Adsorption, immobilization, and hybridization of DNA studied by the use of quartz crystal oscillators

Yamaguchi, Shuichi; Shimomura, Takeshi; Tatsuma, Tetsu; Oyama, Noboru

doi:10.1021/ac00062a019

Cited by 140 publications

(59 citation statements)

References 5 publications

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“…The parallel resonant frequency depends not only on the quartz crystal but also on the associated circuit and all capacitances in parallel with the device (eq. [14], Table 3). The measurement o f f , can be very useful in determining specific properties of the h o u n d i n g media such as in the case of electrolytes (26).…”

Section: Conductivity (D'nil)mentioning

confidence: 99%

“…These entities, bearing one of the reacting pairs, are attracted to the sensor surface by a magnetic field. Recently, Oyama and co-workers (14) reported that A-phage DNA can be covalently immobilized onto the gold surface of a TSM sensor and that dissociation and hybridization of immobilized DNA can be monitored by impedance analysis in the gas phase. The occurrence of immobilization and hybridization of DNA were then said to be confirmed by a small decrease off,,,,, (the frequency giving the maximum conductance) and increases of motional resistance.…”

Section: Introductionmentioning

confidence: 99%

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Rheological and interfacial properties of nucleic acid films studied by thickness – shear mode sensor and network analysis

Su¹,

Thompson²

1996

Can. J. Chem.

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Rheological and interfacial properties of nucleic acid films studied by thickness -shear mode sensor and network analysisAbstract: Complete characterization of the thickness -shear mode (TSM) acoustic wave sensor coated with films of nucleic acid and subjected to conductive solution loading is achieved through network analysis of impedance measurements. The responses of coated sensors with respect to series resonant frequency can be separated into two regions. When 0 < u < 0.5 R-I m-' the frequencies for sensors with films of single strand (ss) DNA or polycytidylic (5') (Poly C) in place rise significantly with increasing conductivity. The motional resistances for sensors coated with both species peak at the same value of conductivity where the frequency has the most rapid shift. In the second region (0.5 < u < 12 R-' m-I), the frequency response for the ss DNA system exhibits a bell-shaped curve, with a minimum at u = 4 0-' m-l. The motional resistances for both nucleic acid layers show reversible behaviour, whereas TSM sensors with bare PdO and Au electrodes yield little change. Models that consider only the bulk properties of solutions fail to explain the observed changes in frequency. On the other hand, an equivalent circuit model incorporating the rheological properties of nucleic acid layers on the sensor surface is successful in explaining the various responses.Key words: DNA sensor, network analysis, thickness -shear mode sensor, DNA interfacial properties, RCsumC : Faisant appel i une analyse de rCseau des mesures d3impCdance, on a rkalisC une caracthrisation complkte de senseurs enduits de films d'acide nuclkique, opCrant en mode de cisaillement de I'Cpaisseur et soumis h un chargement de solution conductrice. Les rkponses des senseurs enduits par rapport h la frkquence de rCsonance en sCrie ont pu &tre sCparCe en deux rkgions. Lorsque 0 < a < 0,5 m-I, les frequences des senseurs avec des films d'ADN i simples brins (sb) ou polycytidyliques (5') (Poly C) en place augmentent beaucoup avec une augmentation de la conductivitC. Les rCsistances au mouvement des senseurs enduits avec les deux espkces atteignent un sommet pour la m&me valeur de la conductivitk alors que la frCquence prCsente le dkplacement le plus rapide. Dans la deuxikme region (0,5 < u < 12 m-I), la frkquence de rCponse du systkme ADN sb prCsente une courbe en forme de cloche avec un minimum i u = 4 R-I m-I. Les rCsistances au mouvement pour les deux couches d'acides nuclCiques prksentent dans les deux cas un comportement reversible alors que le senseur MCE avec des Clectrodes de PdO ou de Au nues ne prCsentent pratiquement pas de changements. Les modkles qui ne considkrent que les propriCtCs globales des solutions n'arrivent pas h expliquer les changements observCs dans les frkquences. Par ailleurs, un modkle par circuit Cquivalent incorporant les propriCtCs rhCologiques des couches d'acide nuclCique sur la surface du senseur permet d'expliquer les diverses rkponses.

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Section: Conductivity (D'nil)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rheological and interfacial properties of nucleic acid films studied by thickness – shear mode sensor and network analysis

Su¹,

Thompson²

1996

Can. J. Chem.

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“…f HG1/2 and f LG1/2 are the higher and lower frequencies at half-peak height in the G spectrum, and f B min and f B max are the frequencies at minimum and maximum B values, respectively, and all symbols are in their international units. In addition, Martin et al (2) have presented a series of equations of equivalent circuit parameters reflecting effects of the solution density (ρ L ) and viscosity (η L ), from which the relationship between R 1 and f 0 due to net changes in solution density and viscosity has been obtained as follows and validated experimentally (10)(11)(12),…”

Section: Introductionmentioning

confidence: 99%

In Situ Monitoring of Generation and Precipitation of Ferric Hydroxide Sol with a Piezoelectric Quartz Crystal Impedance Analyzer

Zhang

Xie

Yin

et al. 2001

Journal of Colloid and Interface Science

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“…Second, the swelling of polymer films in aqueous solution may cause the lose of part of the sensitivity and stability of the piezoelectric immunosensor. A silane layer is another useful coating, 7,8 however, because it can be integrated with the quartz crystal, the quartz-crystal resonator can not be used repeatedly.…”

mentioning

confidence: 99%

Quartz-Crystal Microbalance Immunosensor for Schistsoma-Japonicum-Infected Rabbit Serum

Shen

Wang

et al. 2003

ANAL. SCI.

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A quartz-crystal microbalance immunosensor (QCM) has been developed for the direct determination of Schistosomajaponicum-infected rabbit serum. A self-assembled monolayer with carboxyl groups was first coated on a gold electrode of a quartz-crystal resonator by the spontaneous adsorption of 3-mercaptopropionic acid. Schistosoma-japonicum molecular antigen of 32 kD molecular weight was then covalently attached to the crystal surface. The QCM immunosensor was used to detect infected rabbit serum (IRS49-2000); a maximum titer of 1:800 was achieved.

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Adsorption, immobilization, and hybridization of DNA studied by the use of quartz crystal oscillators

Abstract: Quartz crystal oscillators have been used as microbalances, the so-called quartz crystal microbalance, because their resonant frequencies change by mass loading onto the surface

Cited by 140 publications

References 5 publications

Rheological and interfacial properties of nucleic acid films studied by thickness – shear mode sensor and network analysis

Rheological and interfacial properties of nucleic acid films studied by thickness – shear mode sensor and network analysis

In Situ Monitoring of Generation and Precipitation of Ferric Hydroxide Sol with a Piezoelectric Quartz Crystal Impedance Analyzer

Quartz-Crystal Microbalance Immunosensor for Schistsoma-Japonicum-Infected Rabbit Serum

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