Adsorption of Biomineralization Protein Mms6 on Magnetite (Fe3O4) Nanoparticles

Arai, Kosuke; Murata, Satoshi; Wang, Taifeng; Yoshimura, Wataru; Oda-Tokuhisa, Mayumi; Matsunaga, Tadashi; Kisailus, David; Arakaki, Atsushi

doi:10.3390/ijms23105554

Cited by 9 publications

(6 citation statements)

References 57 publications

(73 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with the presence of a transmembrane region, Mms6 has been identified in enriched magnetosome membranes (13). The MIC is a region of acidic amino acids that binds ferrous iron (18,34), ferric iron (17,35,36), magnetite crystal (37,38), and other minerals (17). The MIC has been implicated in iron crystal nucleation (17,18) and protein localization of Mms6 (23).…”

Section: Resultsmentioning

confidence: 99%

Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles inMagnetospirillum magneticumAMB-1

Bickley,

Komeili

2024

Preprint

View full text Add to dashboard Cite

Magnetotactic bacteria are a diverse group of microbes that use magnetic particles housed within intracellular lipid-bounded magnetosome organelles to guide navigation along geomagnetic fields. Development of magnetosomes and their magnetic crystals inMagnetospirillum magneticumAMB-1 requires the coordinated action of numerous proteins. Most proteins are thought to localize to magnetosomes during the initial stages of organelle biogenesis regardless of environmental conditions. However, magnetite-shaping protein Mms6 is only found in magnetosomes that contain magnetic particles, suggesting that it might conditionally localize after the formation of magnetosome membranes. The mechanisms for this unusual mode of localization to magnetosomes are unclear. Here, using pulse-chase labeling, we show that Mms6 translated under non-biomineralization conditions translocates to pre-formed magnetosomes when cells are shifted to biomineralizing conditions. Genes essential for magnetite production, namelymamE, mamM,andmamOare necessary for Mms6 localization, whereasmamNinhibits Mms6 localization. MamD localization is also investigated and found to be controlled by similar cellular factors. The membrane localization of Mms6 is dependent on a glycine-leucine repeat region, while the N-terminal domain of Mms6 is necessary for retention in the cytosol and impacts conditional localization to magnetosomes. The N-terminal domain is also sufficient to impart conditional magnetosome localization to MmsF, altering its native constitutive magnetosome localization. Our work illuminates an alternative mode of protein localization to magnetosomes in which Mms6 and MamD are kept in the cytosol by MamN until biomineralization initiates, whereupon they translocate into magnetosome membranes to control the development of growing magnetite crystals.ImportanceMagnetotactic bacteria (MTB) are a diverse group of bacteria that form magnetic nanoparticles surrounded by membranous organelles. MTB are widespread and serve as a model for bacterial organelle formation and biomineralization. Magnetosomes require a specific cohort of proteins to enable magnetite formation, but how those proteins are localized to magnetosome membranes is unclear. Here, we investigate protein localization using pulse-chase microscopy and find a system of protein coordination dependent on biomineralization permissible conditions. In addition, our findings highlight a protein domain that alters the localization behavior of magnetosome proteins. Utilization of this protein domain may provide a synthetic route for conditional functionalization of magnetosomes for biotechnological applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles inMagnetospirillum magneticumAMB-1

Bickley,

Komeili

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…( 2011 ) overexpressed Bfr in EcN and found that Bfr expression could be monitored by MRI for changes in contrast. Mms6 is a magnetosome membrane protein with iron binding activity (Arai et al., 2022 ). Yavuz et al.…”

