Adsorption of bovine serum albumin onto styrene/2-hydroxyethyl methacrylate copolymer latex

Shirahama, Hiroyuki; Suzawa, Toshiro

doi:10.1016/0021-9797(85)90050-5

Cited by 67 publications

(30 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The adsorbability of BSA onto ferrite beads prepared by the reference method showed a similar tendency to that onto polystyrene latex reported by Shirahama and Suzawa, 14 with the exception of the value at pH 3.0. Beads prepared by method M-1 showed a higher amount of bound BSA over the whole range of pH values measured than did beads prepared by the two other methods.…”

Section: Amount Of Bound Bsasupporting

confidence: 61%

Effects of Microwave Irradiation on Nonspecific Protein Binding in the Solid Phase Coated with Bovine Serum Albumin

Kawaguchi

Higo

2005

Polym J

View full text Add to dashboard Cite

ABSTRACT:In solid-phase analytical techniques, a reduction in nonspecific binding at the solid-phase surface is key to obtaining excellent assay performance. We have developed a new preparation method to minimize nonspecific protein binding in the solid phase. Microwave irradiation (2.45 GHz, 500 W) was used to produce a highly protein-resistant, dense coating layer of bovine serum albumin (BSA) that was acid-denatured at pH 4.0. The protein resistance in the BSA-coated magnetic bead was assessed by measuring the enzyme activity of alkaline phosphatase-labeled mouse immunoglobulin G (ALP-IgG) that nonspecifically bound to the surface. In addition, we observed the BSA-coated beads by atomic force microscopy (AFM) and also quantitatively measured the bound BSA, enabling us to propose a model for the denaturation processes caused by microwave irradiation. Compared to conventional BSA-coated beads prepared at pH 8.0 without microwave treatment, the activity of nonspecifically bound ALP-IgG was markedly reduced to 3%. Our results strongly suggest that a meshwork film of extensively associated BSA molecules is formed on the bead surface. This new technique should be useful for the development of polymer supports for use in solid-phase separation analyses, such as heterogeneous immunosorbent assay and column chromatography.

show abstract

Section: Amount Of Bound Bsasupporting

confidence: 61%

Effects of Microwave Irradiation on Nonspecific Protein Binding in the Solid Phase Coated with Bovine Serum Albumin

Kawaguchi

Higo

2005

Polym J

View full text Add to dashboard Cite

show abstract

“…The decrease in -( is proportional to the content of HEMA in copolymers. 25 These results are attributed to the structure of the electrical double layer of these latices. 25 On the other hand, the (-potential of the polymer microspheres produced by EAP-501 was nearly zero (O~ -9) because the zwitterionic EAP-501 fragment was localized on the surface of the particles.…”

Section: (-Potential Measurements Of Particlesmentioning

confidence: 85%

“…25 These results are attributed to the structure of the electrical double layer of these latices. 25 On the other hand, the (-potential of the polymer microspheres produced by EAP-501 was nearly zero (O~ -9) because the zwitterionic EAP-501 fragment was localized on the surface of the particles. The surfaces of the polymer microspheres in the emulsion were thus drawn based on the results of XPS and (-potential measurements as shown in Figure 6.…”

Section: (-Potential Measurements Of Particlesmentioning

confidence: 85%

Preparation and Characterization of Polystyrene Microspheres Surface-Modified with Glucoside Moieties

