Administration of Microencapsulated Probiotic Bacillus sp. NP5 and Prebiotic Mannan Oligosaccharide for Prevention of Aeromonas hydrophila Infection on Pangasianodon hypophthalmus

Abstract

A feeding trial was conducted to investigate the effects of administration of microencapsulated probiotic Bacillus sp. NP5 and prebiotic mannan oligosaccharide (MOS) for the prevention of Aeromonas hydrophila infection on Pangasianodon hypophthalmus. Pangasius with initial body weight of 6.54±0.17 g were stocked into 40 L aquariums with a stocking density of 10 fish per aquarium. Control (C) diet (no addition of Bacillus sp. NP5 and MOS), probiotic Bacillus sp. NP5 1% (10 g kgG 1), prebiotic MOS 0.2% (2 g kgG 1) and synbiotic (probiotic Bacillus sp. NP5 1%+prebiotic MOS 0.2%). Then 10 fish of each aquarium were challenged by Aeromonas hydrophila. The results showed that fish with synbiotic supplementation resulted the highest Specific Growth Rate (SGR), Food Conversion Ratio (FCR) and those were significantly different (p<0.05) from the other treatment. The immune responses showed that fish fed with the control diet resulted the lowest hematocrit, hemoglobin and red blood cell count and those were significantly different from the probiotic and prebiotic group (p<0.05). White blood cell count, phagocytic activity and respiratory burst activity of the fish fed with probiotic 1% and MOS 0.2% were not significantly different from control. Moreover, fish fed with diet supplemented with probiotic, prebiotic and synbiotic had notably lower mortality after 10 days infected with A. hydrophila (p<0.05). Dietary Bacillus sp. NP5 and MOS had a significant interaction on enhancing immune responses and growth performances of Pangasianodon hypophthalmus (p<0.05).

Nile Tilapia Nursery in a Biofloc System: Evaluation of Different Stocking Densities

“…Changes in hematocrit (hemoconcentrated or hemodiluted) may be related to stress (Morgan et al, 1997). Decreased percentage of hematocrit, hemoglobin and erythrocytes may be caused by an infection resulting in red cell lysis (Tamamdusturi et al, 2016).…”

Supplementation of Nodulisporium sp. KT29 induced by Vibrio harveyi as an immunostimulant for controlling vibriosis in vannamei white shrimp under marine culture system

“…However, an excessive increase level of RB is regarded as an unfortunate condition for the host . The other studies also reported the beneficial factor of the immunostimulant to several different species, such as Cyprinus carpio (Lin et al, 2012), common carp (Djauhari et al, 2016), and Pangasianodon hypophthalmus (Tamamdusturi et al, 2016). Meanwhile, the decrease of immune response indicated recovery cell against V. harveyi.…”

The Application of Probiotic to Increase Growth, Body Composition, and Feed Efficiency on Catfish (Clarias sp.)

“…The beneficial effects of Bacillus genus as probiotic on aquaculture have been observed, such asBacillus spp. [26,30,31], Bacillus subtilis [22,32,33], Bacillus NP5 [10,12,13], Bacillus sp. [34], Bacillus licheniformis [22], Bacillus cereus [35], Bacillus mycoides [36], Bacillus coagulans [37].…”

“…The administration of Bacillus NP5 as probiotic has been reported could enhance growth performance of tilapia [9,10] and Dumbo catfish [11]. The supplementation of Bacillus NP5 also reported improve response immune of white shrimp [12] and catfish [13]. [14] noted that Bacillus NP5 is probiotic bacteria from digestive of tilapia.…”