Adsorption and flocculation by polymers and polymer mixtures

Gregory, John; Bárány, Sándor

doi:10.1016/j.cis.2011.06.004

Cited by 372 publications

(228 citation statements)

References 74 publications

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“…4,[7][8][9][10][11][12] Also, product recovery for a harvest method utilizing flocculation will be dependent on the volume of cell culture fluid (CCF) recovered and any potential interaction of the product with the flocculent (flocculated cell/cellular debris particle) or the polycationic polymer. The parameters investigated in the development of a CHO cell culture PDADMAC flocculation harvest method are discussed in the following sections.…”

Section: Resultsmentioning

confidence: 99%

“…4,[7][8][9][10][11][12] Since PDADMAC binds to the negatively-charged cells (both viable and non-viable cells) and cellular debris in the cell culture broth, a high cell density cell culture process requires a larger flocculation dose per volume compared to a lower cell density culture process to achieve the optimum flocculation performance (clarity, product recovery, and settling period). To account for cell culture process variability from process to process and run-to-run, a dosing regimen based on the cellular mass is desired.…”

Section: Pdadmac Dosingmentioning

confidence: 99%

“…Polycationic polymers bind the negatively-charged cells and cellular debris leading to the formation of larger particles that easily settled out or removed by centrifugation. A number of polycationic polymers have been used to flocculate cells, including polyethyleneimine, poly (diallyldimethylammonium chloride) or PDADMAC, chitosan, polycationic polyacrylamides, and partially benzylated poly(allylamine), e.g., smart Polymer E. [7][8][9][10][11][12][13] PDADMAC has several advantages over the other polycationic polymers that have been used to clarify cell broth. In contrast to some of the other polycationic polymers, PDADMAC is supplied as a chloride salt solution and requires no sample conditioning prior to use.…”

Section: Introductionmentioning

confidence: 99%

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PDADMAC flocculation of Chinese hamster ovary cells: Enabling a centrifuge-less harvest process for monoclonal antibodies

McNerney

Thomas

Senczuk

et al. 2015

mAbs

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(2015) PDADMAC flocculation of Chinese hamster ovary cells: Enabling a centrifuge-less harvest process for monoclonal antibodies, mAbs, 7:2, 413-427, DOI: 10.1080DOI: 10. /19420862.2015 To link to this article: https://doi.org/10. 1080/19420862.2015 High titer (>10 g/L) monoclonal antibody (mAb) cell culture processes are typically achieved by maintaining high viable cell densities over longer culture durations. A corresponding increase in the solids and sub-micron cellular debris particle levels are also observed. This higher burden of solids ( 15%) and sub-micron particles typically exceeds the capabilities of a continuous centrifuge to effectively remove the solids without a substantial loss of product and/or the capacity of the harvest filtration train (depth filter followed by membrane filter) used to clarify the centrate. We discuss here the use of a novel and simple two-polymer flocculation method used to harvest mAb from high cell mass cell culture processes. The addition of the polycationic polymer, poly diallyldimethylammonium chloride (PDADMAC) to the cell culture broth flocculates negatively-charged cells and cellular debris via an ionic interaction mechanism. Incorporation of a non-ionic polymer such as polyethylene glycol (PEG) into the PDADMAC flocculation results in larger flocculated particles with faster settling rate compared to PDADMAC-only flocculation. PDADMAC also flocculates the negatively-charged sub-micron particles to produce a feed stream with a significantly higher harvest filter train throughput compared to a typical centrifuged harvest feed stream. Cell culture process variability such as lactate production, cellular debris and cellular densities were investigated to determine the effect on flocculation. Since PDADMAC is cytotoxic, purification process clearance and toxicity assessment were performed.

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Section: Resultsmentioning

confidence: 99%

Section: Pdadmac Dosingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PDADMAC flocculation of Chinese hamster ovary cells: Enabling a centrifuge-less harvest process for monoclonal antibodies

McNerney

Thomas

Senczuk

et al. 2015

mAbs

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“…It is common knowledge that addition of oppositely charged coagulants and flocculants to a colloidal dispersion usually leads to a significant decrease in the zeta potential and to a change in the sign of the particle charge, which is accompanied by destabilization of the system. As a result, beneficial information on the colloid destabilization mechanisms is achieved thanks to the data on zeta potential [9]. Toward this aim, in this study, the effects of multivalent ions and polymers on the settling velocity, supernatant turbidity, zeta potential and suspension pH were investigated for kaolin suspensions.…”

