Adrenocorticotropic hormone controls Concanavalin A activation of rat lymphocytes by modulating IL-2 production

Wermerskirchen, Angela S.; LaTocha, Dorian; Clarke, Benjamin L.

doi:10.1016/s0024-3205(00)00810-9

Cited by 24 publications

(19 citation statements)

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“…5). Meanwhile, ConA (5 lg ml -1 ), a well known T-cell mitogen inducing cytokine production in lymphocytes, induced the expression level of IL-2 to 1,032 pg ml -1 , in agreement with the range of 1,011-1,229 pg ml -1 reported previously (Wermerskirchen et al 2000).…”

Section: Induced Expression Of Recombinant Fip and Sds-page Analysissupporting

confidence: 90%

Molecular cloning and recombinant expression of a gene encoding a fungal immunomodulatory protein from Ganoderma lucidum in Pichia pastoris

Lin

Hao

et al. 2008

World J Microbiol Biotechnol

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The fungal immunomodulatory proteins (FIPs) are a new protein family identified from several edible and medical mushrooms and play an important role in antitumor, anti-allergy and immunomodulating activities. A gene encoding the FIP was cloned from the mycelia of Changbai Lingzhi (Ganoderma lucidum) and recombinant expressed in the Pichia pastoris expression system. SDS-PAGE, amino acid composition and circular dichroism analyses of the recombinant FIP (reFIP) indicated that the gene was correctly and successfully expressed. In vitro assays of biological activities revealed that the reFIP exhibited similar immunomodulating capacities as native FIPs. The reFIP significantly stimulated the proliferation of mouse spleen lymphocytes and apparently enhanced the expression level of interleukin-2 released from the mouse splenocytes. In addition, anti-tumor activity assay showed that the reFIP could inhibit the proliferation of human leukemia-NB4 by inducing the cell apoptosis to a degree of about 32.4%. Taken together, the FIP gene from Changbai G. lucidum has been integrated into the yeast genome and expressed effectively at a high level (about 191.2 mg l -1 ). The reFIP possessed very similar biological activities to native FIPs, suggesting its potential application as a food supplement or immunomodulating agent in pharmaceuticals and even medical studies.

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Section: Induced Expression Of Recombinant Fip and Sds-page Analysissupporting

confidence: 90%

Molecular cloning and recombinant expression of a gene encoding a fungal immunomodulatory protein from Ganoderma lucidum in Pichia pastoris

Lin

Hao

et al. 2008

World J Microbiol Biotechnol

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“…We know both ␣MSH and ACTH are equivalent competitors for ACTH binding while AGRP is a poor competitor (3). Thus, our findings indicate that MC5-R is found on the rat lymphocytes and could be responsible for mediating the ACTH modulating effects of activated lymphocytes (7).…”

Section: Figmentioning

confidence: 56%

Expression of the Melanocortin 5 Receptor on Rat Lymphocytes

Akbulut

Byersdorfer

Larsen

et al. 2001

Biochemical and Biophysical Research Communications

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“…In various immune cells, such as the rat thymus cells, spleen cells, T cells and B cells, immune ACTH and precursor are identified indicating that the HPA axis is closely associated with the cellular immune function. ACTH regulates the production of IL-2 and regulates the immune system by ACTH receptors expressed on immune cells (18)(19)(20). IL-2 is mainly produced by T cells or the T cell line, and it plays an important role in the immune adjustment.…”

Section: Discussionmentioning

confidence: 99%

Effect of acute heat stress on adrenocorticotropic hormone, cortisol, interleukin-2, interleukin-12 and apoptosis gene expression in rats

et al. 2015

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Abstract. The aim of the present study was to investigate the effect of acute heat stress on the neuroendocrine and immunological function in rats. Male Sprague-Dawley rats were randomly divided into two groups and respectively exposed to heat (32˚C) or to room temperature (24˚C). After 7 days of heat exposure, the heat-stress rat model was established. The organ coefficients of the pituitary and adrenal glands were determined. The body temperature was measured by telemetry. The average contents of adrenocorticotropic hormone (ACTH), cortisol (Cor), interleukin-2 (IL-2) and IL-12 in serum were detected. The expression of apoptotic genes in the spleen was measured. The results showed that acute heat stress did not evidently affect the body temperature and body weight (P>0.05), but the exposure increased the organ coefficients of the pituitary and adrenal glands (P<0.05). Heat exposure significantly elevated the level of ACTH, Cor, IL-2 and IL-12 (P<0.05). The expression of caspase-3 and Bax were not changed significantly (P>0.05), while Bcl2 was reduced (P<0.05).

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Adrenocorticotropic hormone controls Concanavalin A activation of rat lymphocytes by modulating IL-2 production

Cited by 24 publications

References 0 publications

Molecular cloning and recombinant expression of a gene encoding a fungal immunomodulatory protein from Ganoderma lucidum in Pichia pastoris

Molecular cloning and recombinant expression of a gene encoding a fungal immunomodulatory protein from Ganoderma lucidum in Pichia pastoris

Expression of the Melanocortin 5 Receptor on Rat Lymphocytes

Effect of acute heat stress on adrenocorticotropic hormone, cortisol, interleukin-2, interleukin-12 and apoptosis gene expression in rats

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