Section: Bacteria Imaging In Tumour Diagnosismentioning

confidence: 99%

Bacterial imaging in tumour diagnosis

Chu,

Yu,

Jiang

et al. 2024

Microbial Biotechnology

View full text Add to dashboard Cite

Some bacteria, such as Escherichia coli (E. coli) and Salmonella typhimurium (S. typhimurium), have an inherent ability to locate solid tumours, making them a versatile platform that can be combined with other tools to improve the tumour diagnosis and treatment. In anti‐cancer therapy, bacteria function by carrying drugs directly or expressing exogenous therapeutic genes. The application of bacterial imaging in tumour diagnosis, a novel and promising research area, can indeed provide dynamic and real‐time monitoring in both pre‐treatment assessment and post‐treatment detection. Different imaging techniques, including optical technology, acoustic imaging, magnetic resonance imaging (MRI) and nuclear medicine imaging, allow us to observe and track tumour‐associated bacteria. Optical imaging, including bioluminescence and fluorescence, provides high‐sensitivity and high‐resolution imaging. Acoustic imaging is a real‐time and non‐invasive imaging technique with good penetration depth and spatial resolution. MRI provides high spatial resolution and radiation‐free imaging. Nuclear medicine imaging, including positron emission tomography (PET) and single photon emission computed tomography (SPECT) can provide information on the distribution and dynamics of bacterial population. Moreover, strategies of synthetic biology modification and nanomaterial engineering modification can improve the viability and localization ability of bacteria while maintaining their autonomy and vitality, thus aiding the visualization of gut bacteria. However, there are some challenges, such as the relatively low bacterial abundance and heterogeneously distribution within the tumour, the high dimensionality of spatial datasets and the limitations of imaging labeling tools. In summary, with the continuous development of imaging technology and nanotechnology, it is expected to further make in‐depth study on tumour‐associated bacteria and develop new bacterial imaging methods for tumour diagnosis.

show abstract

“…In the case of Magnetospirillum, magnetite-binding proteins Mms5, Mms6, Mms7 (MamD), MmsF, and Mms13 (MamC) are responsible for promoting crystal growth and maintaining their shapes. 24,46,[91][92][93][94][95][96][97] Factors for magnetosome biosynthesis outside of MAI…”

Section: Roles Of the Conserved Magnetosome-associated Proteins In Maimentioning

confidence: 99%

“…This step contains no core magnetosome proteins, indicating that biomineralization processes of magnetite or greigite crystals are performed via species‐ or phylum‐specific proteins may contribute to species‐specific characteristics of the shape, size, and numbers of crystals. In the case of Magnetospirillum , magnetite‐binding proteins Mms5, Mms6, Mms7 (MamD), MmsF, and Mms13 (MamC) are responsible for promoting crystal growth and maintaining their shapes 24,46,91–97 …”

Section: Introductionmentioning

confidence: 99%

Recent advances in studies on magnetosome‐associated proteins composing the bacterial geomagnetic sensor organelle

Taoka

Eguchi

Shimoshige

et al. 2023

Microbiology and Immunology

View full text Add to dashboard Cite

Magnetotactic bacteria (MTB) generate a membrane‐enclosed subcellular compartment called magnetosome, which contains a biomineralized magnetite or greigite crystal, an inner membrane–derived lipid bilayer membrane, and a set of specifically targeted associated proteins. Magnetosomes are formed by a group of magnetosome‐associated proteins encoded in a genomic region called magnetosome island. Magnetosomes are then arranged in a linear chain–like positioning, and the resulting magnetic dipole of the chain functions as a geomagnetic sensor for magneto‐aerotaxis motility. Recent metagenomic analyses of environmental specimens shed light on the sizable phylogenetical diversity of uncultured MTB at the phylum level. These findings have led to a better understanding of the diversity and conservation of magnetosome‐associated proteins. This review provides an overview of magnetosomes and magnetosome‐associated proteins and introduces recent topics about this fascinating magnetic bacterial organelle.

show abstract

Adsorption of Biomineralization Protein Mms6 on Magnetite (Fe3O4) Nanoparticles

Cited by 9 publications

References 57 publications

Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles inMagnetospirillum magneticumAMB-1

Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles inMagnetospirillum magneticumAMB-1

Bacterial imaging in tumour diagnosis

Recent advances in studies on magnetosome‐associated proteins composing the bacterial geomagnetic sensor organelle

Contact Info

Product

Resources

About