Administration of antisense DNA for ghrelin causes an antidepressant and anxiolytic response in rats

Kanehisa, Masayuki; Akiyoshi, Jotaro; Kitaichi, Tomoko; Matsushita, Hirotaka; Tanaka, Etsuhiro; Kodama, Kensuke; Hanada, Hiroaki; Isogawa, Koichi

doi:10.1016/j.pnpbp.2006.05.005

Cited by 78 publications

(62 citation statements)

References 23 publications

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“…In addition, the anxiogenic effect of GHRL was reported in a rat model 11 and an antidepressantlike effect of anti-sense DNA for ghrelin in rats may suggest a depressogenic effect. 31 However, in our previous study, the Gln90Leu genotype was not linked to acylated GHRL levels in H. pylori-negative FD patients. 22 In contrast, Ando et al 32 have…”

Section: Discussionmentioning

confidence: 71%

“…4,35,36 In addition, Kusano et al 6 reported that early phase of gastric emptying is also contributed to FD patients as well as delayed gastric emptying. 31 Lee et al 30 reported that gastric flow into the duodenum including gastric acid inhibits gastric accommodation to a meal and contributes to postprandial symptom. Considering these reports, it warrants further exploration to clarify the precise mechanisms underlying impaired gastric emptying in early postprandial phase to truly understand the pathophysiology of FD.…”

Section: Discussionmentioning

confidence: 99%

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Leu72Met408 Polymorphism of the Ghrelin Gene Is Associated With Early Phase of Gastric Emptying in the Patients With Functional Dyspepsia in Japan

Yanagawa

Futagami

Shimpuku

et al. 2015

J Neurogastroenterol Motil

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Background/Aims There are no available data about the relationship between ghrelin gene genotypes and early phase of gastric emptying in functional dyspepsia (FD) as defined by Rome III classification. Methods We enrolled 74 patients presenting with typical symptoms of FD and 64 healthy volunteers. Gastric motility was evaluated using the 13 C-acetate breath test. We used Rome III criteria to evaluate upper abdominal symptoms and self-rating questionnaires for depression (SRQ-D) scores to determine status of depression. The Arg51Gln (346G>A), preproghrelin (3056T>C), Leu72Met (408C>A), Gln90Leu (3412T>A) and G-protein β3 (825C>T) polymorphisms were analyzed in the DNA from blood samples of enrolled subjects. Genotyping was performed by polymerase chain reaction. Results There was a significant relationship between the Gln90Leu3412 genotype and SRQ-D score in FD patients ( P = 0.009). Area under the curve at 15 minutes (AUC 15 ) value was significantly associated with the Leu72Met408 genotype ( P = 0.015) but not with entire gastric emptying. Conclusions The Leu72Met (408C>A) single nucleotide polymorphism was significantly associated with early phase of gastric emptying in FD patients. Further studies will be necessary to clarify the association between ghrelin gene single nucleotide polymorphisms and early phase of gastric emptying in FD patients.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Leu72Met408 Polymorphism of the Ghrelin Gene Is Associated With Early Phase of Gastric Emptying in the Patients With Functional Dyspepsia in Japan

Yanagawa

Futagami

Shimpuku

et al. 2015

J Neurogastroenterol Motil

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“…Masayuki Kanehisa reported that in forced swimming tests, rats that received antisense DNA for ghrelin, immobilization time decreased. Also, Ghrelin antisense oligonucleotides produced an anxiolytic-like effects in the elevated plus maze test, black and white test, or conditioned fear tests (Kanehisa et al 2006).…”

Section: Discussionmentioning

confidence: 98%

Effects of Toxoplasma gondii infection on anxiety, depression and ghrelin level in male rats

et al. 2014

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The aim of the present study was to determine the effects of Toxoplasma gondii (T. gondii) infection on anxiety, depression and ghrelin level in male rats. Twenty four male rats were assessed in two equal groups. T. gondii tachyzoite (ip) were injected in infected group and control group received (2 ml) physiological serum (ip). Elevated plus Maze and swimming tests were used to assess anxiety and depression in rats respectively. The ghrelin and T. gondii IgG serum levels were measured by enzyme immunoassay kits. The Student's t test and Pearson correlation coefficient were used. The ghrelin serum level was significantly lower in the infected rats than control (P = 0.03). There were no significant differences in the depression and anxiety behavior between two groups. However, here were no significant correlations between ghrelin level and anxiety or depression in rats. It seems that latent T. gondii infection decreases the ghrelin serum level but does not change anxiety and depression like behaviors.

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“…In rodents, acute peripheral, as well as central ghrelin injection (both ICV and site specific: amygdala, dorsal raphe nucleus, hippocampus) induce anxiety-like behaviour (Asakawa et al, 2001, Carlini et al, 2002, Carlini et al, 2004. Furthermore, suppression of central ghrelin action by administration of antisense DNA for ghrelin caused a decrease in anxiety-and depression-like behaviour in rats (Kanehisa et al, 2006). In contrast to these findings, Lutter and colleagues reported a decrease in anxiety-and depression-like behaviour in mice after peripheral ghrelin injection as well as after starvation (Lutter et al, 2008).…”

Section: 2mentioning

confidence: 99%

Central administration of ghrelin alters emotional responses in rats: behavioural, electrophysiological and molecular evidence

et al. 2011

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Administration of antisense DNA for ghrelin causes an antidepressant and anxiolytic response in rats

Cited by 78 publications

References 23 publications

Leu72Met408 Polymorphism of the Ghrelin Gene Is Associated With Early Phase of Gastric Emptying in the Patients With Functional Dyspepsia in Japan

Leu72Met408 Polymorphism of the Ghrelin Gene Is Associated With Early Phase of Gastric Emptying in the Patients With Functional Dyspepsia in Japan

Effects of Toxoplasma gondii infection on anxiety, depression and ghrelin level in male rats

Central administration of ghrelin alters emotional responses in rats: behavioural, electrophysiological and molecular evidence

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