Adrenergic innervation of coronary arteries and ventricular myocardium in the pig: fluorescence microscopic appearance in the normal state and after ischemia

Holmgren, Susanne; Abrahamsson, Tommy; Almgren, Olle

doi:10.1007/bf01906740

Cited by 30 publications

(11 citation statements)

References 23 publications

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“…Since P-adrenoceptors affect myocardial cAMP levels [11,12], it is not sur prising that cAMP levels are also heteroge neously distributed in the heart. While sym pathetic nerves tend to be localized regionally [ 18,20], we found no significant regional, e.g., EPI versus ENDO, differences in the distribu tion of cAMP.…”

Section: Discussioncontrasting

confidence: 36%

“…The within-animal CV for coronary blood fiow was 12.9% during control condi tions and 20.8% in isoproterenol-stimulated left ventricular myocardium. Approximately half the coronary blood fiow variability can be explained by intrinsic limitations of the radioactive-microsphcre technique [ 1,17], Under control conditions, there is hetero geneity of sympathetic nerve distribution [ 18], tissue norepinephrine content [6,7], and P-adrenoceptors [8,9,19] in the left ventricu lar free wall. Since P-adrenoceptors affect myocardial cAMP levels [11,12], it is not sur prising that cAMP levels are also heteroge neously distributed in the heart.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Isoproterenol on the Relationship between Regional Cyclic AMP and Local Coronary Blood Flow in the Canine Myocardium

Ramirez¹,

Rodriguez²,

Weiss³

1994

Pharmacology

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This study was designed to test in the dog heart the hypothesis that local levels of myocardial cyclic AMP (cAMP) would exhibit significant heterogeneity; that this heterogeneity would correlate with local coronary blood flow, and that this association would be increased with isoproterenol stimulation. Anesthetized open-chest dogs were studied during control (n = 7) or isoproterenol (0.5 µg/kg/min) infusion (n = 7) to test this hypothesis. Coronary blood flow was measured with radioactive microspheres, and cAMP was determined with a competitive binding assay using ³H-cAMP. Significant heterogeneity in cAMP existed during basal [592 pmol/g, within-animal coefficient of variation (100 × mean/SD) = 45.2%] and isoproterenol stimulated [872 (27.3%)] conditions. Method variability could account for only 28% of the total basal variability. No correlation between local blood flow [87 ml/min/ 100 g (12.9%)] and local cAMP was found under control conditions. Both coronary blood flow [206 (20.8%)] and cAMP levels increased significantly with isoproterenol stimulation. There was a significant correlation between normalized stimulated blood flow and cAMP: % flow = 0.39 (% cAMP) + 61.3 (r = 0.48, p < 0.0001). We conclude that significant heterogeneity in myocardial cAMP levels exists, and that this heterogeneity correlates with coronary blood flow heterogeneity during isoproterenol stimulation.

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Section: Discussioncontrasting

confidence: 36%

Section: Discussionmentioning

confidence: 99%

Effect of Isoproterenol on the Relationship between Regional Cyclic AMP and Local Coronary Blood Flow in the Canine Myocardium

Ramirez¹,

Rodriguez²,

Weiss³

1994

Pharmacology

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“…Focal hypertrophy of LV base (Figure 3) in aortic stenosis is a consistent finding with histopathologic data which shows predominant sympathetic innervation of base of the heart [30] [31]. Physiological LVH in the earlier stage of aortic stenosis is transformed to pathologic hypertrophy with development of LV dysfunction [9].…”

Section: Therapeutical Approach To Aortic Stenosissupporting

confidence: 85%

Aortic Stenosis and Stressed Heart Morphology

Karatepe¹,

Yalçın

2014

WJCS

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Myocardial geometric remodeling is a response to increased stress which includes increased afterload situations during clinical conditions. In this review, we have focused on early and late geometric features in aortic stenosis, importance of recognition of these findings and consequences due to progression of valve disease. We have also pointed out the similarities in early focal and global myocardial geometric remodeling in acute and chronic conditions as hypertension and acute stress cardiomypathy which are associated with myocardial functional and geometric response to acute or chronic stress exposure and relevant increased afterload. In aortic stenosis, target organ involvement in disease progression has been evaluated and discussed in the report. In addition to quantitative evaluation of valve disease, importance of myocardial involvement and global assessment of patients with aortic stenosis also have been mentioned in the report. Finally, we have discussed the importance of global myocardial geometric changes and timing for surgery before development of heart failure in this specific group of patients.

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“…There is heterogeneity of sympathetic nerve distribution (Holmgren et al, 1985), tissue noradrenaline content (Petch & Naylor, 1979;Pierpont et al, 1984;Verhoeven et al, 1991), and P-adrenoceptors (Baker et al, 1980;Baker & Sheets, 1982;Brodde et al, 1982;Upsher & Weiss, 1989) in the left ventricular free wall. Sympathetic nerves enter the left ventricle through the basal region of the subepicardium (Angelakos, King & Millard, 1969;Holmgren et al, 1985). While adenylyl cyclase activity tended to be slightly higher in the base than in the apex, there were no significant differences in adenylyl cyclase or flow activity in any region.…”

Section: Discussionmentioning

confidence: 99%

Relationship between local myocardial adenylyl cyclase activity and local coronary blood flow in the dog heart

Rodriguez¹,

Weiss²

1993

Journal of Autonomic Pharmacology

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1. This study tested the hypothesis that heterogeneity of myocardial adenylyl cyclase activity exists and contributes to the heterogeneity of blood flow in canine myocardium, in which flow varies in different areas at any one moment or in a single area at different times. 2. Tissue adenylyl cyclase activity was measured simultaneously with coronary blood flow in the left ventricle of eight anaesthetized open-chest dogs. Coronary flow was measured using radioactive microspheres under control conditions and after sympathetic stimulation. The ventricle was cut into 15 subepicardial and 15 subendocardial sections in which both flow and adenylyl cyclase (basal and forskolin-stimulated) activity were assayed. 3. Control coronary flow was 73.1 +/- 19.9 ml min-1 100 g-1 and ansa subclavia nerve stimulation increased flow to 93.2 +/- 26.1. Baseline adenylyl cyclase activity averaged 51.9 +/- 38.8 pmol min-1 mg protein-1, while forskolin-stimulated activity was 588.2 +/- 393.6. Method variability was approximately 10% of the mean, much less than the biological variability of 70%. 4. Regression analysis of control flow vs baseline adenylyl cyclase activity produced the equation: flow = 0.25 (Adenylyl Cyclase)+60.6, (r = 0.47, P < 0.0001). The equation for stimulated flow vs stimulated adenylyl cyclase was: flow = 0.03 (Adenylyl Cyclase)+73.1, (r = 0.51, P < 0.0001). 5. Thus, significant heterogeneity of tissue adenylyl cyclase activity existed in the heart. There was also a direct linear relationship between adenylyl cyclase activity and coronary flow heterogeneity in canine left ventricle.

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Adrenergic innervation of coronary arteries and ventricular myocardium in the pig: fluorescence microscopic appearance in the normal state and after ischemia

Cited by 30 publications

References 23 publications

Effect of Isoproterenol on the Relationship between Regional Cyclic AMP and Local Coronary Blood Flow in the Canine Myocardium

Effect of Isoproterenol on the Relationship between Regional Cyclic AMP and Local Coronary Blood Flow in the Canine Myocardium

Aortic Stenosis and Stressed Heart Morphology

Relationship between local myocardial adenylyl cyclase activity and local coronary blood flow in the dog heart

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