ADP-glucose pyrophosphorylase activity in relation to yield potential of wheat: Response to independent and combined high temperature and drought stress

Kaur, Vikender; Madaan, Sukham; Behl, R. K.

doi:10.1556/0806.45.2017.003

Cited by 12 publications

(12 citation statements)

References 30 publications

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“…Grain starch synthesis was mainly regulated by AGPase, SSS, and SBE, the activities of which were reduced by heat stress. Similar results were reported in normal maize 5 , 6 , 20 , wheat 15 – 17 , and rice 11 , 32 . However, the starch content before 20 DAP was increased, and starch accumulation per grain was not affected by heat stress, which was inconsistent with the decreased activities of AGPase, SSS, and SBE.…”

Section: Discussionsupporting

confidence: 87%

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Heat stress during grain filling affects activities of enzymes involved in grain protein and starch synthesis in waxy maize

Yang

Ding

et al. 2018

Sci Rep

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High temperature (temperature over 35 °C) is an extremely important environmental factor that affects the maize grain quality in Southern China. The effects of heat stress after pollination on grain protein and starch deposition and activities of involved enzymes were studied in a pot trail in 2014 and 2015. Results showed that grain dry weight reductions at maturity were 19.8% and 19.1%, whereas starch contents (mg g−1) were reduced by 3.0% and 3.3%, and starch accumulation (mg grain−1) were reduced 22.2% and 21.8% in 2014 and 2015, respectively. Protein content was decreased by heat stress before 15 DAP and increased thereafter. At maturity, protein contents (mg g−1) were increased by 24.5% and 25.3% in 2014 and 2015, while protein accumulation (mg grain−1) were not affected by heat stress. In response to heat stress, glutamate synthase activity was enhanced by 29.1–82.9% in 2014 and 2.0–141.8% in 2015, whereas glutamine synthetase activity was reduced by 1.9–43.5% in 2014 and 0.1–27.4% in 2015 throughout the grain filling. The activities of sucrose phosphate synthase were decreased by heat stress at 10–25DAP (12.7–32.0%) in 2014 and 15–20 DAP (23.2–27.5%) in 2015, and activities of sucrose synthase were decreased by heat stress at 5–15 DAP (20.0–45.0%) in 2014 and 15 DAP (22.0%) in 2015, repectively. The activities of enyzmes that involved in starch synthessis were all suppressed by heat stress during grain filling, and the reduction of adenosine diphosphate-glucose pyrophosphorylase, soluble starch synthase, and starch branching enzyme were decreased by 21.3–43.1%, 19.1–29.2%, and 7.0–45.6% in 2014 and 1.8–78.5%, 21.4–51.2%, and 11.0–48.0% in 2015, respectively. Conclusively, grain weight and starch deposition were suppressed by heat stress due to the decreased activities of enzymes involved in starch synthesis, and the increased protein content was due to the enhanced activity of glutamate synthase.

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Section: Discussionsupporting

confidence: 87%

“…In wheat, SuSy in grain was enhanced before 14 DAP and reduced thereafter 15 . The low starch under heat stress may be due to the decreased activities of SSS 16 and AGPase 17 .…”

Section: Introductionmentioning

confidence: 99%

Heat stress during grain filling affects activities of enzymes involved in grain protein and starch synthesis in waxy maize

Yang

Ding

et al. 2018

Sci Rep

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“…Likewise the activity of adenosine diphosphate-glucose pyrophosphorylase (AGPase) is associated with grain filling [27]. Under drought stress, a positive correlation of AGPase with developing grains is reported in wheat and rice [15] [28], while a decrease in AGPase activity was reported in sensitive genotypes of wheat [29].…”

Section: Introductionmentioning

confidence: 99%

Activities of leaf and spike carbohydrate-metabolic and antioxidant enzymes are linked with yield performance in three spring wheat genotypes grown under well-watered and drought conditions

