Adsorption and Desorption Behaviors of Bovine Serum Albumin on Gelatin/Chitosan Sponge

Furuike, Tetsuya; Chaochai, Thitirat; Komoto, Daiki; Tamura, Hiroshi

doi:10.4236/msce.2017.51015

Cited by 5 publications

(9 citation statements)

References 21 publications

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“…45 Protein charge is usually influenced by a function of pH. 48,49 Low pH proteins are positively charged, and high pH proteins are negatively charged. 49 However, in this study, BSA proteins' pH value will be fixed in the same range as the human synovial fluid; pH 7-7.6.…”

Section: Surface Charge Measurementmentioning

confidence: 99%

Effects of surface wettability and thermal conductivity on the wear performance of ultrahigh molecular weight polyethylene/graphite and ultrahigh molecular weight polyethylene/graphene oxide composites

Shahemi

Liza

Sawae

et al. 2022

Polymers for Advanced Techs

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Recent studies have found a rapid increase of ultrahigh molecular weight polyethylene (UHMWPE) wear in the presence of proteins from the synovial fluid. Due to UHMWPE's high hydrophobicity, it tends to adsorb a tremendous amount of proteins. Moreover, since UHMWPE has low thermal conductivity, a temperature rise in the center of the contact area due to frictional heating could cause protein denaturation from the synovial fluid. It has been shown that the denatured protein may increase the adhesive wear response. This study aimed to address the effects of graphite and graphene oxide (GO) addition on the wear properties of UHMWPE in protein environments. The surface properties were characterized using surface roughness profiler, surface energy evaluation, zeta potential, and Fourier transform infra-red (FTIR). Following that, wear properties of UHMWPE composite were evaluated using a multidirectional pin-on-disc wear test under a bovine serum lubricated condition. The worn surface of the UHMWPE composite sample was evaluated, and the dominating factors of wear properties were determined. The effect of protein adsorption on the composite surface was also assessed after the wear test. The hydrophilicity of UHWMPE/1.0GO is considered to be the dominant contribution determining protein adsorption in static conditions. UHMWPE composite's wear resistance improvement was primarily dominated by GO filler (1.0 wt%) near the sliding surface, which has improved the subsurface strength of the material and heat dissipation effect, which reduces the denaturation of the proteins.

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Section: Surface Charge Measurementmentioning

confidence: 99%

Effects of surface wettability and thermal conductivity on the wear performance of ultrahigh molecular weight polyethylene/graphite and ultrahigh molecular weight polyethylene/graphene oxide composites

Shahemi

Liza

Sawae

et al. 2022

Polymers for Advanced Techs

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“…Protein adsorption onto gelatin hydrogels cross-linked by CME-PRXs was evaluated according to previous reports [30]. Briefly, gelatin hydrogels cross-linked by CME-PRXs (width: 10 mm; length: 10 mm; height: 0.5 mm) were put onto 12-well plates.…”

Section: Protein Adsorption Assaymentioning

confidence: 99%

Cellular Orientation on Repeatedly Stretching Gelatin Hydrogels with Supramolecular Cross-Linkers

et al. 2019

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The cytocompatibility of biological and synthetic materials is an important issue for biomaterials. Gelatin hydrogels are used as biomaterials because of their biodegradability. We have previously reported that the mechanical properties of gelatin hydrogels are improved by cross-linking with polyrotaxanes, a supramolecular compound composed of many cyclic molecules threaded with a linear polymer. In this study, the ability of gelatin hydrogels cross-linked by polyrotaxanes (polyrotaxane–gelatin hydrogels) for cell cultivation was investigated. Because the amount of polyrotaxanes used for gelatin fabrication is very small, the chemical composition was barely altered. The structure and wettability of these hydrogels are also the same as those of conventional hydrogels. Fibroblasts adhered on polyrotaxane–gelatin hydrogels and conventional hydrogels without any reduction or apoptosis of adherent cells. From these results, the polyrotaxane–gelatin hydrogels have the potential to improve the mechanical properties of gelatin without affecting cytocompatibility. Interestingly, when cells were cultured on polyrotaxane–gelatin hydrogels after repeated stress deformation, the cells were spontaneously oriented to the stretching direction. This cellular response was not observed on conventional hydrogels. These results suggest that the use of a polyrotaxane cross-linking agent can not only improve the strength of hydrogels but can also contribute to controlling reorientation of the gelatin.

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“…Protein charge is usually found to be influenced by a function of pH [34][35]. Low pH proteins are positively charged and high pH proteins are negatively charged [35]. However, in this study, the pH value of BSA proteins used will be fixed as the same range as the human synovial fluid; pH 7-7.6 [21].…”

Section: Surface Chargementioning

confidence: 99%

“…Protein charge is usually found to be influenced by a function of pH [34][35]. Low pH proteins are positively charged and high pH proteins are negatively charged [35].…”

Section: Surface Chargementioning

confidence: 99%

Effect of Protein Interactions on the UHMWPE Composites After the Multidirectional Wear Test

Shahemi

Liza

Sawae

et al. 2021

Preprint

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Recent studies have found a rapid increase of ultrahigh molecular weight polyethylene (UHMWPE) wear in the presence of proteins from the synovial fluid. This is due to the high hydrophobicity surface of UHMWPE which allows the proteins to get interacted and adsorbed to the UHMWPE surface. Moreover, since UHMWPE has low thermal conductivity, the heat elevated produced from the articulating contact surfaces would be slowly dissipated and protein structure becoming denatured. The denatured proteins were claimed could increase the adhesive wear response. Thus, the main objective of this study is to evaluate the influence of the graphene oxide (GO) and graphite flake (GF) on the UHMWPE’s heat dissipation and wettability characteristics in regards to reducing the protein denaturation effect on the surface. The surface properties were characterized by using surface energy evaluation, zeta potential, and Fourier Transform Infra-red (FTIR). Following that, wear properties of each UHMWPE composite were evaluated by using a multidirectional pin-on-disc wear test under a lubricated condition. The worn surface of each pin sample was evaluated, and the dominating factors of wear properties were determined. In addition to that, the effect of protein absorption on the UHMWPE composites was also evaluated in this study. The results show both fillers (GO and GF) had no effect in altering the UHMWPE composites’ physicochemical properties. The improvement of UHMWPE composite’s wear properties was discovered to be primarily dominated by the existence of GO filler (1.0 wt%) near the sliding surface has improved the subsurface strength of the material and heat dissipation effect which reduces the denaturation of the proteins.

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Adsorption and Desorption Behaviors of Bovine Serum Albumin on Gelatin/Chitosan Sponge

Cited by 5 publications

References 21 publications

Effects of surface wettability and thermal conductivity on the wear performance of ultrahigh molecular weight polyethylene/graphite and ultrahigh molecular weight polyethylene/graphene oxide composites

Effects of surface wettability and thermal conductivity on the wear performance of ultrahigh molecular weight polyethylene/graphite and ultrahigh molecular weight polyethylene/graphene oxide composites

Cellular Orientation on Repeatedly Stretching Gelatin Hydrogels with Supramolecular Cross-Linkers

Effect of Protein Interactions on the UHMWPE Composites After the Multidirectional Wear Test

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