Administration of DHA-PS to Aged Mice Was Suitable for Increasing Hippocampal PS and DHA Ratio

Ohkubo, Tadayasu; Tanaka, Yukihisa

doi:10.5650/jos.59.247

Cited by 12 publications

(6 citation statements)

References 41 publications

(46 reference statements)

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“…PS derived from soy lecithin is currently given orally to adults at a dosage of 200–300 mg per day and to children at 520 mg per day. The substance has been tested in humans and animals at a higher dose than recommended for use in humans with no toxicity observed [42], [43], [44], [45]. The PS/DHA conjugate (Sharp·PS GOLD) increased the level of IKAP mRNA at much lower concentrations than the effective concentrations of PS in the FD cells used in our study.…”

Section: Discussionmentioning

confidence: 86%

Phosphatidylserine Increases IKBKAP Levels in Familial Dysautonomia Cells

et al. 2010

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Familial Dysautonomia (FD) is an autosomal recessive congenital neuropathy that results from abnormal development and progressive degeneration of the sensory and autonomic nervous system. The mutation observed in almost all FD patients is a point mutation at position 6 of intron 20 of the IKBKAP gene; this gene encodes the IκB kinase complex-associated protein (IKAP). The mutation results in a tissue-specific splicing defect: Exon 20 is skipped, leading to reduced IKAP protein expression. Here we show that phosphatidylserine (PS), an FDA-approved food supplement, increased IKAP mRNA levels in cells derived from FD patients. Long-term treatment with PS led to a significant increase in IKAP protein levels in these cells. A conjugate of PS and an omega-3 fatty acid also increased IKAP mRNA levels. Furthermore, PS treatment released FD cells from cell cycle arrest and up-regulated a significant number of genes involved in cell cycle regulation. Our results suggest that PS has potential for use as a therapeutic agent for FD. Understanding its mechanism of action may reveal the mechanism underlying the FD disease.

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Section: Discussionmentioning

confidence: 86%

Phosphatidylserine Increases IKBKAP Levels in Familial Dysautonomia Cells

et al. 2010

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“…Nevertheless, an increment in NAA concentration also induces many alterations such as oxidative stress, Canavan disease or genotoxicity and protein interaction due to an increase in nitric oxide produced by the elevated concentration of NAA (Surendran and Bhatnagar 2011 ). Compared with other organs, the brain has a high content of lipids, some two-thirds of which are phospholipids (Ohkubo and Tanaka 2010 ). Hippocampal pyramidal neurons represent by far the most abundant type of neuron in the hippocampus, and their dendritic arbor is covered by dendritic spines.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus

et al. 2017

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Understanding the human brain is the ultimate goal in neuroscience, but this is extremely challenging in part due to the fact that brain tissue obtained from autopsy is practically the only source of normal brain tissue and also since changes at different levels of biological organization (genetic, molecular, biochemical, anatomical) occur after death due to multiple mechanisms. Here we used metabolomic and anatomical techniques to study the possible relationship between post-mortem time (PT)-induced changes that may occur at both the metabolomics and anatomical levels in the same brains. Our experiments have mainly focused on the hippocampus of the mouse. We found significant metabolomic changes at 2 h PT, whereas the integrity of neurons and glia, at the anatomical/ neurochemical level, was not significantly altered during the first 5 h PT for the majority of histological markers.Electronic supplementary materialThe online version of this article (doi:10.1007/s00429-017-1375-5) contains supplementary material, which is available to authorized users.

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“…Liposomes prepared from natural lipid mixtures extracted from marine animals appeared to be superior candidates for DHA/EPA supplementation. The greater bioavailability of omega-3 PUFAs measured after phospholipid liposome ingestion, compared with triglycerides ingestion, is supported by a number of animal and clinical studies [5–7]. It has been also reported that decreases in brain plasmalogen phospholipids, one of the earliest biochemical changes detected in Alzheimer disease (AD), are specific to brain regions with AD pathology [8], and that the plasmalogen ether phospholipid precursors can be supplied by dietary supplements.…”

Section: Introductionmentioning

confidence: 99%

Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry

Chen¹,

Belikova

Subbaiah

2012

Analytica Chimica Acta

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Although marine oysters contain abundant amounts of ether-linked aminophospholipids, the structural identification of the various molecular species has not been reported. We developed a normal-phase silica liquid chromatography/negative-ion electrospray ionization/quadrupole multiple-stage linear ion-trap mass spectrometric (NPLC-NI-ESI/Q-TRAP-MS3) method for the structural elucidation of ether molecular species of serine and ethanolamine phospholipids from marine oysters. The major advantages of the approach are (i) to avoid incorrect selection of isobaric precursor ions derived from different phospholipid classes in a lipid mixture, and to generate informative and clear MSn product ion mass spectra of the species for the identification of the sn-1 plasmanyl or plasmenyl linkages, and (ii) to increase precursor ion intensities by “concentrating” lipid molecules of each phospholipid class for further structural determination of minor molecular species. Employing a combination of NPLC-NI-ESI/MS3 and NPLC-NI-ESI/MS2, we elucidated, for the first time, the chemical structures of docosahexaenoyl and eicosapentaenoyl plasmenyl phosphatidylserine (PS) species and differentiated up to six isobaric species of diacyl/alkylacyl/alkenylacyl phosphatidylethanolamine (PE) in the US pacific oysters. The presence of a high content of both omega-3 plasmenyl PS/plasmenyl PE species and multiple isobaric molecular species isomers is the noteworthy characteristic of the marine oyster. The simple and robust NPLC-NI-ESI/MSn-based methodology should be particularly valuable in the detailed characterization of marine lipid dietary supplements with respect to omega-3 aminophospholipids.

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Administration of DHA-PS to Aged Mice Was Suitable for Increasing Hippocampal PS and DHA Ratio

Cited by 12 publications

References 41 publications

Phosphatidylserine Increases IKBKAP Levels in Familial Dysautonomia Cells

Phosphatidylserine Increases IKBKAP Levels in Familial Dysautonomia Cells

Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus

Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry

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