Adsorption of Complement Protein C3 at Polymer Surfaces

Deposition of colloid particles at heterogeneous and patterned surfaces

“…A number of interesting experimental data has also been gathered using the AFM observations for latex [21,24,26], colloid gold [81] or dendrimer suspensions [32]. One can also apply the electron microscopy to determine particle coverage [18] or the indirect methods like reflectometry [15,22,32,37], ellipsometry [82], radioisotopic labelling [83][84][85], or streaming potential [40,41,[86][87][88]. The latter method is especially sensitive and can be used for particles of arbitrary size ranging from ions, polyelectrolytes, proteins to large colloid particles of micrometer size range [89].…”

The effect of phosphorylcholine‐coated materials on the inflammatory response and fibrous capsule formation: In vitro and in vivo observations

“…Indeed, this effect has been reported previously by Tang and Eaton, 12 who showed that PET preincubated with albumin passivated the surface preventing very few neutrophils or phagocytes adhering compared with the uncoated PET to which large numbers of phagocytes were attracted. Moreover, studies by Malmsten et al 13 have shown that preadsorbed complement pro- tein C3 on various surfaces can be competitively displaced and hence passivated by HS albumin, which may further explain why the use of C3-depleted HS showed little difference in cell binding compared with the HS with C3. Given sufficient time it may be that these proteins would be displaced by the Vroman effect, 14 leading to more thermodynamically favorable and irreversible interaction with other proteins such as fibrinogen or high-molecular-weight kininogen, which are responsible for mediating inflammatory cell adhesion.…”

Real-Time Monitoring of Peptide–Surface and Peptide–Antibody Interaction by Means of Reflectometry and Surface Plasmon Resonance

“…Therefore, reflec-(18). Conformational change as a result of adsorption onto tometry may find its most powerful application in the coma hydrophobic surface, a phenomenon often reported for parison of relative data on mass and activity on a surface proteins (15,19), could also be a determining factor in the similar to the polymer surface generally used in immunodipeptide adsorption process. agnostics.…”