Adsorption Equilibrium and Thermodynamics of Tea Theasinensins on HP20—A High-Efficiency Macroporous Adsorption Resin

Xiong, Chunhua; Cui, Hongchun; Xue, Jinjin; Wang, Wei; Wang, Weiwei; Le, Ting; Chen, Lin; Engelhardt, Ulrich H.; Jiang, Heyuan

doi:10.3390/foods10122971

Cited by 6 publications

(2 citation statements)

References 32 publications

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“…Furthermore, the value of 1/ n , which was utilized to assess the feasibility of the adsorption behavior, was determined to be significantly less than 0.5. Therefore, we can conclude that the adsorption process of DNA on MM@PGMA–PA-Ti 4+ microspheres was quite easy and fast. − In all, MM@PGMAPA-Ti 4+ exhibited a substantial adsorption capacity for DNA.…”

Section: Resultsmentioning

confidence: 83%

Immobilization of Multivalent Titanium Cations on Magnetic Composite Microspheres for Highly Efficient DNA Extraction and Amplification

Wang,

Fei,

Zhou

et al. 2023

ACS Appl. Mater. Interfaces

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Magnetic-assisted DNA testing technology has attracted much attention in genetics, clinical diagnostics, environmental microbiology, and molecular biology. However, achieving satisfying DNA adsorption and desorption efficiency in real samples is still a big challenge. In this paper, a new kind of high-quality magnetic composite microsphere of MM@PGMA− PA-Ti 4+ was designed and prepared for DNA extraction and detection based on the strong interaction of Ti 4+ and phosphate groups. By taking the advantages of high magnetic susceptibility and high Ti 4+ content, the MM@ PGMA−PA-Ti 4+ microspheres possessed remarkable extraction capacity for mimic biological samples (salmon sperm specimens) with saturated loadings up to 533.0 mg/g. When the DNA feeding amount was 100 μg and the MM@ PGMA−PA-Ti 4+ dosage was 1 mg, the adsorption and desorption efficiencies were 80 and 90%, respectively. The kinetic and equilibrium extraction data were found to fit well with the pseudo-second-order model and Freundlich isotherm model. Furthermore, the MM@PGMA−PA-Ti 4+ microspheres were successfully employed for DNA extraction from mouse epithelial-like fibroblasts. The extraction ability (84 ± 4 μg/mg) and DNA purity were superior to the comparative commercial spin kits, as evaluated by electrophoresis assays and qPCR analysis. The experimental results suggest that the MM@PGMA−PA-Ti 4+ microspheres possess great potential as an adsorbent for DNA purification from complex biological samples.

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Section: Resultsmentioning

confidence: 83%

Immobilization of Multivalent Titanium Cations on Magnetic Composite Microspheres for Highly Efficient DNA Extraction and Amplification

Wang,

Fei,

Zhou

et al. 2023

ACS Appl. Mater. Interfaces

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“…Catechins, featuring secondary metabolites in tea, are vital contributors to tea infusions’ bitter and astringent taste. However, they are chemically active, and part of them may undergo oxidation and polymerization to form new flavor molecules like theaflavins (TFs) and theasinensins during oolong tea processing ( 4 ). These intermediates can continue to form complex molecules under certain conditions ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of the Baking Process on the Chemical Composition, Sensory Quality, and Bioactivity of Tieguanyin Oolong Tea

et al. 2022

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Tieguanyin oolong tea (TOT), a semi-oxidized tea originating from Anxi county in China, is categorized into jade TOT, medium-baked TOT, and deep-baked TOT, based on different baking processes. To study the effects of baking, chemical analysis, sensory evaluation, and bioactivity assessments of the three TOTs were conducted. The results indicated that the baking process promoted the formation of colored macromolecules (e.g., theabrownins), which affected the color of tea infusion. Free amino acids underwent the Maillard reaction and generated specific Maillard reaction products, such as 5-hydroxymethylfurfural and furfural, which modified the taste and aroma. Floral and fresh volatiles were remarkably reduced, while multiple new volatiles were produced, forming a typically baked aroma. The antioxidant activity and antibacterial activity were reduced after baking, which might be associated with the decrease of monomeric catechins. These results provide a scientific basis for understanding the changes caused by the baking process.

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Enantioselective resolution of (R,S)-DMPM to prepare (R)-DMPM by an adsorbed-covalent crosslinked esterase PAE07 from Pseudochrobactrum asaccharolyticum WZZ003

Zhang

Wei

Zhou

et al. 2022

Bioprocess Biosyst Eng

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Adsorption Equilibrium and Thermodynamics of Tea Theasinensins on HP20—A High-Efficiency Macroporous Adsorption Resin

Cited by 6 publications

References 32 publications

Immobilization of Multivalent Titanium Cations on Magnetic Composite Microspheres for Highly Efficient DNA Extraction and Amplification

Immobilization of Multivalent Titanium Cations on Magnetic Composite Microspheres for Highly Efficient DNA Extraction and Amplification

Effects of the Baking Process on the Chemical Composition, Sensory Quality, and Bioactivity of Tieguanyin Oolong Tea

Enantioselective resolution of (R,S)-DMPM to prepare (R)-DMPM by an adsorbed-covalent crosslinked esterase PAE07 from Pseudochrobactrum asaccharolyticum WZZ003

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