AdoP assay detection of mycoplasmal contamination in biological media

Bonissol, C; Stoiljkovic, Bob

doi:10.1016/s0923-2516(89)80101-3

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…Identity of ECs was confirmed by a positive staining with a monoclonal antibody for von Willebrand factor. Absence of mycoplasma contamination was checked by using the enzymatic ADOP assay 27…”

Section: Methodsmentioning

confidence: 99%

Heparin and non‐heparin‐like dextrans differentially modulate endothelial cell proliferation: In vitro evaluation with soluble and crosslinked polysaccharide matrices

Letourneur

Machy

Pellé

et al. 2002

J. Biomed. Mater. Res.

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Proliferation of endothelial cells (ECs) is a cellular step of particular importance for implanted cardiovascular biomaterials. Heparin and some synthetic water-soluble non-anticoagulant polysaccharides derived from dextran and bearing anionic carboxymethyl and hydrophobic benzylamine groups were first investigated for their effects on EC proliferation in vitro. The results assessed by cell counting, 3H-thymidine uptake, and flow cytometry analysis, showed that the derivatized dextran-bearing hydrophobic groups stimulated the EC growth in the presence of serum, whereas native dextran or dextran-bearing anionic carboxymethyl groups were inactive and heparin was slightly inhibitory. Then, we showed that the derivatized dextran enhanced EC proliferation by potentiation of the mitogenic activities of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2), two potent EC growth factors. In the presence of 2 nM of derivatized dextran, a 3-fold and 13-fold increase of 3H-thymidine uptake was obtained with VEGF and FGF-2, respectively. Finally, proliferation of ECs was investigated on crosslinked gels made of polysaccharides. It is of interest that EC proliferation was higher on gels containing the derivatized dextran than on plain hydrogels, and heparinized gels inhibited cell proliferation. From the obtained results, we propose that the synthetic non-heparin-like dextran may be of interest as a coating for the endothelialization of cardiovascular biomaterials.

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Section: Methodsmentioning

confidence: 99%

Heparin and non‐heparin‐like dextrans differentially modulate endothelial cell proliferation: In vitro evaluation with soluble and crosslinked polysaccharide matrices

Letourneur

Machy

Pellé

et al. 2002

J. Biomed. Mater. Res.

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“…An enzyme, that has never been detected in mammalian cells, but which seems to be commonly present in a wide variety of mycoplasmas, is adenosine phosphorylase, which catalyses the reversible conversion of adenosine (or deoxyadenosine) to adenine and (2Ľ -deoxy)ribose-1-phosphate. 38,39 This enzyme activity was not noted in M pneumoniae and Mycoplasma lipophilium (the latter mycoplasmas have the ability to convert adenosine into inosine by deamination). M orale infections of murine L1210 and P388 leukaemia cells were shown to change intracellular deoxyadenosine metabolism.…”

Section: Personal Viewmentioning

confidence: 94%

Improvement of purine and pyrimidine antimetabolite-based anticancer treatment by selective suppression of mycoplasma-encoded catabolic enzymes

Liekens

Bronckaers

Balzarini

2009

The Lancet Oncology

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Most mycoplasmas are present as commensals, colonising the mucosa of our respiratory and gastrointestinal tract. Experimental data suggest that the long-term association of certain mycoplasma species with mammalian cells might favour host-cell transformation and malignancy. Moreover, increased mycoplasma infection has been noted in several cancers. Despite efforts to develop target-specific anticancer drugs, current cancer treatment still relies on the use of nucleobase or nucleoside-based analogues. Here, we provide experimental evidence that nucleoside-metabolising catabolic enzymes expressed by mycoplasmas substantially compromise the efficacy of nucleoside antimetabolites used in the treatment of cancer. We also suggest potential methods for improving future chemotherapy by suppressing mycoplasma-mediated catabolism of the anticancer nucleoside analogues.

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Detection of mycoplasma contaminations by the polymerase chain reaction

Wirth¹,

Berthold

Grashoff³

et al. 1994

Cytotechnology

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The polymerase chain reaction (PCR) has been used for the general detection of Mollicutes. 25 Mycoplasma and Acholeplasma species were detected including important contaminants of cell cultures such as M. orale, M. arginini, M. hyorhinis, M. fermentans, A. laidlawii and additional human and animal mycoplasmas. PCR reactions were performed using a set of nested primers defined from conserved regions of the 16S rRNA gene. The detection limit was determined to be 1 fg mycoplasma DNA, which is equivalent to 1-2 genome copies of the 16S rRNA coding region. The identity of the amplification products was confirmed by agarose gel electrophoresis and restriction enzyme analysis. DNA from closely and distantly related micro-organisms did not give rise to specific amplification products. The method presented here offers a much more sensitive, specific and rapid assay for the detection of mycoplasmas than the existing ones.

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AdoP assay detection of mycoplasmal contamination in biological media

Cited by 6 publications

References 24 publications

Heparin and non‐heparin‐like dextrans differentially modulate endothelial cell proliferation: In vitro evaluation with soluble and crosslinked polysaccharide matrices

Heparin and non‐heparin‐like dextrans differentially modulate endothelial cell proliferation: In vitro evaluation with soluble and crosslinked polysaccharide matrices

Improvement of purine and pyrimidine antimetabolite-based anticancer treatment by selective suppression of mycoplasma-encoded catabolic enzymes

Detection of mycoplasma contaminations by the polymerase chain reaction

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