Administration of butorphanol with ketamine/xylazine sedation reduces the negative responses to electroejaculation in rams

Ungerfeld, Rodolfo; Fernandes, Daniel Andrews de Moura; Balaro, M. F. A.; Taira, Akihiro; Santo, Caroline Gomes do Espírito; Santos, Juliana Dantas Rodrigues; Costa, Marta Maria Campos Pereira da; Carvalho, Ana Beatriz da Silva; Rodrigues, A. L. R.; Brandão, Felipe Zandonadi

doi:10.1016/j.theriogenology.2022.08.008

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…Conversely, Ungerfeld et al. (2022) reported an increase in the stimulus quantity after the administration of butorphanol prior to EE in rams. Similar results were observed in our study, where the desensitization of the innervation involved in the ejaculatory process led to an increase in the number of electrical stimuli in certain treatments (T2 and T4; Table 3), resulting in a higher number of involuntary muscle contractions.…”

Section: Discussionmentioning

confidence: 96%

“…Ungerfeld et al (2018) observed a reduction in the average number of electrical stimuli required to induce ejaculation after the administration of cloprostenol and oxytocin prior to EE in goat breeders. Conversely,Ungerfeld et al (2022) reported an increase in the stimulus quantity after the administration of butorphanol prior to EE in rams. Similar results were observed in our study, where the desensitization of the innervation involved in the ejaculatory process led to an increase in the number of electrical stimuli in certain treatments (T2 and T4;…”

mentioning

confidence: 95%

“…The use of electroejaculation (EE) technique is often required in the small ruminant production system to assess semen quality during non‐breeding season, or when males are not trained for semen collection using artificial vagina (Romano et al., 2021; Ungerfeld et al., 2022). The technique consists of electrical pulse stimulation of branches of the hypogastric plexus (Semans & Langworthy, 1983) that surrounds the anterior pelvis and accessory glands, inducing the emission of semen and stimulating strong muscular contractions in the pelvic muscles (Damián & Ungerfeld, 2011; Vidal et al., 2007).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of pharmacological alternatives to reduce the pain and discomfort produced by electroejaculation in rams

da Silva,

de Almeida Gélio,

Oba

et al. 2024

Reprod Domestic Animals

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Electroejaculation (EE) represents the main technique for semen collection from domestic and wild animals independently of libido. However, the technique is associated with intense involuntary muscle contractions, vocalization, ataxia and lying down, caused by the electric stimulation of the nerves in the caudal epigastric region. These clinical manifestations represent important indicators of discomfort. In this context, the objective of this study was to evaluate two protocols of local anaesthetic blockade and two anatomical access for pharmacological desensitization of the caudal epigastric innervation as alternatives to promote comfort and reduce stress associated with EE in rams. For the study, four clinically healthy Dorper rams were selected. All animals were subjected to a design consisting of five semen collection treatments (n = 3 collections per treatment): T1—control, conventional EE without local anaesthetic blockade; T2, EE with ventral blockade (VB) of epigastric innervation using lidocaine hydrochloride 2%; T3, EE with VB of epigastric innervation using a combination of lidocaine hydrochloride 2% and fentanyl citrate; T4, EE with blockade of epigastric innervation through the perineal access using lidocaine hydrochloride 2%; T5, EE with blockade of epigastric innervation through the perineal access using a combination of lidocaine hydrochloride and fentanyl citrate. Seminal samples resulting from EE were subjectively evaluated for sperm motility and concentration, vigour and volume. Additionally, blood serum samples were collected for quantification of cortisol and creatine kinase (CK) enzyme. Assessments of stress and discomfort were conducted by measuring blood pressure, heart rate (HR) and respiratory rate (RR), as well as observing involuntary muscle contractions, ataxia and animal vocalization. No variations in blood pressure, sperm motility, vigour, CK, and cortisol were observed among the treatments. Individual variations were observed for the occurrence of vocalization (p = .0066), but there were no differences between the groups. Anaesthetic blockades conducted using the combination of lidocaine and fentanyl resulted in a lower incidence of ataxia during EE (p < .0001). It is concluded that the combination of fentanyl citrate and lidocaine hydrochloride results in less discomfort for animals undergoing EE, regardless of the anatomical access used for local anaesthetic blockades.

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Section: Discussionmentioning

confidence: 96%

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confidence: 95%