Adoptive infusion of tolerance dendritic cells prolongs survival of small intestine allografts in rats: systematic review and meta‐analysis

Abstract

Our results demonstrated that Tol-DCs induced by four methods prolong rat small intestine allograft survival. Intravenous infusion of 5×10(6) to 6×10(6) Tol-DCs was the optimum dose in rat small intestine transplantation. Immunosuppressive or costimulatory blockade was synergistic with Tol-DC on graft survival. Additional high-quality studies with larger sample sizes are needed to better investigate small intestinal graft longer term survival.

Effects of Adoptive Transfer of Tolerogenic Dendritic Cells on Allograft Survival in Organ Transplantation Models: An Overview of Systematic Reviews

“…Screening by the titles and abstracts, we excluded 1027 articles for irrelevant themes or unwanted article types and 7 were selected to be read in their entirety. Of those, 1 systematic review was excluded for irrelevant theme and 6 systematic reviews assessing the efficacy of Tol-DC treatment in animal models of heart, liver, kidney, small intestine, skin, and islet transplantation satisfied our inclusion and exclusion criteria and were further evaluated ( Figure 1 ) [ 10 – 15 ]. Of the 112 studies included in the six systematic reviews, 65 studies were excluded because of inadequate data for meta-analysis (heart 28, skin 16, kidney 9, islet 8, small intestine 3, and liver 2), and the remaining 47 studies were included in our overview [ 8 , 16 , 17 , 19 – 63 ].…”

“…Of the six included reviews, which were published between 2012 and 2014, only one conducted a meta-analysis [ 10 – 15 ]. The remaining five had incomplete information, such as omission of sample size or standard deviation, and applied semiquantitative methods to analyze the collected data.…”

Does carbon dioxide pneumoperitoneum enhance wound metastases following laparoscopic abdominal tumor surgery? A meta-analysis of 20 randomized control studies

“…Jada Queries or Cochrane tools are the most commonly used power tools for this purpose; however, the subjects in those studies were humans. In our meta-analysis, the subjects were animals, so they did not follow the assessment methods in the Jada Queries or Cochrane tools guidelines, such as “double blinding.” We used the method described by Sun et al to evaluate the study quality [19], which may have produced bias. Four, substantial heterogeneity was present among the studies.…”

“…We used the method described by Sun et al to evaluate the quality of the involved studies [19]. The study quality was rated using the following six criteria [20, 21]: (I) peer-reviewed publication (score of 2); (II) random allocation to treatment or control (score of 2); (III) animal species (inbred strain, age-matched, statement of MHC mismatch, score of 2); (IV) sample size (sample sizes of both the control and experimental groups must be clearly defined; score of 1); (V) animal welfare regulations were observed (score of 1); and (VI) statement of potential conflict of interests (funding sources must be clearly stated; score of 1).…”

Clinical Aspects of Regenerative Medicine

“…This latter function is attributed to immature dendritic cells, which unlike their mature counterparts, are capable of eliminating T cell responses through deletion or through the generation of T regulatory cells, which can in turn suppress other T and B cell responses [14]. In two meta-analyses of experimental rodent studies using infusions of tolerogenic dendritic cells in islets or intestinal grafts [15,16], four methods of production were identified: selection of immature dendritic cells, gene modification, cytokine-induced, or drug-treated. A single dose appeared to be equal or superior to multiple doses.…”