Section: Introductionmentioning

confidence: 99%

“…To achieve charges at varying densities, the level of hydrolysis may be regulated. For flocculation, the molecular weight and charge density of polymers can be defined as the most significant characteristics [9]. Flocculation may involve different destabilization mechanisms including polymer bridging, charge neutralization, formation of polymer-particle surface complex or combination of these mechanisms, based on the characteristics of polymers and structure of tailings [10,11].…”

Section: Introductionmentioning

confidence: 99%

Kaolen Süspansi̇yonlarinin Susuzlaştirilmasinda Koagülant Ve Flokülantlarin Etki̇si̇

Önen

Göçer²,

Taner³

2018

Ömer Halisdemir Üniversitesi Mühendislik Bilimleri Dergisi

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Kaolin tailings are commonly generated in the mineral industry. They are invariably negatively charged and consequently tend to form stable dispersions with poor flocculation characteristics. Coagulation-flocculation treatments are suitable methods for removing colloidal particles from wastewater. In this study, the effects of mono/multivalent ions (coagulants) and polymers (flocculants) on the sedimentation and electrokinetic behaviours of kaolin have been investigated. In experimental studies, Al 2 (SO 4 ) 3 , FeCl 3 , MgCl 2 , CaCl 2 , NaCl were used as coagulant, while as flocculant anionic (A150), cationic (C521) and nonionic (N100) polymers were used. Isoelectric point of kaolin was determined as pH 4.2. The effectiveness of coagulants increased with the increase in the ionicity degree of the metal ions. Among the coagulants, FeCl 3 provided the highest efficiency (91%). Low sedimentation velocities (6.3-12.2 mm/min) were obtained with coagulants. The highest sedimentation efficiency (94%) was achieved with anionic flocculants and the same sedimentation velocity (58 mm/min) was reached with all flocculants.Keywords: Kaolin, dewatering, polymer, metal salts, zeta potential KAOLEN SÜSPANSİYONLARININ SUSUZLAŞTIRILMASINDA KOAGÜLANT VE FLOKÜLANTLARIN ETKİSİ ÖZKaolin atıkları mineral endüstrisinde yaygın olarak üretilir. Negatif yüklü olan bu atıklar zayıf flokülasyon özelliklerine sahip kararlı dağılımlar oluşturmaya eğilimlidirler. Koagülasyon-flokülasyon işlemleri, koloidal parçacıkları atık sulardan uzaklaştırmak için uygun yöntemlerdir. Bu çalışmada, mono/multivalent iyonların (koagülant) ve polimerlerin (flokülant) kaolenin çökelme ve elektrokinetik davranışlarına etkisi araştırılmıştır. Deneysel çalışmalarda, koagülant olarak Al 2 (SO 4 ) 3 , FeCl 3 , MgCl 2 , CaCl 2 , NaCl ve flokülant olarak, anyonik (A150), katyonik (C521) ve noniyonik (N100) polimerler kullanılmıştır. Kaolenin sıfır yük noktası pH 4.2 olarak belirlenmiştir. Koagülantların etkinliği, metal iyonlarının iyoniklik derecesinin artması ile artmıştır. Koagülantlar arasında FeCl 3 en yüksek verimi (%91) sağlamıştır. Koagülantlar ile düşük sedimantasyon hızları (6.3-12.2 mm/dk) elde edilmiştir. En yüksek sedimantasyon verimliliği (%94) anyonik flokülant ile sağlanmış ve tüm flokülantlar ile yaklaşık olarak aynı çökelme hızına (58 mm/dk) ulaşılmıştır.

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Sodalite Solids Formation at the Surface of Iron Oxide and Its Impact on Flocculation

Senaputra¹,

Fawell²,

Jones³

et al. 2013

Light Metals 2013

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Adsorption and flocculation by polymers and polymer mixtures

Cited by 372 publications

References 74 publications

PDADMAC flocculation of Chinese hamster ovary cells: Enabling a centrifuge-less harvest process for monoclonal antibodies

PDADMAC flocculation of Chinese hamster ovary cells: Enabling a centrifuge-less harvest process for monoclonal antibodies

Kaolen Süspansi̇yonlarinin Susuzlaştirilmasinda Koagülant Ve Flokülantlarin Etki̇si̇

Sodalite Solids Formation at the Surface of Iron Oxide and Its Impact on Flocculation

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