Adorno’s Kinderjahr

Design of experiment (DoE)‐driven in vitro and in vivo uptake studies of exosomes for pancreatic cancer delivery enabled by copper‐free click chemistry‐based labelling

“…This finding also highlights the importance of assessing the expression of CD47 on exosomes as a selection criteria for suitability of a particular exosome type to be used as in vivo drug nanocarriers. The preferential uptake of unmodified self-exosomes by PC cells is therefore desirable for applications as nanocarriers for therapeutic delivery against PC, but warrants investigation into the risk of such strategy being counter-productive in terms of treatment efficacy due to the implication of tumour-derived exosomes in promoting tumour progression and metastasis [ 43 ]. Treatment efficacy in pre-clinical PC models using PC-derived exosomes as nanocarriers should also be correlated with the extent of tumour accumulation of the unmodified exosomes, to determine if endowment of active targeting ligands specific for certain surface proteins overexpressed in PC (e.g.…”

Anti‐CD63‐Oligonucleotide Functionalized Magnetic Beads for the Rapid Isolation of Small Extracellular Vesicles and Detection of EpCAM and HER2 Membrane Receptors using DARPin Probes

“…[11] For example, it was shown that release of sEVs from cancer cells is higher compared to normal cells. [12] Thus, isolation of distinct sEVs secreted by cancer, or other abnormal cells followed by the identification of their surfaceprotein markers and cargo could substantially contribute to the development of assays for detection of different diseases at the early stages. In this regard, the capture of sEV subpopulations and identification of their surface-protein markers prior to the analysis of their cargo, such as genetic biomarkers, are valuable steps that comprise the sEV characterization pipeline.…”

“…Like circulating tumor cells (CTC), circulating tumor DNA (ctDNA) and other biomarkers, sEVs represent another valuable biomarker for “liquid biopsy” capable of enhancing the accuracy of diagnosis or the post‐screening procedure [11] . For example, it was shown that release of sEVs from cancer cells is higher compared to normal cells [12] . Thus, isolation of distinct sEVs secreted by cancer, or other abnormal cells followed by the identification of their surface‐protein markers and cargo could substantially contribute to the development of assays for detection of different diseases at the early stages.…”

Simultaneous isolation and preconcentration of exosomes by ion concentration polarization

“…Furthermore, the majority of cell‐free miRNAs reside within exosomes . This, combined with the fact that the proteins and RNA they carry are specific to the cell of origin, potentially make exosomes and their cargo excellent noninvasive biomarkers . One example of such an exosomal biomarker is miRNA‐141, which occurs in prostate cancer patients at expression levels four times higher than that in healthy patients .…”