Adrenomedullin promotes epithelial restitution of rat and human gastric mucosa in vitro

Fukuda, Kazuhito; Tsukada, Hideaki; Oya, Masanao; Onomura, M.; Kodama, Mitsuo; Nakamura, Hiroshi; Hosokawa, Munetaka; Seino, Yutaka

doi:10.1016/s0196-9781(98)00146-6

Cited by 27 publications

(19 citation statements)

References 20 publications

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“…Both peptides have been identified within the stomach. Adrenomedullin has been localized to gastric chief cells and CGRP-positive nerve fibers have been reported to exist not only around the blood vessels of the human stomach but also within the lamina propria mucosae (Fukuda et al 1999;Tani et al 1999). In vivo and in vitro studies indicate that the peptides are involved in the control of gastric acid production, whereas little is known of possible effects on enzyme secretion (Rossowski et al 1997).…”

Section: Gastrointestinal Tractmentioning

confidence: 99%

“…In vivo and in vitro studies indicate that the peptides are involved in the control of gastric acid production, whereas little is known of possible effects on enzyme secretion (Rossowski et al 1997). Another proposed function is protection and restitution of the gastric mucosal integrity in response to noxious stimuli (Fukuda et al 1999;Holzer 1998a;Szolcsanyi and Bartho 2001).…”

Section: Gastrointestinal Tractmentioning

confidence: 99%

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Calcitonin receptor-like receptor: identification and distribution in human peripheral tissues

Hagner

Stahl

Knoblauch

et al. 2002

Cell and Tissue Research

108

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We report here on the characterization and immunohistochemical localization in human tissues of calcitonin receptor-like receptor (CRLR) which was recently found to mediate the effects of both calcitonin gene-related peptide (CGRP) and adrenomedullin (ADM). Western blot analysis using antibodies raised against the first extracellular loop and the carboxy-terminal part of hCRLR, respectively, detected two major bands corresponding to about 70 and 60 kDa in membrane preparations of cultured endothelial cells and numerous organs including lung, heart ventricle and kidney. Immunohistochemical analysis of the cardiovascular system revealed CRLR-like immunoreactivity (CRLR-LI) in the endothelium of all blood vessels including large and small arteries, veins and capillaries, and in heart muscle cells and endocardium. The lung showed intense staining over the alveolar capillaries. Within the digestive tract, staining was observed over the cells lining the excretory ducts of the parotid gland, over the epithelium of the fundic glands of stomach, endocrine cells of the duodenum and ileum and some myenteric ganglia. The kidney presented staining of the juxtaglomerular arteries, the glomerular capillaries and chief cells of the collecting duct. Within the endocrine organs, a strong CRLR-LI signal was observed over the Langerhans islets, and weak immunoreactivity in the Leydig cells of testis. Spleen showed intense staining in trabecular veins and sinuses. Macrophages displayed a variable immunoreactivity. Our data demonstrate a wide distribution of CRLR throughout the human body and suggest CRLR to be involved in the mediation of a variety of actions in addition to vascular control.

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Section: Gastrointestinal Tractmentioning

confidence: 99%

Section: Gastrointestinal Tractmentioning

confidence: 99%

Calcitonin receptor-like receptor: identification and distribution in human peripheral tissues

Hagner

Stahl

Knoblauch

et al. 2002

Cell and Tissue Research

108

View full text Add to dashboard Cite

show abstract

“…Both peptide immunoreactivities can be detected in neuroendocrine cells, being specially abundant in the enterochromaffin-like and chief cells of the gastric fundus [124,125] as well as in the base of the glandular epithelia in the pyloric mucosa [126]. Adrenomedullin is also found in the gastrin-containing cells in the pyloric glands [127], and AM mRNA signals are present in nonendocrine cells in the basal half of the mucosa [123].…”

Section: Adrenomedullin Distribution In the Gastrointestinal Tractmentioning

confidence: 99%

“…Apart from its protective action, AM promotes and accelerates epithelial restitution of previously damaged rat and human mucosa [125]. The rapid reestablishment of epithelial integrity is an important endogenous protective mechanism against superficial injury in the stomach.…”

Section: Gastroprotective Effect Of Ammentioning

confidence: 99%

“…The rapid reestablishment of epithelial integrity is an important endogenous protective mechanism against superficial injury in the stomach. In vitro studies demonstrate that AM enhances recovery of mucosal integrity after mild but not severe damage of gastric mucosa caused by the exposure to a hyperosmolar NaCl solution [125]. Because AM is synthesized in chief cells of the gastric mucosa, it is probable that it would be secreted in large quantities to accelerate restitution after mucosal damage.…”

Section: Gastroprotective Effect Of Ammentioning

confidence: 99%

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