Adsorção de fibronectina a arcabouços de polihidroxibutirato aplicáveis à engenharia óssea

Mendonça, Roberta Helena; Thiré, Rossana Mara da Silva Moreira; Costa, Márcio Henrique Sá Netto; Filho, Fernando C. Silva

doi:10.1590/s0104-14282009000200012

Cited by 6 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PHAs have been usually pretreated or functionalized because of the hydrophobic and nonactive nature, followed by coating procedure to increase the efficiency of bio‐macromolecules introduction. Ethylenediamine‐treated PHB scaffolds led to an increasing adsorption of fibronectin that might assume a folded conformation to hide some Arg‐Gly‐Asp (RGD) domains . Porous PHBV matrix (ozone‐treatment and collagen coat) maintained the osteoblastic phenotype characteristics of the UMR‐106 cells .…”

Section: Preparation Of Cell Compatible Surfacementioning

confidence: 99%

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

Liu

Zhang

et al. 2017

Macro Materials & Eng

View full text Add to dashboard Cite

Biomaterials for in vivo application should induce positive interaction with various histocytes and inhibit bacteria inflection as well. Cells and/or bacteria response to the extracellular environment is therefore the basic principle to design the biomaterials surface in order to induce the specific biomaterial–biological interaction. Polyhydroxyalkanoate (PHAs) are of growing interests because of their natural origin, biodegradability, biocompatibility, and thermoplasticity; however, quite inert and intrinsic hydrophobic characteristics have hindered their extensive usage in medical applications. Surface modification of PHAs tailors the chemistry, wettability, and topography without altering the bulk properties, and introduces specific proteins/peptides and/or antibacterial agents to mediate cell–matrix interactions. This review describes the recent developments on the surface modification of PHAs to construct cell compatible and antibacterial surfaces.

show abstract

Section: Preparation Of Cell Compatible Surfacementioning

confidence: 99%

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

Liu

Zhang

et al. 2017

Macro Materials & Eng

View full text Add to dashboard Cite

show abstract

“…Polyhydroxybutyrate (PHB) have several uses in the medical and pharmaceutical industry [5], but the main application of this biopolymer is in the plastic industry due to the characteristics similar to those of polypropylene that allow the application of these polymer to produce of biodegradable plastics [6,7]. Root nodule bacteria are also able to produce PHB in root nodules, and investigations have shown the importance of these polymers on the biological nitrogen fixation [8].…”

Section: Introductionmentioning

confidence: 99%

Poly-β-hydroxybutyrate and Exopolysaccharide Biosynthesis by Bacterial Isolates from Pigeonpea [Cajanus cajan (L.) Millsp] Root Nodules

Fernandes‐Júnior

Oliveira

Rumjanek

et al. 2010

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The bacterial strains that are able to produce biopolymers that are applied in industrial sectors present a source of renewable resources. Some microorganisms are already applied at several industrial sectors, but the prospecting of new microbes must bring microorganisms that are feasible to produce interesting biopolymers more efficiently and in cheaper conditions. Among the biopolymers applied industrially, polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) stand out because of its applications, mainly in biodegradable plastic production and in food industry, respectively. In this context, the capacity of bacteria isolated from pigeonpea root nodules to produce EPS and PHB was evaluated, as well as the cultural characterization of these isolates. Among the 38 isolates evaluated, the majority presented fast growth and ability to acidify the culture media. Regarding the biopolymer production, five isolates produced more than 10 mg PHB per liter of culture medium. Six EPS producing bacteria achieved more than 200 mg EPS per liter of culture medium. Evaluating different carbon sources, the PHB productivity of the isolate 24.6b reached 69% of cell dry weight when cultured with starch as sole carbon source, and the isolate 8.1c synthesized 53% PHB in dry cell biomass and more than 1.3 g L⁻¹ of EPS when grown using xylose as sole carbon source.

show abstract

“…A seleção do material adequado para sua confecção é um critério importante, pois a natureza química do material tem influência sobre sua biocompatibilidade. As características ideais dos scaffolds são dependentes de sua aplicação, mas de maneira geral, devem ser biocompatíveis, biorreabsovíveis e com uma rede de poros de dimensões suficientes para permitir a penetração celular e melhorar a intercomunicação célula-matriz e a troca de nutrientes e produtos de degradação (Mendonça et al, 2009).…”

Section: Aplicação Na Engenharia De Tecidosunclassified

Modificação química do poli(3-hidroxibutirato) e preparação de membranas por eletrofiação para aplicação em biomateriais.

Sakaguti¹

View full text Add to dashboard Cite

Due to the increasing interest in biodegradable polymers, many studies have been conducted in order to obtain biodegradable polymers with improved mechanical and processing properties. The poly(3-hydroxybutyrate) (PHB) has been proposed as an ideal substitute for non-biodegradable polymers in commercial applications. However, its high crystallinity, thermal instability and high production costs are problems that have limited its commercialization. An alternative to modify it and improve its mechanical properties is through transesterification reactions with poly(εcaprolactone) (PCL), a synthetic polyester, also biodegradable and with high potential for use as a biomaterial. In this work, reactive extrusion of PHB/PCL blend was carried out, and the copolymer was characterized by solubility tests, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), hydrogen (1 H-NMR) and carbon (13 C-NMR) nuclear magnetic resonance spectroscopies. The results indicated that PHB was modified, resulting in the copolymer PHB-co-PCL, showing lower crystallinity than the homopolymers. Viscosity measurements of PHB-co-PCL solutions were performed and electrospinning tests were carried out under different conditions. The scanning electron microscopy analysis (SEM) indicated the formation of smooth and uniform fibers, with average diameter between 900-1200 nm. Cell viability analysis confirmed that the material is not cytotoxic, favoring its applicability for the production of porous fiber mats in tissue engineering.

show abstract

Adsorção de fibronectina a arcabouços de polihidroxibutirato aplicáveis à engenharia óssea

Cited by 6 publications

References 15 publications

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

Poly-β-hydroxybutyrate and Exopolysaccharide Biosynthesis by Bacterial Isolates from Pigeonpea [Cajanus cajan (L.) Millsp] Root Nodules

Modificação química do poli(3-hidroxibutirato) e preparação de membranas por eletrofiação para aplicação em biomateriais.

Contact Info

Product

Resources

About