Administration of nerve growth factor-β to heifers with a pre-ovulatory follicle enhanced luteal formation and function and promoted LH release

Stewart, Jamie L.; Stella, Stephanie; Cunha, L.L.; Dias, Nicholas W; Canisso, Igor F.; Mercadante, Vitor R G; Cardoso, Rodolfo C.; Williams, Gary L.; Pohler, Ky G; Lima, F.S.

doi:10.1016/j.theriogenology.2020.02.040

Cited by 9 publications

(11 citation statements)

References 47 publications

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“…Administration of NGF may mimic the endogenous uptake of SPP occurring at copulation. Our data suggest that NGF is augmenting progesterone in a manner that resembles the physiologi-cal increase reported in beef and dairy cattle (Stewart et al, 2018(Stewart et al, , 2020. However, it is also important to note that the administration of 1 mg of NGF did not induce ovulation in pre-pubertal heifers (Tanco et al, 2012).…”

Section: Introductionsupporting

confidence: 75%

“…In camelids, NGF appears to play a critical role in ovulation (Kershaw-Young et al, 2012). In addition, NGF has been shown to increase the release of LH from the anterior pituitary and may have direct ovarian effects in camelids with a ≥8-mm follicle when treated intramuscularly with 1.0 mg of NGF (Silva et al, 2012) and in cattle with a pre-ovulatory follicle when treated intramuscularly with 250 µg (Stewart et al, 2020). The role of NGF in species other than camelids has not been well explored; although limited, previ-ous work demonstrated that purified NGF from bull seminal plasma injected at the time of AI increased circulating progesterone, increased relative abundance of interferon-stimulated genes (ISG), and increased pregnancy-associated glycoprotein concentration at d 24 and 28 post-AI in synchronized beef cows (Stewart et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effect of nerve growth factor-β administered at insemination for lactating Holstein dairy cows bred after timed-artificial insemination protocol

Hubner

Canisso

Peixoto

et al. 2022

Journal of Dairy Science

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The objectives of this study were to determine the effects of nerve growth factor-β (NGF), purified from bulls' seminal plasma and administered at the time of artificial insemination (AI), on progesterone post-AI, interferon-stimulated genes (ISG), and pregnancy per AI (P/AI) for lactating Holstein dairy cows enrolled in a timed-AI protocol. We hypothesized that administration of NGF at the time of AI would increase plasma progesterone post-AI, upregulate relative abundance of ISG, and improve P/AI in lactating dairy cows. Holstein cows (n = 557) from a single commercial dairy farm were blocked by parity and randomly assigned to receive an intramuscular injection containing 296 µg of bovine purified NGF at the time of AI, diluted in 2 mL of phosphate-buffered saline (NGF: n = 275), or receive only the 2 mL of phosphate-buffered saline (control: n = 282). Plasma progesterone and corpus luteum size were assessed in a subset of cows (NGF: n = 32; control: n = 36) at d 7, 14, and 19 post-AI. Relative mRNA abundance of ISG (ISG15, MX1, MX2, and RTP4) was assessed in peripheral blood leukocytes on d 19 post-AI. Pregnancy diagnosis was performed at 37 and 65 d post-AI. There was an interaction effect between treatment and parity for plasma progesterone; however, plasma progesterone and ISG did not differ between treatments. There were no effects of NGF for P/AI at 37 d post-AI (NGF = 40.0% vs. control = 41.6%), 65 d post-AI (NGF = 36.0% vs. control = 38.1%), and for pregnancy loss (NGF = 8.4% vs. control = 7.7%). The current study revealed that effects to NGF in lactating Holstein cows were minor and contingent with parity for progesterone, and no improvement in ISG relative abundance and P/AI were observed.

