Adsorption of DNA Nitrogenous Bases on Nanodiamond Particles: Theory and Experiment

Abstract

Efficiency of adsorption of nucleic acid nitrogenous bases (NBs) on carboxylated detonation nanodiamond (DND−COOH) particles in aqueous media at pH = 7.4−7.6 and pH = 13.4 was investigated using Raman spectroscopy and infrared (IR) absorption spectroscopy. A significant difference in the adsorption activity of NDs toward four different individual NBs, guanine, adenine, cytosine, and thymine, had been observed. The highest adsorption activity on DND−COOH was observed for cytosine and, in descending order, for adenine and thymine. At the same time, adsorption activities of the adenine−thymine and guanine−cytosine complementary pairs on nanodiamonds (NDs) were similar. Analysis of the hydrogen bond parameters in the adsorption of complementary pair adenine−thymine on the ND surface had been done using the density functional theory-based molecular modeling. The theoretical calculations are consistent with the experimental results.

Bilayer Adsorption of Lysozyme on Nanodiamonds in Aqueous Suspensions

“…It is known that when DNDs are suspended in water, the surface carboxyl groups of nanoparticles actively interact with water molecules, as a result of which hydrogen bonds in water are weakened. ,,, This effect can be observed by the changes in characteristics of the stretching band of the Raman scattering of water in DND suspensions.…”

“…Nanodiamonds are good adsorbents of various substances. − The corresponding functionalization of the ND surface provides it with high selective adsorption activity for heavy metal ions, nitrates, , proteins and DNA molecules, − drugs, , viruses, etc. It was shown by many authors that metal ions, nitrates, proteins, e.g., cytochrome c, or many drugs such as doxorubicin are adsorbed on the surface of nanodiamonds through physical adsorption.…”

Pharmacodynamic Studies of Fluorescent Diamond Carriers of Doxorubicin in Liver Cancer Cells and Colorectal Cancer Organoids

“…PDT colon organoids 18SH112T (colorectal cancer organoids) were maintained in culture as described previously. 59 , 60 CRC organoids were grown in complete organoid media, consisting of Advanced DMEM/F12 (GIBCO), supplemented with 1X B27 (GIBCO), 1X N2 (GIBCO), 100 µg/mL Primocin (InvivoGen), 50 ng/μL recombinant human EGF (Peprotech, NJ, USA), 10 nM Gastrin (Sigma Inc.), 500 nM A83-01 (Tocris Bioscience, Bristol, UK), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 10 mM nicotinamide (Sigma Inc.), 20% Noggin conditioned media, 10% R-Spondin 1 conditioned media and 10% Wnt3A conditioned media.…”

The energy of hydrogen bonds in aqueous suspensions of nanodiamonds with different surface functionalization

“…For numerical simulations, we use a split‐valence basis set 6‐31G(d). For numerical modeling of intermolecular forces in molecular compound, we used the Gaussian 09 program complex, which is traditionally applied for investigations in various areas of computational physics and chemistry and approved by the authors and in other investigations.…”