Adsorption from solution: An experiment to illustrate the Langmuir adsorption isotherm

Duff, David G.; Ross, Sheina M. C.; Vaughan, D. H.

doi:10.1021/ed065p815

Cited by 48 publications

(34 citation statements)

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“…K ads is the ratio of rate constant for adsorption to that of desorption. 49 For systems with multiple binding sites, a K -value can be estimated using the Langmuir–Freundlich equation. 50,51 …”

Section: Methodsmentioning

confidence: 99%

Antibody-like Biorecognition Sites for Proteins from Surface Imprinting on Nanoparticles

Bhakta

Seraji

Suib

et al. 2015

ACS Appl. Mater. Interfaces

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Natural antibodies are used widely for important applications such as biomedical analysis, cancer therapy, and directed drug delivery, but they are expensive and may have limited stability. This study describes synthesis of antibody-like binding sites by molecular imprinting on silica nanoparticles (SiNP) using a combination of four organosilane monomers with amino acid-like side chains providing hydrophobic, hydrophilic, and H-bonding interactions with target proteins. This approach provided artificial antibody (AA) nanoparticles with good selectivity and specificity to binding domains on target proteins in a relatively low-cost synthesis. The AAs were made by polymer grafting onto SiNPs for human serum albumin (HSA) and glucose oxidase (GOx). Binding affinity, selectivity, and specificity was compared to several other proteins using adsorption isotherms and surface plasmon resonance (SPR). The Langmuir–Freundlich adsorption model was used to obtain apparent binding constants (KLF) from binding isotherms of HSA (6.7 × 104) and GOx (4.7 × 104) to their respective AAs. These values were 4–300 fold larger compared to a series of nontemplate proteins. SPR binding studies of AAs with proteins attached to a gold surface confirmed good specificity and revealed faster binding for the target proteins compared to nontarget proteins. Target proteins retained their secondary structures upon binding. Binding capacity of AAHSA for HSA was 5.9 mg HSA/g compared to 1.4 mg/g for previously report imprinted silica beads imprinted with poly(aminophenyl)boronic acid. Also, 90% recovery for HSA spiked into 2% calf serum was found for AAHSA.

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Section: Methodsmentioning

confidence: 99%

Antibody-like Biorecognition Sites for Proteins from Surface Imprinting on Nanoparticles

Bhakta

Seraji

Suib

et al. 2015

ACS Appl. Mater. Interfaces

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“…The Langmuir adsorption isotherm model is based on the following assumptions, although some exceptions have been reported [43]:…”

Section: Flocculation Isothermsmentioning

confidence: 99%

Removal of anionic dyes in aqueous solution by flocculation with cellulose ampholytes

Kono

Kusumoto

2015

Journal of Water Process Engineering

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a b s t r a c tCellulose ampholytes (CAms) prepared from biocompatible and biodegradable carboxymethyl cellulose (CMC) could represent an environmentally friendly alternative for the efficient removal of anionic dyes from water effluents. In order to evaluate their potential for this application, a series of CAms with a degree of cationic substitution (DS C ) between 0.26 and 1.08 were prepared from sodium CMC, by cationization with 2,3-epoxypropyltrimethylammonium chloride. The flocculation ability of the CAms against the anionic dye Acid Red 13 (AR13) was found to strongly depend on the DS C as well as on the pH of the solution. The highest flocculation ability was observed at lower pH and higher DS C : a color removal as high as 99% was achieved at pH 3 using a CAm with the highest DS C . The kinetic analysis revealed that the flocculation of AR13 can be accurately fitted by a pseudo-second order kinetic model, which thus allows to predict the flocculation behavior of CAms under these conditions. Besides AR13, the ampholytes also showed high flocculation ability against Acid Red 9 and Acid Blue 13. The flocculation behavior of these anionic dyes followed the Langmuir adsorption isotherm model, which revealed the maximum flocculation capacity of a CAm for these dyes. Moreover, the analysis of the flocculation isotherm data and the SEM observation of the formed flocs allowed to determine the flocculation mechanism.

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“…Although these results have been confirmed by many experiments [5,16], the fMIM model cannot describe the experimental results [18] obtained for high solute concentrations. In particular, some experimental data support the view that the Langmuir isotherm [11,12,19] is best suited for describing the dynamic equilibrium between the mobile and immobile phases (when C and Q do not depend on time). Due to this fact a nonlinear kinetic equation is introduced in the MIM approach.…”

Section: Fractional Mimmentioning

confidence: 79%

“…(2.2)) with a = ωQ 0 . In the stationary case when ∂ t Q = 0, we obtain the Langmuir-type adsorption isotherm [18,19]…”

Section: Nonlinear Mim Modelmentioning

confidence: 99%

A Non-Linear Model for Solute Transport, Accounting for Sub-diffusive Concentration Decline and Sorption Saturation

Maryshev

2016

Math. Model. Nat. Phenom.

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Solute transport in porous media is very often complicated by solute immobilization on a solid matrix of porous media. Usually, immobilization is accounted by the mobile/immobile media approach (MIM). However, solute immobilization is very complicated phenomena with a variety of specific features. Therefore, in the literature there have been a lot of specific MIM-type models. Usually each model is constructed for to account one specific feature. Examples are the power decline of concentration in the large time limit at small concentration and the limitation of the immobilization process at high concentrations. Both effects have been evidenced by experiments. The present paper develops a hybrid nonlinear fractional MIM model potentially able to describe the above two features. A step-by-step process of constructing the nonlinear fractional MIM model is presented, and the main properties of the new model are discussed. Two limiting cases describe power law decline and sorption saturation have predicted by the new model equations. Numerical simulations illustrate limiting cases and the capabilities of new nonlinear fractional model.

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Adsorption from solution: An experiment to illustrate the Langmuir adsorption isotherm

Cited by 48 publications

References 0 publications

Antibody-like Biorecognition Sites for Proteins from Surface Imprinting on Nanoparticles

Antibody-like Biorecognition Sites for Proteins from Surface Imprinting on Nanoparticles

Removal of anionic dyes in aqueous solution by flocculation with cellulose ampholytes

A Non-Linear Model for Solute Transport, Accounting for Sub-diffusive Concentration Decline and Sorption Saturation

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