“…To determine the adsorbed amounts of copolymers and protein on polylactide surface, FTIR spectroscopy with attenuated total reflectance (ATR) optics was employed. Although, this technique is less frequently used for this purpose, compared with ellipsometry [29][30][31][32], reflectometry [33], surface plasmon resonance [34,35], or radiolabeling technique [19,23], it has been well established for quantification of protein adsorption [36,37] and was reported to be as sensitive as most radiolabeling techniques [38]. In addition, compared to the other mentioned techniques, ATR-FTIR spectroscopy can provide additional useful information on surface layers related to chemical structure, molecular orientation [39] or, in the case of protein layers, secondary structure of adsorbed molecules [40].…”