Administration of Insulin to Rats Induces Cycloheximide-Sensitive Changes in the Calcium-Ion-Transport System of Mitochondria Isolated Subsequently from Liver

Barritt, Greg J.; Dorman, D M; Bygrave, Fyfe L.

doi:10.1042/bst0030711

Cited by 3 publications

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“…In conjunction with the plasma membrane and endoplasmic reticulum, mitochondria appear to play an important role in the regulation of intracellular Ca2+ concentrations through their ability to actively transport and accumulate this cation (for review see Bygrave, 1977). Our previous studies have shown that the administration of insulin to starved rats is associated with a stimulation of the initial rates of Ca2+ and phosphate transport, and an increase in the time for which Ca2+ is retained in mitochondria subsequently isolated from the liver (Dorman et al, 1975;Barritt et al, 1975Barritt et al, , 1978. Increases in the concentration of Ca2+ in isolated mitochondria were shown to have marked effects on the activity of the gluconeogenic enzyme, pyruvate carboxylase, and on the transport of pyruvate and ATP across the mitochondrial membrane (Foldes & Barritt, 1977).…”

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Effects of glucagon and N6O2′-dibutyryladenosine 3′:5′-cyclic monophosphate on calcium transport in isolated rat liver mitochondria

Hughes¹,

Barritt²

1978

Biochemical Journal

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1. The administration of glucagon to fed rats by intraperitoneal injection, or the perfusion of livers from fed rats with glucagon by the method of Mortimore [Mortimore (1963) Am.J. Physiol. 204, 699--704] was associated with increases of 15- and 5-fold respectively, in the time for which a given load of exogenous Ca2+ is retained by mitochondria subsequently isolated from the liver. This effect of glucagon was (a) also induced by N6O2'-dibutyryl cyclic AMP, (b) completely blocked by cycloheximide, (c) relatively slow in onset (15--60 min) and (d) associated with a stimulation of about 20% in the rates of ADP-stimulated oxygen utilization and Ca2+ transport measured in the presence of succinate. 2. Perfusion of livers with glucagon resulted in the isolation of mitochandria which showed a 50% increase, no significant change and a 40% increase in the concentrations of endogenous Ca, Mg and Pi respectively, when compared with mitochondria isolated from control perfused livers. 3. The administration of insulin or adrenaline to fed rats induced increases of 10- and 8-fold respectively, in the time for which Ca2+ is retained by isolated liver mitochondria. Perfusion of livers with insulin had no effect on mitochondrial Ca2+ retention time. 4. The perfusion of livers from starved rats with glucagon, or the administration of either glucagon or insulin to starved rats, increased by about 2.5- and 15-fold respectively, the time for which isolated mitochondria retain Ca2+. 5. Mechanisms which may be responsible for the observed alterations in Ca2+-retention time are discussed.

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mentioning

confidence: 98%

Effects of glucagon and N6O2′-dibutyryladenosine 3′:5′-cyclic monophosphate on calcium transport in isolated rat liver mitochondria

Hughes¹,

Barritt²

1978

Biochemical Journal

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Mitochondrial Calcium Transport

Bygrave¹

1977

Current Topics in Bioenergetics

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Effects of hormones and N6O2′-dibutyryl-adenosine 3′ :5′-cyclic monophosphate, administered in vivo, on phosphate transport and metabolism in isolated rat liver mitochondria

Barritt¹,

Thorne²,

Hughes³

1978

Biochemical Journal

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1. The administration of glucagon or N602'-dibutyryl cyclic AMP to fed rats by intraperitoneal injection was associated with a 2-fold increase in the amounts of endogenous Pi and ATP, and an increase in the rate and extent of transport of exogenous Pi (measured in either the presence or the absence of Ca2+) in mitochondria subsequently isolated from the liver. No change was observed in either the maximum rate of transport of exogenous Pi or in the rate of 32P1 exchange. 2. The changes induced by glucagon and dibutyryl cyclic AMP were markedly decreased by the co-administration of cycloheximide. 3. The administration of insulin to rats resulted in an increase of about 1.3-fold in the concentration of endogenous mitochondrial Pi. 4. The amounts of endogenous Pi in mitochondria isolated from the livers of starved rats were 3 times those in mitochondria isolated from fed animals. 5. It is concluded that the liver mitochondrial phosphatetransport system may be an important site of hormone action. 6. In the course of these experiments, it was shown that Ca2+ markedly stimulates mitochondrial phosphate transport.We have shown that the administration of insulin to starved rats by intraperitoneal injection is associated with an altered ability of mitochondria subsequently isolated from the liver to transport Ca2+ in vitro Barritt et al., 1975). The transport of Ca2+ by mitochondria is closely linked to the transport of phosphate ions. These anions enhance the rate of Ca2+ transport, increase the maximum amount of Ca2+ transported and alter the time for which Ca2+ ions are retained by isolated mitochondria (Lehninger, 1974;Reed & Bygrave, 1975;Bygrave, 1977

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Administration of Insulin to Rats Induces Cycloheximide-Sensitive Changes in the Calcium-Ion-Transport System of Mitochondria Isolated Subsequently from Liver

Cited by 3 publications

References 0 publications

Effects of glucagon and N6O2′-dibutyryladenosine 3′:5′-cyclic monophosphate on calcium transport in isolated rat liver mitochondria

Effects of glucagon and N6O2′-dibutyryladenosine 3′:5′-cyclic monophosphate on calcium transport in isolated rat liver mitochondria

Mitochondrial Calcium Transport

Effects of hormones and N6O2′-dibutyryl-adenosine 3′ :5′-cyclic monophosphate, administered in vivo, on phosphate transport and metabolism in isolated rat liver mitochondria

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