Administration of MIP-3α gene to the tumor following radiation therapy boosts anti-tumor immunity in a murine model of lung carcinoma

Zhu, Bo; Zou, Liping; Cheng, Xiaoming; Lin, Zhihua; Duan, Yu-zhong; Wu, Yuzhang; Zhou, Fan; Chen, Zhengtang

doi:10.1016/j.imlet.2005.09.010

Cited by 9 publications

(8 citation statements)

References 23 publications

(33 reference statements)

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“…These 'danger signals' (such as TNF-a and IL-1) attract the DCs to the tumor site resulting in an increased number of the DCs at the tumor site. 30 In our study, we confirmed that sole CpG ODNs (class C) applied three times are capable of delaying the growth of subcutaneous B16F1 tumors, which is in agreement with the results obtained with the class B CpG ODNs in various mouse tumor models. [19][20][21][22] Yet, we observed no dose-dependent response even though the tumor growth was most effectively delayed in the group treated with the highest dose of CpG ODNs (90 mg).…”

Section: Discussionsupporting

confidence: 91%

“…On the other hand, the damage to the tumor tissue caused by irradiation induces the production of pro-inflammatory cytokines (TNF-a, IL-1) and chemokines that attract the DCs from the blood flow to the tumor site. 30 On the periphery of the tumor (where tumor vascularity is still functional), consequently the number of DCs increases after irradiation. We believe that the combination of irradiation with peritumorally applied CpG ODNs in this way enhances the antitumor effect.…”

Section: Discussionmentioning

confidence: 99%

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Class C CpG oligodeoxynucleotides as a single agent and in combination with radiotherapy efficiently delayed growth of subcutaneous B16F1 tumors

et al. 2009

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Until now, the anti-tumor efficacy of synthetic oligodeoxynucleotides containing CpG motifs (CpG ODNs) has been reported in a number of preventive and therapeutic tumor models. Predominately class B CpG ODNs were used, relatively little has been reported regarding the class C CpG ODNs. The present study was, therefore, aimed at assessing the ability of CpG ODNs class C applied as a single agent and in combination with radiotherapy to induce the anti-tumor immunity in an experimental tumor model in mice (subcutaneous [s.c.] B16F1). Class C CpG ODNs applied three times as a single agent efficiently delayed the growth of s.c. B16F1 tumors. The combined therapy (CpG ODNs and tumor irradiation) remarkably enhanced the anti-tumor effect. The peritumoral (p.t.) application of CpG ODNs in combination with irradiation increased the number of dendritic cells (DCs) at the tumor site and improved the antigen loading and maturation of DCs. In conclusion, the combined therapy with CpG ODNs and irradiation creates a unique in situ DCs vaccine that could be easily applicable without prior knowledge of tumor antigens.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Class C CpG oligodeoxynucleotides as a single agent and in combination with radiotherapy efficiently delayed growth of subcutaneous B16F1 tumors

et al. 2009

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“…24,25 DCs recruited into epithelial tissues through CCR6/MIP-3a interaction are responsible for cross-priming of CD8 + T-cell in vivo. 26 It has been demonstrated that crosspriming is a critical event in the presentation of tumor antigens.…”

Section: Discussionmentioning

confidence: 99%

Conditioning Vaccination Site With Irradiated MIP-3α–transfected Tumor Cells Enhances Efficacy of Dendritic Cell-based Cancer Vaccine

Shih

Yang

Cheng

et al. 2009

Journal of Immunotherapy

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Macrophage inflammation protein-3alpha (MIP-3alpha) is a chemokine expressed in inflamed tissue and capable of inducing migration of immature dendritic cells (DCs) or Langerhans cells. We postulated that conditioning vaccination sites with MIP-3alpha might enhance the efficacy of subsequently administered DC-based cancer vaccines. Our results demonstrate that subcutaneously injection of irradiated tumor cells expressing MIP-3alpha induces substantial cell infiltration to the injection site. Vaccination of irradiated tumor cells expressing MIP-3alpha followed by DCs pulsed with irradiated tumor cells can effectively suppress tumor growth in animals, which is significantly better than vaccination with irradiated MIP-3alpha-producing tumor cells or DCs pulsed with tumor cells alone. The protective effect was most evident when the MIP-3alpha-producing tumor cells and DC-based vaccines were injected at the same site. These results support the notion that this combination vaccination strategy might generate a more effective immune response to suppress the growth of tumor cells in animals.

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“…Costimulatory molecules as potential targets significantly contribute to the therapeutic intervention in allergic airway disease [36–38]. Targeting of antigens to DC surface receptors elicits effective immune responses [39,40].Combination vaccination has better therapeutic effects on antigen‐associated inflammation [41,42].…”

Section: Discussionmentioning

confidence: 99%

Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation

Wang

Qian

Wang

et al. 2008

Clinical and Experimental Immunology

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SummaryVaccination with allergen-encoding DNA has been proposed as having potential for allergen-specific immunotherapy. In this study, we examine the therapeutic effect of allergen-encoding DNA vaccination directly to dendritic cells ( + CD80 + and CD11c + CD86 + on pulmonary DCs, as compared with animals with OVA-pcDNA3·1, pcDNA3·1 and OVA respectively. DNA vaccine encoding both Fc and OVA was shown to be more effective than DNA vaccine encoding OVA alone. Our data indicate that Fc-antigen combinationencoding DNA vaccination has better preventive effects on antigen-induced airway inflammation by regulating DCs, and may be a new alternative therapy for asthma.

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Administration of MIP-3α gene to the tumor following radiation therapy boosts anti-tumor immunity in a murine model of lung carcinoma

Cited by 9 publications

References 23 publications

Class C CpG oligodeoxynucleotides as a single agent and in combination with radiotherapy efficiently delayed growth of subcutaneous B16F1 tumors

Class C CpG oligodeoxynucleotides as a single agent and in combination with radiotherapy efficiently delayed growth of subcutaneous B16F1 tumors

Conditioning Vaccination Site With Irradiated MIP-3α–transfected Tumor Cells Enhances Efficacy of Dendritic Cell-based Cancer Vaccine

Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation

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