et al. 2020

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Background: To improve our understanding about the physiological mechanism of grain yield reduction at anthesis, three spring wheat genotypes [L 1 (advanced line), L 2 (Vorobey) and L 3 (Punjab-11)] having contrasting yield potential under drought in field were investigated under controlled greenhouse conditions, drought stress was imposed at anthesis stage by withholding irrigation until all plant available water was depleted, while wellwatered control plants were kept at 95% pot water holding capacity. Results: Compared to genotype L 1 and L 2 , pronounced decrease in grain number (NGS), grain yield (GY) and harvest index (HI) were found in genotype L 3 , mainly due to its greater kernel abortion (KA) under drought. A significant positive correlation of leaf monodehydroascorbate reductase (MDHAR) with both NGS and HI was observed. In contrast, significant negative correlations of glutathione S-transferase (GST) and vacuolar invertase (vacInv) both within source and sink were found with NGS and HI. Likewise, a significant negative correlation of leaf abscisic acid (ABA) with NGS was noticed. Moreover, leaf aldolase and cell wall peroxidase (cwPOX) activities were significantly and positively associated with thousand kernel weight (TKW). Conclusion: Distinct physiological markers correlating with yield traits and higher activity of leaf aldolase and cwPOX may be chosen as predictive biomarkers for higher TKW. Also, higher activity of MDHAR within the leaf can be selected as a predictive biomarker for higher NGS in wheat under drought. Whereas, lower activity of vacInv and GST both within leaf and spike can be selected as biomarkers for higher NGS and HI. The results highlighted the role of antioxidant and carbohydrate-metabolic enzymes in the modulation of source-sink balance in wheat crops, which could be used as bio-signatures for breeding and selection of drought-resilient wheat genotypes for a future drier climate.

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“…Negative correlations of TKW with spike UGPase, PGM and PFK were also found under drought conditions ( Table 7). No supporting literature is available to con rm the results of the present study however, AGPase is reported to have positive correlation with grain llings [27] [29]. Maize and rice transgenes having Shrunken2 gene (Sh2r6hs), which encodes an altered AGPase activity showed increased the biomass and seed weight [71] [72].…”

Section: Correlation Of Carbohydrate Catalyzing Enzymes With Tkwmentioning

confidence: 43%

“…Likewise the activity of adenosine diphosphate-glucose pyrophosphorylase (AGPase) is associated with grain lling [27]. Under drought stress, a positive correlation of AGPase with developing grains is reported in wheat and rice [15] [28], while a decrease in AGPase activity was reported in sensitive genotypes of wheat [29].…”

Section: Introductionmentioning

confidence: 99%

Higher activity of monodehydroascorbate reductase and lower activities of leaf and spike vacuolar invertase and glutathione S-transferase reveals higher number of grains per spike in spring wheat genotypes grown under well-watered and drought conditions

Shokat

Großkinsky

Roitsch

et al. 2020

Preprint

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Background: To improve our understanding about the physiological mechanism of grain yield reduction at anthesis, three spring wheat genotypes (L1, L2 and L3) having contrasting yield potential under drought in field were investigated under controlled greenhouse conditions., drought stress was imposed at anthesis stage by withholding irrigation until all plant available water was depleted, while well-watered control plants were kept at 95% pot water holding capacity. Results: Compared to genotype L1 and L2, pronounced decrease in grain number (NGS), grain yield (GY) and harvest index (HI) were found in genotype L3, mainly due to its greater kernel abortion (KA) under drought. A significant positive correlation of leaf monodehydroascorbate reductase (MDHAR) with both NGS and HI was observed. In contrast, significant negative correlations of glutathione S-transferase (GST) and vacuolar invertase (vacInv) both within source and sink with NGS and HI were found. Likewise, a significant negative correlation of leaf abscisic acid (ABA) with NGS was noticed. Moreover, leaf aldolase and cell wall peroxidase (cwPOX) activities were significantly and positively associated with TKW. Conclusion: Collectively, distinct physiological markers were correlating with yield traits and higher activity of leaf aldolase and cwPOX may be chosen as predictive bio-markers for higher TKW. Also, higher activity of MDHAR within the leaf can be selected as a predictive bio-marker for higher NGS in wheat under drought. Whereas, lower activity of vacInv and GST both within leaf and spike can be selected as bio-markers for higher NGS and HI. The results highlighted the role of antioxidant and carbohydrate-metabolic enzymes in the modulation of source-sink balance in wheat crops, which could be used as bio-signatures for breeding and selection of drought-resilient wheat genotypes for a future drier climate.

show abstract

ADP-glucose pyrophosphorylase activity in relation to yield potential of wheat: Response to independent and combined high temperature and drought stress

Cited by 12 publications

References 30 publications

Heat stress during grain filling affects activities of enzymes involved in grain protein and starch synthesis in waxy maize

Heat stress during grain filling affects activities of enzymes involved in grain protein and starch synthesis in waxy maize

Activities of leaf and spike carbohydrate-metabolic and antioxidant enzymes are linked with yield performance in three spring wheat genotypes grown under well-watered and drought conditions

Higher activity of monodehydroascorbate reductase and lower activities of leaf and spike vacuolar invertase and glutathione S-transferase reveals higher number of grains per spike in spring wheat genotypes grown under well-watered and drought conditions

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