogel becomes hybrid as we incorporate the silica nanoparticle. PAMAM dendrimer‐based organic/inorganic hybrid silica nanoparticles (PAMAGN) have been characterized using nuclear magnetic resonance (NMR), Fourier‐transform infrared spectroscopy (FT‐IR), scanning electron microscopy (SEM), transmission electrone microscopy (TEM) and rheology. The developed hybrid gel exhibited excellent near‐infrared (IR) responsive self‐healing character. The hybrid gel had excellent mechanical strength, which was as high as 106 Pa. Further, the gel was used for superadsorption studies and showed selective adsorption of methyl orange from water. The adsorption was 96 %, which is remarkable. The results taken together suggest that the PMNG gel can be a promising candidate for developing multifunctional smart materials. In the future, we aim to explore how this self‐healing mechanism applies to biological applications.

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Adsorption of Endotoxin on Polymyxin B Immobilized Affinity Matrices

Cited by 8 publications

References 9 publications

Synthesis and endotoxin removal properties of a novel affinity sorbent with poly(1-vinylimidazole) as the ligand

Synthesis and endotoxin removal properties of a novel affinity sorbent with poly(1-vinylimidazole) as the ligand

Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins

Peripherally Modified Poly(amido amine) Nanocomposite Hydrogel with Stimuli‐Responsive Self‐Healing, High Tensile Strength, and Selective Superadsorption Poperties

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