Administration of bacterial lipopolysaccharide elicits circulating tumor necrosis factor-alpha in neonatal calves

Adams, Jacquelyn H.; Semrad, Susan D.; Czuprynski, Charles J.

doi:10.1128/jcm.28.5.998-1001.1990

Cited by 37 publications

(15 citation statements)

References 8 publications

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“…This might be explained by the kinetics of TNF-a, which is known to be produced and degraded rather quickly. Thus, the level of the cytokine easily can change within hours (Adams et al, 1990;Horadagoda et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Increased pulmonary secretion of tumor necrosis factor-α in calves experimentally infected with bovine respiratory syncytial virus

Røntved¹,

Tjørnehøj²,

Viuff³

et al. 2000

Veterinary Immunology and Immunopathology

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Bovine respiratory syncytial virus (BRSV) is an important cause of respiratory disease among calves in the Danish cattle industry. An experimental BRSV infection model was used to study the pathogenesis of the disease in calves. Broncho alveolar lung lavage (BAL) was performed on 28 Jersey calves, of which 23 were experimentally infected with BRSV and five were given a mock inoculum. The presence of the cytokine tumor necrosis factor alpha (TNF-alpha) in the BAL fluids was detected and quantified by a capture ELISA. TNF-alpha was detected in 21 of the infected animals. The amount of TNF-alpha in the BAL fluid of calves killed post inoculation day (PID) 2 and 4 was at the same very low level as in the uninfected control animals. Large amounts of TNF-alpha were detected on PID 6, maximum levels of TNF-alpha were reached on PID 7, and smaller amounts of TNF-alpha were seen on PID 8. The high levels of TNF-alpha appeared on the days where severe lung lesions and clinical signs were obvious and the amounts of BRSV-antigen were at their greatest. Although Pasteurellaceae were isolated from some of the BRSV-infected calves, calves treated with antibiotics before and through the whole period of the infection, as well as BRSV-infected calves free of bacteria reached the same level of TNF-alpha as animals from which bacteria were isolated from the lungs. It is concluded that significant quantities of TNF-alpha are produced in the lungs of the calves on PID 6-7 of BRSV infection. The involvement of TNF-alpha in the pathogenesis of, as well as the anti-viral immune response against, BRSV infection is discussed.

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Section: Discussionmentioning

confidence: 99%

Increased pulmonary secretion of tumor necrosis factor-α in calves experimentally infected with bovine respiratory syncytial virus

Røntved¹,

Tjørnehøj²,

Viuff³

et al. 2000

Veterinary Immunology and Immunopathology

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“…This results in the fact that Gram-negative sepsis is associated with high mortality rates, despite comprehensive treatment in intensive care patients [1]. Studies are underway with regards to understanding the complex pathophysiological mechanism of endotoxemia, describing criteria for early diagnosis and developing new treatment approaches in order to decrease mortality [2,3].…”

Section: Introductionmentioning

confidence: 99%

Changes in serum proteins after endotoxin administration in healthy and choline-treated calves

et al. 2016

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BackgroundThis study aimed to investigate the possible serum protein changes after endotoxin administration in healthy and choline-treated calves using proteomics. These results are expected to contribute to the understanding of the pathophysiological mechanisms of endotoxemia and the beneficial effect of choline administration in this clinical situation.MethodsHealthy-calves (n = 20) were divided into 4 groups: Control, Choline treated (C), Lipopolysaccharide administered (LPS), and LPS + C. Control calves received 0.9 % NaCl injection. Calves in C and LPS + C groups received choline chloride (1 mg/kg/iv). Endotoxin (LPS) was injected (2 μg/kg/iv) to the calves in LPS and LPS + C groups. Serum samples were collected before and after the treatments. Differentially expressed proteins (> 1.5 fold-change relative to controls) were identified by LC-MS/MS.ResultsAfter LPS administration, 14 proteins increased, and 13 proteins decreased within 48 h as compared to controls. In the LPS group, there were significant increases in serum levels of ragulator complex protein (189-fold) and galectin-3-binding protein (10-fold), but transcription factor MafF and corticosteroid binding globulin were down regulated (≥ 5 fold). As compared with the LPS group, in LPS + C group, fibrinogen gamma-B-chain and antithrombin were up-regulated, while hemopexin and histone H4 were down-regulated. Choline treatment attenuated actin alpha cardiac muscle-1 overexpression after LPS.ConclusionsLPS administration produces changes in serum proteins associated with lipid metabolism, immune and inflammatory response, protein binding/transport, cell adhesion, venous thrombosis, cardiac contractility and blood coagulation. The administration of choline is associated with changes in proteins which can be related with its beneficial effect in this clinical situation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0837-y) contains supplementary material, which is available to authorized users.