Sugiyama

Oku

1995

Polym J

View full text Add to dashboard Cite

ABSTRACT:The emulsifier-free emulsion copolymerization of 2-(glucosyloxy) ethyl methacrylate (GEMA) and styrene (St), yielded a series of copolymer microspheres [poly(GEMA-coSt) microspheres] with varying content of the GEMA moiety. 2-[(2-Ethylphosphatoethyl)dimethylammonio Jethyl 4,4'-azobis(4-cyanovalerate) (EAP-501) and potassium persulfate (KPS) were used as initiators. From the copolymerization of GEMA and St it was found that the initial rate of polymerization of St increased in the presence of a small amount of GEMA. Poly(GEMA-co-St) microspheres showed unimodal distribution of particle sizes with 240--440 nm, which depended on mo!% of GEMA to St in feed and species of initiator. The (-potential of the particles was nearly zero (0--9mV) and -68--99mV for poly(GEMA-co-St) microspheres produced by EAP-501 and KPS, respectively. From XPS measurements the GEMA moiety was found located on the surface of the particles. Poly(GEMA-co-St) microspheres with a hydrophilic surface suppressed the adsorption of albumin (Alb) and globulin (Glo), less than the poly(St) microspheres as the control. It was concluded that the suppression of adsorption of a protein onto poly(GEMA-co-St) microspheres is dependent on hydrophilicity due to the GEMA moiety and ammonium phosphate group derived from EAP-501 on the surface of particles.KEY WORDS Emulsion Copolymerization / Glucoside Monomer / Surface-Modified Polystyrene Microsphere / XPS / Albumin / Globulin / Adsorption /

show abstract

“…The magnitudes of protein concentrations used in the biotechnological applications usually fall in the range of the proposed method. [42][43][44][45] The precise determination of PTT involves the use of a visible spectrophotometer equipped with a heating system and a thermometer capable of measuring the cell temperature with an accuracy of 0.1°C. Alternatively, the detection of PTT can be roughly made by using a simple thermometer and by following the turbidity of the copolymer-albumin solution visible to the naked eye.…”

Section: Albumin-copolymer Interactionmentioning

confidence: 99%

A novel approach for albumin determination in aqueous media by using temperature‐ and pH‐sensitive N‐isopropylacrylamide‐co‐N‐[3‐(dimethylamino)‐propyl]methacrylamide random copolymers

Tuncel

Demirgöz

Patir

et al. 2002

J of Applied Polymer Sci

View full text Add to dashboard Cite

Random copolymers of N-isopropylacrylamide (NIPA) and N- [3-(dimethylamino)propyl]methacrylamide (DMAPM) were synthesized by solution polymerization using azobisizobutyronitrile as the initiator in 1,4-dioxane at 60°C. NIPA-co-DMAPM copolymer exhibited both temperature and pH sensitivity. Thermally reversible phase transitions were observed both in the acidic and the alkaline pH regions for copolymers produced with different DMAPM/NIPA feed ratios. The pH dependency of the lower critical solution temperature (LCST) was stronger for copolymers produced with higher DMAPM feed concentrations. NIPA-co-DMAPM random copolymer was also sensitive to the albumin concentration. In the presence of albumin, thermally irreversible phase transitions were observed in slightly acidic and neutral media. However, reversible transitions were obtained in aqueous media containing albumin at basic pH. The phase-transition temperature of NIPA-co-DMAPM copolymer significantly decreased with increasing albumin concentration at both acidic and alkaline pH values. This behavior was explained by albumin binding onto the copolymer chains by means of H-bond formation between the dimethylamino groups of the copolymer and the carboxyl groups of albumin. For a certain range of albumin concentration, the phase-transition temperature exhibited a linear decrease with increasing albumin concentration. By utilizing this behavior, a simple albumin assay was developed. The results indicated that NIPA-co-DMAPM copolymer could be utilized as a new reagent for the determination of albumin concentration in the aqueous medium. The proposed method was valid for the albumin concentration range of 0-4000 g/mL. The protein concentrations commonly utilized in biotechnological studies fall in the range of the proposed method.

show abstract

Adsorption of bovine serum albumin onto styrene/2-hydroxyethyl methacrylate copolymer latex

Cited by 67 publications

References 13 publications

Effects of Microwave Irradiation on Nonspecific Protein Binding in the Solid Phase Coated with Bovine Serum Albumin

Effects of Microwave Irradiation on Nonspecific Protein Binding in the Solid Phase Coated with Bovine Serum Albumin

Preparation and Characterization of Polystyrene Microspheres Surface-Modified with Glucoside Moieties

A novel approach for albumin determination in aqueous media by using temperature‐ and pH‐sensitive N‐isopropylacrylamide‐co‐N‐[3‐(dimethylamino)‐propyl]methacrylamide random copolymers

Contact Info

Product

Resources

About