Adsorption mechanisms of RNA mononucleotides on silver nanoparticles

Miljanić, Snežana; Dijanošić, Adriana; Matić, Ivona

doi:10.1016/j.saa.2014.09.040

Cited by 26 publications

(24 citation statements)

References 24 publications

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“…For example, Raman signatures of the nucleic acids comprising DNA are well characterized both experimentally (Dijanošić et al 2014;Hobro et al 2013;Kuzmin et al 2013;Miljanić et al 2015) and theoretically (Arivazhagan et al 2014). Specifically, the DNA Raman spectrum exhibits both ribose ( $ 900 cm À 1 ) and several high frequency nucleotide modes located in regions free from strong vibrational components associated with the VLP-NC, BC-NC, and mica Raman modes (e.g., 1500-1600 cm À 1 ), facilitating rapid identification of DNA-related bands during the SERS studies.…”

Section: Resultsmentioning

confidence: 99%

A virus-based nanoplasmonic structure as a surface-enhanced Raman biosensor

Lebedev

Griva

Dressick

et al. 2016

Biosensors and Bioelectronics

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Fabrication of nanoscale structures with localized surface plasmons allows for substantial increase in sensitivity of chem/bio sensors. The main challenge for realizing complex nanoplasmonic structures in solution is the high level of precision required at the nanoscale to position metal nanoparticles in 3D. In this study, we report a virus-like particle (VLP) for building a 3D plasmonic nanostructure in solution in which gold nanoparticles are precisely positioned on the VLP by directed self-assembly techniques. These structures allow for concentration of electromagnetic fields in the desired locations between the gold nanoparticles or "hot spots". We measure the efficiency of the optical field spatial concentration for the first time, which results in a ten-fold enhancement of the capsid Raman peaks. Our experimental results agree with our 3D finite element simulations. Furthermore, we demonstrate as a proof-of-principle that the plasmonic nanostructures can be utilized in DNA detection down to 0.25 ng/μl (lowest concentration tested), while the protein peaks from the interior of the nanoplasmonic structures, potentially, can serve as an internal tracer for the biosensors.

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Section: Resultsmentioning

confidence: 99%

A virus-based nanoplasmonic structure as a surface-enhanced Raman biosensor

Lebedev

Griva

Dressick

et al. 2016

Biosensors and Bioelectronics

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“…Besides, the adsorption mechanisms of some mononucleotides (AMP, GMP, CMP) on silver nanoparticles have been recently studied by surface-enhanced Raman scattering [57]. For purine mononucleotides, AMP and GMP, adsorption onto the silver nanoparticles through the six-membered ring of the nitrogenous base was suggested.…”

Section: Resultsmentioning

confidence: 99%

Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

Muntean

Leopold

Tripon

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…(ii) The adsorption mechanisms of the RNA mononucleotides onto the citrate coated silver nanoparticles had been thoroughly studied and discussed in our previous work. [16] (iii) Since the mononucleotides are the building blocks of the nucleic acids, knowing the Raman scattering enhancement of the structural elements attached to the various SERS substrate would be beneficial in the future SERS measurements of biomacromolecules.…”

Section: Sers Enhancementmentioning

confidence: 99%

“…Based on a detailed analysis of the SERS spectra, adsorption mechanisms of the RNA mononucleotides on the silver nanoparticles had been thoroughly studied in our previous work and were discussed elsewhere. [16] While selecting the appropriate band for the EF calculation, a distinctive, intense and isolated band was preferred and as such observed in both, the Raman and SERS spectrum. Even though slightly overlapped by neighboring bands, particularly in the case of the guanine mononucleotide, the Raman bands at 728, 669, 782 and 783 cm 1 , assigned to the aromatic ring breathing, were chosen for evaluating the Raman scattering enhancement of AMP, GMP, CMP and UMP, respectively.…”

Section: Sers Enhancementmentioning

confidence: 99%

“…The adsorption of the RNA mononucleotides onto the citrate coated silver nanoparticles was studied in our previous work. 16 The SERS spectra, obtained using NIR excitation, implied orientation of the molecules on the silver nanoparticles with the nucleic bases faced towards the metal surface, as sterically and electrostatically most convenient. Moreover, for the purine mononucleotides, AMP and GMP, adsorption through the larger aromatic ring was suggested, which was concentration dependent in case of AMP.…”

Section: Sers Enhancementmentioning

confidence: 99%

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Surface-enhanced Raman Scattering Enhancement Factors for RNA Mononucleotides on Silver Nanoparticles

Miljanić¹,

Ratkaj²,

Avdejev³

et al. 2015

Croat. Chem. Acta

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Surface-enhanced Raman scattering (SERS) enhancement factors (EF) were evaluated for RNA mononucleotides: adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP), cytidine 5'-monophosphate (CMP) and uridine 5'-monophosphate (UMP), on silver nanoparticles, which differed in shape (nanospheres, nanostars) and stabilizing anionic layer (chlorides, citrates) on the metal surface. In freshly prepared silver colloids the enhanced Raman scattering was observed for all the RNA mononucleotides on the chloride coated silver nanospheres, Ag_Cl nsp (EF ≈ 10 4 ), for AMP only on the citrate coated silver nanospheres, Ag_cit nsp (EF ≈ 10 3 ), while not obtained at all for any of the mononucleotides on the citrate stabilized silver nanostars, Ag_cit nst. Upon aggregation, the SERS activity of all the silver colloids increased, whereby the purine mononucleotides, AMP and GMP, more strongly scattered radiation on Ag_Cl nsp, and the pyrimidine mononucleotides, CMP and UMP, on Ag_cit nsp. Regardless of the silver nanoparticles, the higher EFs were evaluated for AMP and GMP (EF up to 5 × 10 6 ), than for CMP and UMP (EF ≈ 5 × 10 4 ).

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Adsorption mechanisms of RNA mononucleotides on silver nanoparticles

Cited by 26 publications

References 24 publications

A virus-based nanoplasmonic structure as a surface-enhanced Raman biosensor

A virus-based nanoplasmonic structure as a surface-enhanced Raman biosensor

Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

Surface-enhanced Raman Scattering Enhancement Factors for RNA Mononucleotides on Silver Nanoparticles

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