ADP-ribosylation factor6 regulates both 63H9-noradrenaline and 614C9-glutamate exocytosis through phosphatidylinositol 4,5-bisphosphate

Zheng, Qian; Bobich, Joseph A.

doi:10.1016/j.neuint.2004.03.028

Cited by 16 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the expression of a dominant-negative version of an exchange factor for ARF6 inhibited secretion (12). Similar observations were found in various secreting cell types, including rat brain cortex nerve endings (13). At the same time, an important role for specific lipids, including phosphatidic acid (PA) and phosphatidylinositol 4,5-bisphosphate (PIP 2 ) in membrane fusion was proposed (11,14), but regulation of their biosynthesis remained poorly understood.…”

mentioning

confidence: 57%

See 1 more Smart Citation

ADP Ribosylation Factor 6 (ARF6) Promotes Acrosomal Exocytosis by Modulating Lipid Turnover and Rab3A Activation

Pelletán

Suhaiman

Vaquer

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Sperm acrosomal exocytosis requires GTPases, SNAREs, and a complex lipid signaling. Results: Exocytic stimuli promote ARF6 activation, which accomplishes exocytosis by stimulating PLC and Rab3A. Conclusion: ARF6 induces acrosome calcium efflux and assembles the fusion machinery leading to membrane fusion. Significance: This study explores a novel molecular link between ARF6, PLC, and Rab3A and provides insight into the molecular mechanisms of exocytosis and reproduction.

show abstract

mentioning

confidence: 57%

“…Evidence suggesting its implication in exocytosis comes mostly from studies in neurons and endocrine and neuroendocrine cells, (3,13,62,63). The downstream effector(s) of ARF6 in exocytosis remain uncertain, although linked to the regulation of lipid synthesis.…”

Section: Discussionmentioning

confidence: 99%

ADP Ribosylation Factor 6 (ARF6) Promotes Acrosomal Exocytosis by Modulating Lipid Turnover and Rab3A Activation

Pelletán

Suhaiman

Vaquer

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…15). However, glutamate release from cultured hippocampi was not affected by Arf6 ablation ( Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Trans-regulation of oligodendrocyte myelination by neurons through small GTPase Arf6-regulated secretion of fibroblast growth factor-2

et al. 2014

View full text Add to dashboard Cite

The small G protein ADP-ribosylation factor 6 (Arf6) plays important roles in a wide variety of membrane dynamics-based cellular events such as neurite outgrowth and spine formation in vitro. However, little is known about physiological function of Arf6 in vivo. Here we generate conditional knockout mice lacking Arf6 in neurons, oligodendrocytes, or both cell lineages, and unexpectedly find that Arf6 expression in neurons, but not in oligodendrocytes, is crucial for oligodendrocyte myelination in the hippocampal fimbria and the corpus callosum during development, and that this is through the regulation of secretion of fibroblast growth factor-2, a guidance factor for migration of oligodendrocyte precursor cells (OPCs). These results suggest that Arf6 in neurons plays an important role in OPC migration through regulation of FGF-2 secretion during neuronal development.

show abstract

“…It is well‐established that Arf6 participates in membrane trafficking including endocytosis, post‐endocytic recycling and exocytosis, lipid rafts dynamics and reorganization of plasma membrane structure [Donaldson, 2003; D'Souza‐Schorey and Chavrier, 2006]. Arf6 regulates actin cytoskeleton remodeling, cell spreading, and cell adhesions disassembly [Schafer et al, 2000; Palacios et al, 2001], as well as phospholipids metabolism [Honda et al, 1999; Aikawa and Martin, 2003; Lawrence and Birnbaum, 2003; Zheng and Bobich, 2004]. Over the past few years Arf6 draws more and more attention in the aspect of carcinogenesis and tumor progression via participation in growth factor receptors turnover [D'Souza‐Schorey et al, 1995] and stimulation of cell migration and invasion [Hashimoto et al, 2004; Tague et al, 2004; Li et al, 2006b; Sabe et al, 2009].…”

mentioning

confidence: 99%

Arf6 promotes cell proliferation via the PLD‐mTORC1 and p38MAPK pathways

Knizhnik¹,

Ковалева²,

Komelkov³

et al. 2011

J of Cellular Biochemistry

View full text Add to dashboard Cite

The small G-protein ADP-ribosylation factor 6 (Arf6) belongs to the Ras GTPases superfamily and is mostly known for its actin remodeling functions and involvement in the processes of plasma membrane reorganization and vesicular transport. The majority of data indicates that Arf6 contributes to cancer progression through activation of cell motility and invasion. Alternatively, we found that the expression of a wild-type or a constitutively active Arf6 does not influence tumor cell motility and invasion but instead significantly stimulates cell proliferation and activates phospholipase D (PLD). Conversely the expression of a mutant Arf6 (Arf6N48I), that is, unable to interact with PLD has no effect on proliferation but promotes motility, invasion, and matrix degradation by uPA extracellular proteinase. Studying the mechanisms of Arf6-dependent stimulation of cell proliferation, we found some signaling pathways contributing to Arf6 promitogenic activity. Namely, we showed that Arf6 in a PLD-mTORC1-dependent manner activates S6K1 kinase, a well-known regulator of mitogen-stimulated translation initiation. Furthermore, we demonstrated an Arf6-dependent phosphorylation of mTORC1 downstream targets, 4E-BP1 and ribosomal S6 protein, confirming an existence of Arf6-PLD-mTORC1-S6K1/4E-BP1 signaling pathway and also demonstrated its impact on proliferation stimulation. Next, we found that Arf6 activation potentiates Erk1/2 and p38MAP kinases phosphorylation. Surprisingly, p38 opposite to Erk1/2 significantly contributes to Arf6-dependent proliferation increase promoting S6 ribosomal protein phosphorylation at Ser235/236 residues. Therefore, we demonstrated Arf6 proliferation stimulating activity and revealed PLD-mTORC1 and p38MAP kinase as Arf6 partners mediating promitogenic activity. These results highlight a new aspect of Arf6 functioning in cancer cell biology.

show abstract

ADP-ribosylation factor6 regulates both 63H9-noradrenaline and 614C9-glutamate exocytosis through phosphatidylinositol 4,5-bisphosphate

Cited by 16 publications

References 50 publications

ADP Ribosylation Factor 6 (ARF6) Promotes Acrosomal Exocytosis by Modulating Lipid Turnover and Rab3A Activation

ADP Ribosylation Factor 6 (ARF6) Promotes Acrosomal Exocytosis by Modulating Lipid Turnover and Rab3A Activation

Trans-regulation of oligodendrocyte myelination by neurons through small GTPase Arf6-regulated secretion of fibroblast growth factor-2

Arf6 promotes cell proliferation via the PLD‐mTORC1 and p38MAPK pathways

Contact Info

Product

Resources

About