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Section: Introductionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Effect of nerve growth factor-β administered at insemination for lactating Holstein dairy cows bred after timed-artificial insemination protocol

Hubner

Canisso

Peixoto

et al. 2022

Journal of Dairy Science

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“…Though we found no differences in expression of LHCGR in the current study, it would be worthwhile to evaluate its expression in vivo to determine if there are downstream effects on the presence of small luteal cells in the mature bovine CL. This nding may explain how systemic administration of NGF can improve CL development and function in cattle (12,14,15,41).…”

Section: Discussionmentioning

confidence: 88%

“…Our understanding of the role of NGF in promoting mammalian ovulation became even more complex when it was identi ed as the ovulation-inducing factor in camelid seminal plasma (10,11). Though the ability of the ovary to produce NGF in spontaneously ovulating species is well documented, few studies have evaluated whether the introduction of NGF from seminal plasma at time of breeding may have a role within the bovine hypothalamic-pituitary-ovarian axis (12)(13)(14)(15). In camelids, intrauterine absorption of seminal plasma NGF into systemic circulation occurs within 15 min of copulation, after which it stimulates the preovulatory LH peak from the anterior pituitary gland and exerts a dose-dependent luteotropic effect on the developing corpus luteum (CL) (16).…”

Section: Introductionmentioning

confidence: 99%

“…In camelids, intrauterine absorption of seminal plasma NGF into systemic circulation occurs within 15 min of copulation, after which it stimulates the preovulatory LH peak from the anterior pituitary gland and exerts a dose-dependent luteotropic effect on the developing corpus luteum (CL) (16). Studies have shown that NGF retains its luteotropic properties when administered systemically to cattle (12)(13)(14). While the luteotropic effect in camelids is attributed to the prolonged LH secretion from the pituitary, in cattle, there is evidence that NGF from the seminal plasma may act directly on the ovary (5).…”

Section: Introductionmentioning

confidence: 99%

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Direct Effects of Nerve Growth Factor-β, Purified From Bull Seminal Plasma,on Steroidogenesis and Angiogenic Markers of Thebovine Pre-ovulatory Follicle

Stewart

Gao

Flaws

et al. 2020

Preprint

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Nerve growth factor-β (NGF) is critical for ovulation in the mammalian ovary and is luteotrophic when administered systemically to camelids and cattle. This study aimed to assess the direct effects of purified bovine NGF on steroidogenesis and angiogenic markers in the bovine pre-ovulatory follicle. A chort of Holstein heifers were synchronized with a standard protocol and heifers with the preovulatory follicle (≥ 12 mm) had an the ovary containing the dominant follicle removed via colpotomy. Pre-ovulatory follicles were dissected in 24 pieces containing theca and granulosa cells that were randomly allocated to receive either cultured in media supplemented with purified bovine NGF (100 ng/mL) or untreated (control) for 72 h. The supernatant media was harvested for determination of progesterone, testosterone, and estradiol, whereas explants were used for mRNA analyses for steroidogenesis and angiogenic markers. Treatment of follicle tissue with NGF upregulated gene expression of steroidogenic enzyme HDS17B ( P = 0.04) and increased testosterone production ( P < 0.01). However, NGF treatment did not alter production of progesterone ( P = 0.81) or estradiol ( P = 0.14). Consistently, gene expression of steroidogenic enzymes responsible for producing these hormones ( STAR , CYP11A1 , HSD3B , CYP17A1 , CYP19A1 ) were unaffected by NGF treatment ( P ≥ 0.31). Treatment with NGF downregulated gene expression of the angiogenic enzyme FGF2 ( P = 0.02) but did not alter PGES ( P = 0.63), VEGFA ( P = 0.44), and ESR1 ( P = 0.77). Collectively, these results demonstrate that NGF from seminal plasma may interact directly with the bovine pre-ovulatory follicle to alter downstream steroidogenesis and luteal development.

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Review: Unveiling the effect of beta-nerve growth factor on the reproductive function in llamas and cows

Ratto

Paiva

Carrasco

et al. 2023

animal

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Administration of nerve growth factor-β to heifers with a pre-ovulatory follicle enhanced luteal formation and function and promoted LH release

Cited by 9 publications

References 47 publications

Effect of nerve growth factor-β administered at insemination for lactating Holstein dairy cows bred after timed-artificial insemination protocol

Effect of nerve growth factor-β administered at insemination for lactating Holstein dairy cows bred after timed-artificial insemination protocol

Direct Effects of Nerve Growth Factor-β, Purified From Bull Seminal Plasma,on Steroidogenesis and Angiogenic Markers of Thebovine Pre-ovulatory Follicle

Review: Unveiling the effect of beta-nerve growth factor on the reproductive function in llamas and cows

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