Adsorption of BSA on Highly Carboxylated Microspheres—Quantitative Effects of Surface Functional Groups and Interaction Forces

Yoon, Jeong Yeol; Park, Ham Yong; Kim, Jung Hyun; Kim, Woo Sik

doi:10.1006/jcis.1996.0075

Cited by 154 publications

(116 citation statements)

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“…Such significant generation of signal intensity is a direct function of presence of surface -COOH groups. Among different forces that control the interaction between proteins and supporting surfaces, hydrogen binding and hydrophobic interaction are the most dominant ones (Yoon et al 1996). Yoon et al in an extensive research work concluded that between these two major forces, hydrogen binding can perform in a stronger manner than hydrophobic interaction.…”

Section: Resultsmentioning

confidence: 99%

“…Apart from hydrophobic interaction, available surface -COOH groups in PHB fibers which are coated by comp. (9:1) provide the second chance for proteins to be accommodated on the surface in an even more substantial manner, hydrogen binding (Yoon et al 1996). In that sense, the question might be raised regarding relatively lower performances of PHB fibers coated with other two compositions, comp.…”

Section: Resultsmentioning

confidence: 99%

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Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors

Hosseini

Azari

Farahmand

et al. 2015

Biosensors and Bioelectronics

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors

Hosseini

Azari

Farahmand

et al. 2015

Biosensors and Bioelectronics

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“…In the present case, polymer-coated PHBV fibers create the platform for the mentioned physical Among several different forces that have an impact on biomolecular interfaces, three main interactions are known as the most influential driving forces in analyte-surface binding. The forces that have frequently been reported in the literature for their considerable impact on protein immobilization are as follows: electrostatic (ionic) attraction, hydrophobic interaction and hydrogen (H) bonding [24,36,37]. The ionic attraction is sensitive to pH changes and is relatively weak in comparison to other two forces while H-bonding facilitates the most substantial protein attachment [36].…”

Section: Morphology Analysis Of the Coated And Uncoated Poly(3-hydroxmentioning

confidence: 99%

“…The forces that have frequently been reported in the literature for their considerable impact on protein immobilization are as follows: electrostatic (ionic) attraction, hydrophobic interaction and hydrogen (H) bonding [24,36,37]. The ionic attraction is sensitive to pH changes and is relatively weak in comparison to other two forces while H-bonding facilitates the most substantial protein attachment [36]. In the present case, polymer-coated PHBV fibers create the platform for the mentioned physical Among several different forces that have an impact on biomolecular interfaces, three main interactions are known as the most influential driving forces in analyte-surface binding.…”

Section: Morphology Analysis Of the Coated And Uncoated Poly(3-hydroxmentioning

confidence: 99%

Intrant ELISA: A Novel Approach to Fabrication of Electrospun Fiber Mat-Assisted Biosensor Platforms and Their Integration within Standard Analytical Well Plates

et al. 2016

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A combination of far-field electrospinning (FFES) and free-radical polymerization has been used to fabricate coated electrospun polymer fiber mats as a new type of biosensor platform. Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) electrospun fibers were dip-coated with different compositions of poly methyl methacrylate-co-methacrylic acid (poly(MMA-co-MAA)). This synergistic approach utilizes large specific surface area of PHBV fibers and co-polymer coatings that feature an optimum concentration of surface carboxyl (-COOH) groups. The platform surface morphology, porosity and tunable hydrophobicity enhance biomolecular interactions via plurality of molecular forces. These customized fiber mats have been integrated into a newly designed 96-well plate called an "intrant enzyme-linked immunosorbent assay" or i-ELISA. I-ELISA allows colorimetric sandwich assay to be carried out without any modifications or additional steps in ELISA methodology. By introducing the fiber mats in fabrication of i-ELISA via extensions on the lid, we address some of the limitations of the previous designs while demonstrating an enhanced signal intensity up to 12 times higher than that of conventional assays. With improved sensitivity, specificity and accuracy in the detection of dengue virus, i-ELISA has proven to be a reliable platform for biomolecular recognition. The proposed fiber mat-assisted well plate in this study holds great potential as a universal approach for integration of different types of fiber mats with pre-designed specific properties in order to enhance the detection sensitivity of the assay.

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“…Because of its major significance, numerous experimental works have been devoted to quantify protein, in particular Fb adsorption on various substrates using the solution depletion methods (Ramsden 1993), the gravimetric methods, especially the quartz microbalance (QCM) (Reisch et al 2009;Choi et al 2002), optical methods such as ellipsometry and reflectometry (Melmsten 1994;Buijs et al 1996Buijs et al , 1997, fluorescence methods such as the total internal fluorescence (TIRF) (Yoon et al 1996;Wertz and Santore 2001) and isotope labeling (Zembala and Dejardin 1994;Zembala et al 1998). For the low coverage range the electron microscopy and the AFM methods can be quite efficient in a direct determination of protein coverage (Hall and Slayter 1959;Veklich et al 1993;Ortega-Vinuesa et al 1998;Sit 1999;Marchin and Berrie 2003;Toscano and Santore 2006).…”

Section: Introductionmentioning

confidence: 99%

Irreversible adsorption of latex particles on fibrinogen covered mica

2010

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Physicochemical properties of bovine plasma fibrinogen (Fb) in electrolyte solutions were characterized. These comprised the diffusion coefficient (hydrodynamic radius), determined by the DLS method, electrophoretic mobility and the isoelectric point. The hydrodynamic radius of Fb was 12 nm for pH < 5. The number of uncompensated (electrokinetic) charges on the protein N c was calculated from the electrophoretic mobility data. It was found that for pH < 5.8 the electrokinetic charge was positive, independently of the ionic strength and negative for pH > 5.8. For pH = 3.5 the value of N c , was 26 for 10 −3 M. Similar electrokinetic measurements were performed for the mica substrate using the streaming potential cell. It was shown that for pH = 3.5 and 10 −3 M, the zeta potential of mica remained negative (−50 mV). This promoted an irreversible, electrostatically driven adsorption of Fb, which was confirmed in experiments carried out under diffusion-controlled transport. The surface concentration of Fb on mica was determined directly by AFM counting. By adjusting the time of adsorption, Fb monolayers of desired coverage were produced. Independently, the presence of Fb on mica was determined quantitatively by the colloid enhancement method, in which negatively charged latex particles were used, having the diameter of 800 nm. It was found that for Fb coverage below 0.05 the method was more sensitive than other indirect methods. The experimental data obtained in latex deposition experiments were adequately interpreted in terms of the random site model used previously for polyelectrolytes. It was shown that adsorption sites consisted of a cluster of Z. Adamczyk ( ) · M. Nattich · M. Wasilewska

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Adsorption of BSA on Highly Carboxylated Microspheres—Quantitative Effects of Surface Functional Groups and Interaction Forces

Cited by 154 publications

References 0 publications

Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors

Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors

Intrant ELISA: A Novel Approach to Fabrication of Electrospun Fiber Mat-Assisted Biosensor Platforms and Their Integration within Standard Analytical Well Plates

Irreversible adsorption of latex particles on fibrinogen covered mica

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