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“…13 Serum TNF concentration significantly increased in all endotoxin-treated calves. [12][13][14][15][16][17][18][19][20][21] At low concentration, TNF exerts its primary effects locally as a paracrine and autocrine regulator of leukocytes and endothelial cells. These actions are critical for the containment of infection.…”

Section: Discussionmentioning

confidence: 99%

“…16 Although many models are used to study the problem, none completely simulates the natural disease in neonatal calves. [12][13][14][15][17][18][19][20][21] Because gram-negative bacterial septicemia is accompanied by high serum TNF concentration in experimental animals models, 22 an association between serum TNF concentration and severity of disease in neonatal calves was considered likely. The present study was undertaken to determine whether high circulating concentrations of TNF-␣ are found during naturally occurring disease suggestive of septicemia in neonatal calves.…”

mentioning

confidence: 99%

Serum Concentrations of Tumor Necrosis Factor‐a in Neonatal Calves with Presumed Septicemia

Basoglu

Şen

Sevinc

et al. 2004

Veterinary Internal Medicne

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This study was performed to determine the concentrations of tumor necrosis factor (TNF) in the serum of neonatal calves with presumed sepsis and determine the correlation between serum concentrations of TNF and the severity and outcome of disease. Thirty-five sick calves < 30 days old that suffered from enteritis, respiratory disease, or both were considered suitable for inclusion in this study by satisfying clinical and laboratory criteria suggestive of septicemia. At admission, blood samples were collected from all calves to determine the prevalence of high concentrations of TNF. The clinical course and outcome of disease then were recorded. Of the 35 calves with presumed sepsis, 10 had high serum TNF concentrations. Scleral injection, weak or absent suckling reflex, sternal or lateral recumbency, unresponsive or comatose state, and death rate of calves with high serum TNF concentration were greater than those values for calves without high serum TNF concentration. Calves with high serum TNF concentration had significantly lower mean IgG (P < .001), globulin (P < .0001), and calcium (P < .0001) concentrations; greater serum creatinine concentrations (P < .0001); and > or = 2+ toxic changes in neutrophils than did calves without high serum TNF concentrations. Mean values for packed cell volume, band neutrophil count, and venous Pco2 were significantly (P < .007) higher in the group of calves with high serum TNF concentration. Results of this study indicate that serum TNF concentration is correlated with clinical criteria of sepsis in neonatal calves. A close association was apparent between disease severity and serum TNF concentrations in this group of calves with presumed septicemia.

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Administration of bacterial lipopolysaccharide elicits circulating tumor necrosis factor-alpha in neonatal calves

Cited by 37 publications

References 8 publications

Increased pulmonary secretion of tumor necrosis factor-α in calves experimentally infected with bovine respiratory syncytial virus

Increased pulmonary secretion of tumor necrosis factor-α in calves experimentally infected with bovine respiratory syncytial virus

Changes in serum proteins after endotoxin administration in healthy and choline-treated calves

Serum Concentrations of Tumor Necrosis Factor‐a in Neonatal Calves with Presumed Septicemia

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