Adrenaline and Dopamine in Porcine Livers After Cold Preservation With University of Wisconsin Histidine-Tryptophan-Ketoglutarate, Prolactin-Modified Histidine-Tryptophan-Ketoglutarate Solutions

The effect of prolactin concentration on the efficacy of storing isolated porcine kidneys in a modified Biolasol solution

“…The addition of PRL to the HTK solution significantly reduced the leakage of ALT, AST, LDH and gamma-glutamyl transpeptidase (GGTP) from hepatocytes in the rabbit model [23] and the amount of released transaminases, LDH and Ca 2+ , Mg 2+ , Na + , K + ions from hepatocytes in the porcine model [24]. Budziński et al suggest that HTK-PRL affects the stabilization of the cell membrane, minimizing the number of dead hepatocytes [25]. In turn, the addition of human recombinant PRL (rhPRL) to Biolasol reduced the nephron damage markers (pH, osmolarity, K + , Na + , ALT, AST) determined in perfusates collected during reperfusion of isolated porcine kidneys [26].…”

Pharmacological Benefits and Risk of Using Hormones in Organ Perfusion and Preservation Solutions in the Aspect of Minimizing Hepatic Ischemia-Reperfusion Injury during Storage

“…In another study, using the same research model, Budziński et al analysed the influence of PRL on the level of catecholamines after 12 hours of liver storage in HTK + PRL and control solutions. The amount of released dopamine and adrenaline significantly decreased after rinsing grafts with HTK modified with the addition of prolactin, which correlates with the degree of ischemic liver injury [9]. The results obtained in the above publications indicate hepatoprotective properties of prolactin.…”

“…Species differences in liver anatomical structure should also be taken into account. The most similar in terms of size and physiologically similar to human livers are organs derived from domestic pigs, which were used in the model of prolactin [5, 6, 8, 9] and IGF-1/EGF/NGF efficacy [19]. The effects of hormone administration in vivo depend not only on the mammalian species, but also on its sex, age, biopharmaceutical dose and dosage form, time of day and season of its administration, and time of use [129].…”