Adrenomedullin inhibits spontaneous and bradykinin‐induced but not oxytocin‐ or prostaglandin F<sub>2α</sub>‐induced periodic contraction of rat uterus

Yanagita, Toshihiko; Yamamoto, Ryōichi; Sugano, Tsukasa; Kobayashi, Hideyuki; Uezono, Yasuhito; Yokoo, Hiroki; Shiraishi, Seiji; Minami, Shin-ichi; Wada, Akihiko

doi:10.1038/sj.bjp.0703500

Cited by 40 publications

(24 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With regard to the reproductive system, ADM has been reported to generally act as a relaxant or vasodilator (Makino et al 1999, Yanagita et al 2000. However, the data presented in this paper show that locally expressed ADM can also function in the uterus in a more transient manner, since estrogen-induced ADM gene expression in the uterus only occurs in the stroma over a short period.…”

Section: Discussioncontrasting

confidence: 45%

The estrogen-responsive adrenomedullin and receptor-modifying protein 3 gene identified by DNA microarray analysis are directly regulated by estrogen receptor

Watanabe¹,

Takahashi²,

Kobayashi³

et al. 2006

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Recent studies have revealed that hundreds of genes in the uterus are activated by estrogen. Their expression profiles differ over time and doses and it is not clear whether all these genes are directly regulated by estrogen via the estrogen receptor. To select the genes that may be regulated by estrogen, we treated mice with several doses of estrogen and searched for those genes whose dose-response expression pattern mirrored the uterine growth pattern. Among those genes, we found that the dose-dependent expression of the adrenomedullin (ADM) gene correlated well with the uterotrophic effect of estrogen. ADM expression is induced early after estrogen administration and is restricted to the endometrial stroma. The spatiotemporal gene expression pattern of ADM was similar to that of receptor-modifying protein 3 (RAMP3). RAMP3 is known to modify calcitonin gene-related receptor (CRLR) so that it can then serve as an ADM receptor. Chromatin immunoprecipitation assays indicated that the estrogen receptor binds directly to the ADM promoter region and RAMP3 intron after estrogen administration. It was also shown that neither the ADM nor RAMP3 gene could be activated in estrogen receptor--null mouse. Although uterine ADM expression has been reported to occur in the myometrium, our observations indicate that estrogen-induced ADM is also expressed in the uterine stroma and that such variable, spatiotemporally regulated ADM expression contributes to a wider range of biological effects than previously expected.

show abstract

Section: Discussioncontrasting

confidence: 45%

The estrogen-responsive adrenomedullin and receptor-modifying protein 3 gene identified by DNA microarray analysis are directly regulated by estrogen receptor

Watanabe¹,

Takahashi²,

Kobayashi³

et al. 2006

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…Studies in the rat have shown that AM inhibits uterine smooth muscle (spontaneous and bradykinin-induced) contraction [95] and that this effect may be mediated through an increase in cAMP [96]. However, Al-Ghafra et al [97] found significant increases in AM concentrations in the amnion and choriodecidua from women in labor compared with those from women not in labor, for both term and pre-term gestations.…”

Section: Preterm Deliverymentioning

confidence: 95%

Adrenomedullin: Multiple functions in human pregnancy

et al. 2004

View full text Add to dashboard Cite

Adrenomedullin is a 52 amino acid peptide originally isolated from human phaeochromocytoma in 1993. It was initially demonstrated to have profound effects on the vasculature including vasodilatation and subsequently promotion of angiogenesis. Since then it has become apparent that it has a wide range of other biological actions including regulation of cell growth and differentiation. Successful pregnancy outcome relies on establishing and maintaining throughout gestation an efficient blood supply to the fetus. This allows the exchange of nutrients, oxygenation of fetal blood and removal of cytotoxins from the fetus, such as carbon dioxide. One of the most important local adaptations to pregnancy is the change in maternal blood flow to the implantation site. Evidence now points towards a vital role for adrenomedullin in the regulation of placentation. It appears that adrenomedullin may play important roles in the regulation of fetal perfusion both in normal and in compromised pregnancies. However, most studies have focused on measuring adrenomedullin levels and studying its expression as well as that of its receptors. More functional studies are now required to elucidate the underlying mechanisms involved.

show abstract

“…This effect is prevented by antagonist of AM receptors or calcitonin gene-related peptide (CGRP) receptors. AM inhibits BK-induced periodic uterine contraction, but is unable to counteracts periodic contraction induced by OT or PGF2α, thus showing that AM is able to inhibit both spontaneous and BK-induced periodic contraction of uterus via activating receptors for AM and CGRP [24].…”

Section: Oxytocin (Ot)mentioning

confidence: 98%

Bradykinin Signal Pathways Modulate Neurohypophyseal Hormones Secretion

Iovino¹

2014

J Autacoids

View full text Add to dashboard Cite

Adrenomedullin inhibits spontaneous and bradykinin‐induced but not oxytocin‐ or prostaglandin F_2α‐induced periodic contraction of rat uterus

Cited by 40 publications

References 23 publications

The estrogen-responsive adrenomedullin and receptor-modifying protein 3 gene identified by DNA microarray analysis are directly regulated by estrogen receptor

The estrogen-responsive adrenomedullin and receptor-modifying protein 3 gene identified by DNA microarray analysis are directly regulated by estrogen receptor

Adrenomedullin: Multiple functions in human pregnancy

Bradykinin Signal Pathways Modulate Neurohypophyseal Hormones Secretion

Contact Info

Product

Resources

About

Adrenomedullin inhibits spontaneous and bradykinin‐induced but not oxytocin‐ or prostaglandin F2α‐induced periodic contraction of rat uterus

Cited by 40 publications

References 23 publications

The estrogen-responsive adrenomedullin and receptor-modifying protein 3 gene identified by DNA microarray analysis are directly regulated by estrogen receptor

The estrogen-responsive adrenomedullin and receptor-modifying protein 3 gene identified by DNA microarray analysis are directly regulated by estrogen receptor

Adrenomedullin: Multiple functions in human pregnancy

Bradykinin Signal Pathways Modulate Neurohypophyseal Hormones Secretion

Contact Info

Product

Resources

About

Adrenomedullin inhibits spontaneous and bradykinin‐induced but not oxytocin‐ or prostaglandin F_2α‐induced periodic contraction of rat uterus